{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T04:45:16Z","timestamp":1791002716011,"version":"4.1.0"},"reference-count":21,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2020,5,17]],"date-time":"2020-05-17T00:00:00Z","timestamp":1589673600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100009708","name":"Novo Nordisk Fonden","doi-asserted-by":"publisher","award":["NNF16OC0018772"],"award-info":[{"award-number":["NNF16OC0018772"]}],"id":[{"id":"10.13039\/501100009708","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["obgyn.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Acta Obstet Gynecol Scand"],"published-print":{"date-parts":[[2020,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Introduction<\/jats:title><jats:p>Circulating fetal extravillous trophoblasts may offer a superior alternative to cell\u2010free fetal DNA for noninvasive prenatal testing. Cells of fetal origin are a pure source of fetal genome; hence, unlike the cell\u2010free noninvasive prenatal test, the fetal cell\u2010based noninvasive prenatal test is not expected to be affected by maternal DNA. However, circulating fetal cells from previous pregnancies may lead to confounding results.<\/jats:p><\/jats:sec><jats:sec><jats:title>Material and methods<\/jats:title><jats:p>To study whether fetal trophoblast cells persist in maternal circulation postpartum, blood samples were collected from 11 women who had given birth to a boy, with blood sampling at 1\u20103\u00a0days (W0), 4\u20105\u00a0weeks (W4\u20105), around 8\u00a0weeks (W8) and around 12\u00a0weeks (W12) postpartum. The existence of fetal extravillous trophoblasts was verified either by X and Y chromosome fluorescence in situ hybridization analysis or by short tandem repeat analysis. To exclude technological bias in isolating fetal cells, blood samples were also collected from 10 pregnant women between a gestational age of 10 and 14\u00a0weeks, the optimal time frame for cell\u2010based noninvasive prenatal test sampling. All the samples were processed according to protocols established by ARCEDI Biotech for fetal extravillous trophoblast enrichment and isolation.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Fetal extravillous trophoblasts were found in all the 10 samples from pregnant women between a gestational age of 10 and 14\u00a0weeks. However, only 4 of 11 blood samples taken from women at 1\u20103\u00a0days postpartum rendered fetal extravillous trophoblasts, and only 2 of 11 samples rendered fetal extravillous trophoblasts at 4\u00a0weeks postpartum.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>In this preliminary dataset on few pregnancies, none of the samples rendered any fetal cells at or after 8\u00a0weeks postpartum, showing that cell\u2010based noninvasive prenatal testing based on fetal extravillous trophoblasts is unlikely to be influenced by circulating cells from previous pregnancies.<\/jats:p><\/jats:sec>","DOI":"10.1111\/aogs.13880","type":"journal-article","created":{"date-parts":[[2020,4,23]],"date-time":"2020-04-23T07:53:20Z","timestamp":1587628400000},"page":"751-756","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Do fetal extravillous trophoblasts circulate in maternal blood postpartum?"],"prefix":"10.1111","volume":"99","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9086-1506","authenticated-orcid":false,"given":"Anne","family":"van de Looij","sequence":"first","affiliation":[{"name":"Department of Women's Disease and Birth Aarhus University Hospital Aarhus Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2657-0661","authenticated-orcid":false,"given":"Ripudaman","family":"Singh","sequence":"additional","affiliation":[{"name":"ARCEDI Biotech ApS Vejle Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0141-9172","authenticated-orcid":false,"given":"Lotte","family":"Hatt","sequence":"additional","affiliation":[{"name":"ARCEDI Biotech ApS Vejle 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