{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,24]],"date-time":"2026-04-24T11:42:02Z","timestamp":1777030922972,"version":"3.51.4"},"reference-count":26,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2019,10,25]],"date-time":"2019-10-25T00:00:00Z","timestamp":1571961600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81671413"],"award-info":[{"award-number":["81671413"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81671414"],"award-info":[{"award-number":["81671414"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["obgyn.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Acta Obstet Gynecol Scand"],"published-print":{"date-parts":[[2020,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Introduction<\/jats:title><jats:p>Polycystic ovary syndrome is a complex endocrine condition with chronic inflammation. Prostaglandin E2 (<jats:styled-content style=\"fixed-case\">PGE<\/jats:styled-content>2) is a proinflammatory factor with an increased expression in the serum of women with polycystic ovary syndrome. <jats:italic>Zinc finger gene 217<\/jats:italic> (<jats:italic><jats:styled-content style=\"fixed-case\">ZNF<\/jats:styled-content>217<\/jats:italic>) is known as a candidate gene for polycystic ovary syndrome. We aimed to investigate the relation between <jats:italic><jats:styled-content style=\"fixed-case\">ZNF<\/jats:styled-content>217<\/jats:italic> and <jats:styled-content style=\"fixed-case\">PGE<\/jats:styled-content>2 in polycystic ovary syndrome.<\/jats:p><\/jats:sec><jats:sec><jats:title>Material and methods<\/jats:title><jats:p>We used a rat model of dehydroepiandrosterone\u2010induced polycystic ovary syndrome and human granulosa cells both of women with polycystic ovary syndrome and of women without the syndrome to measure <jats:italic><jats:styled-content style=\"fixed-case\">ZNF<\/jats:styled-content>217<\/jats:italic> and other target gene expressions. In addition, we performed in vitro experiments with <jats:styled-content style=\"fixed-case\">KGN<\/jats:styled-content> human granulosa\u2010like tumor cells to verify the molecular mechanisms.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p><jats:italic><jats:styled-content style=\"fixed-case\">ZNF<\/jats:styled-content>217<\/jats:italic> was decreased in the granulosa cells both of dehydroepiandrosterone\u2010treated rats and of women with polycystic ovary syndrome. Cyclooxygenase 2, a key enzyme of <jats:styled-content style=\"fixed-case\">PGE<\/jats:styled-content>2 synthesis, was highly expressed in the granulosa cells of rats and women with the syndrome, and <jats:styled-content style=\"fixed-case\">PGE<\/jats:styled-content>2 concentration was increased in the follicular fluid. Furthermore, decreased <jats:italic><jats:styled-content style=\"fixed-case\">ZNF<\/jats:styled-content>217<\/jats:italic> expression was supposed to inhibit estradiol synthesis, which further promoted cyclooxygenase 2 and <jats:styled-content style=\"fixed-case\">PGE<\/jats:styled-content>2 synthesis. At the same time, <jats:styled-content style=\"fixed-case\">PGE<\/jats:styled-content>2 had an inhibitory effect on <jats:italic><jats:styled-content style=\"fixed-case\">ZNF<\/jats:styled-content>217<\/jats:italic> expression in a dose\u2010dependent manner in <jats:styled-content style=\"fixed-case\">KGN<\/jats:styled-content> cells.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Decreased <jats:italic><jats:styled-content style=\"fixed-case\">ZNF<\/jats:styled-content>217<\/jats:italic> expression in granulosa cells of women with polycystic ovary syndrome induced inflammation via <jats:styled-content style=\"fixed-case\">PGE<\/jats:styled-content>2, and <jats:styled-content style=\"fixed-case\">PGE<\/jats:styled-content>2 inhibited <jats:italic><jats:styled-content style=\"fixed-case\">ZNF<\/jats:styled-content>217<\/jats:italic> expression to establish a feedback loop. This mechanism might account for the pathogenesis of polycystic ovary syndrome.<\/jats:p><\/jats:sec>","DOI":"10.1111\/aogs.13719","type":"journal-article","created":{"date-parts":[[2019,8,27]],"date-time":"2019-08-27T16:29:46Z","timestamp":1566923386000},"page":"119-126","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["A candidate pathogenic gene, zinc finger gene <i>217<\/i> (<i><scp>ZNF<\/scp>217<\/i>), may contribute to polycystic ovary syndrome through prostaglandin E2"],"prefix":"10.1111","volume":"99","author":[{"given":"Junyu","family":"Zhai","sequence":"first","affiliation":[{"name":"Center for Reproductive Medicine Ren Ji Hospital School of Medicine Shanghai Jiao Tong University Shanghai China"},{"name":"Shanghai Key Laboratory for Assisted Reproduction and Reproductive Genetics Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4363-0902","authenticated-orcid":false,"given":"Shang","family":"Li","sequence":"additional","affiliation":[{"name":"Center for Reproductive Medicine Ren Ji Hospital School of Medicine Shanghai Jiao Tong University Shanghai China"},{"name":"Shanghai Key Laboratory for Assisted Reproduction and Reproductive Genetics Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaoyue","family":"Cheng","sequence":"additional","affiliation":[{"name":"Center for Reproductive Medicine Ren Ji Hospital School of Medicine Shanghai Jiao Tong University Shanghai China"},{"name":"Shanghai Key Laboratory for Assisted Reproduction and Reproductive Genetics Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zi\u2010Jiang","family":"Chen","sequence":"additional","affiliation":[{"name":"Center for Reproductive Medicine Ren Ji Hospital School of Medicine Shanghai Jiao Tong University Shanghai China"},{"name":"Shanghai Key Laboratory for Assisted Reproduction and Reproductive Genetics Shanghai China"},{"name":"National Research Center for Assisted Reproductive Technology and Reproductive Genetics The Key Laboratory for Reproductive Endocrinology of Ministry of Education Shandong Provincial Key Laboratory of Reproductive Medicine Center for Reproductive Medicine Shandong Provincial Hospital Shandong University Jinan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Weiping","family":"Li","sequence":"additional","affiliation":[{"name":"Center for Reproductive Medicine Ren Ji Hospital School of Medicine Shanghai Jiao Tong University Shanghai China"},{"name":"Shanghai Key Laboratory for Assisted Reproduction and Reproductive Genetics Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yanzhi","family":"Du","sequence":"additional","affiliation":[{"name":"Center for Reproductive Medicine Ren Ji Hospital School of Medicine Shanghai Jiao Tong University Shanghai China"},{"name":"Shanghai Key Laboratory for Assisted Reproduction and Reproductive Genetics Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2019,10,25]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/deh098"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/imj.12495"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1530\/REP-18-0583"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.steroids.2011.12.003"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1530\/REP-14-0435"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.3109\/09513590.2012.760195"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.plipres.2010.07.005"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1124\/pr.59.3.1"},{"key":"e_1_2_9_10_1","first-page":"3611","article-title":"Expression of prostaglandin metabolising enzymes COX\u20102 and 15\u2010PGDH and VDR in human granulosa cells","volume":"29","author":"Thill M","year":"2009","journal-title":"Anticancer Res"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1093\/molehr\/5.11.1011"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0090-6980(96)00096-2"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1093\/molehr\/gal057"},{"key":"e_1_2_9_14_1","first-page":"333","article-title":"Amplification of zinc finger gene 217 (ZNF217) and cancer: when good fingers go bad","volume":"1775","author":"Quinlan KG","year":"2007","journal-title":"Biochim Biophys Acta"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tem.2014.12.004"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-017-03555-6"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11010-010-0469-7"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1523236113"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1210\/en.2014-1196"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep26691"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12020-016-0961-2"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2164-15-520"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tem.2005.08.005"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.13-228858"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-0035-1556568"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1210\/jc.2015-2031"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.3892\/mmr.2015.3901"}],"container-title":["Acta Obstetricia et Gynecologica Scandinavica"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/aogs.13719","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/aogs.13719","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/obgyn.onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/aogs.13719","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,7]],"date-time":"2023-09-07T14:06:33Z","timestamp":1694095593000},"score":1,"resource":{"primary":{"URL":"https:\/\/obgyn.onlinelibrary.wiley.com\/doi\/10.1111\/aogs.13719"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,10,25]]},"references-count":26,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2020,1]]}},"alternative-id":["10.1111\/aogs.13719"],"URL":"https:\/\/doi.org\/10.1111\/aogs.13719","archive":["Portico"],"relation":{},"ISSN":["0001-6349","1600-0412"],"issn-type":[{"value":"0001-6349","type":"print"},{"value":"1600-0412","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,10,25]]},"assertion":[{"value":"2019-03-02","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-08-20","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-10-25","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}