{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,13]],"date-time":"2026-02-13T11:08:40Z","timestamp":1770980920450,"version":"3.50.1"},"reference-count":32,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2021,1,12]],"date-time":"2021-01-12T00:00:00Z","timestamp":1610409600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100006197","name":"Fonden til L\u00e6gevidenskabens Fremme","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100006197","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["obgyn.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Acta Obstet Gynecol Scand"],"published-print":{"date-parts":[[2021,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Introduction<\/jats:title><jats:p>In pregnancy, vitamin D deficiency is associated with increased risk of fetal growth restriction and preeclampsia. The underlying mechanisms are not known, but placental dysfunction is believed to play a role. In a Danish population, where health authorities recommend a 10\u00a0\u00b5g\/day vitamin D supplement during pregnancy, we explored current use of vitamin D supplements and vitamin D status. In term placentas, alterations in vitamin D metabolism and placental growth, evaluated by the key placental growth factor pregnancy\u2010associated plasma protein\u2010A (PAPP\u2010A), and their relation to vitamin D insufficiency were investigated.<\/jats:p><\/jats:sec><jats:sec><jats:title>Material and methods<\/jats:title><jats:p>We included 225 randomly selected pregnant women attending a nuchal translucency scan at gestational weeks 11\u201014. Information on use of vitamin D supplements and body mass index (BMI) at inclusion was obtained using self\u2010reported questionnaires. Plasma 25\u2010hydroxyvitamin D was measured at inclusion and correlated with pregnancy outcomes and placental biology, as judged by expression of PAPP\u2010A and enzymes involved in vitamin D metabolism (CYP24A1, CYP27B1) in term placentas.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Vitamin D supplements were used by 92% of the women, but 42% were vitamin D insufficient (plasma 25\u2010hydroxyvitamin D &lt;75\u00a0nmol\/L). Eleven women with singleton pregnancies developed fetal growth restriction or preeclampsia. In this small subset, first\u2010trimester mean plasma 25\u2010hydroxyvitamin D was lower in women who developed fetal growth restriction (43\u00a0\u00b1\u00a033nmol\/L; n\u00a0=\u00a03; <jats:italic>P<\/jats:italic>\u00a0=\u00a0.006) and there was a tendency towards lower plasma 25\u2010hydroxyvitamin D among women who developed preeclampsia (65\u00a0\u00b1\u00a019\u00a0nmol\/L; n\u00a0=\u00a08; <jats:italic>P<\/jats:italic>\u00a0=\u00a0.08) in third trimester compared with uncomplicated pregnancies (79\u00a0\u00b1\u00a022\u00a0nmol\/L; n\u00a0=\u00a0187). In term placentas, PAPP\u2010A expression was lower among participants with first\u2010trimester vitamin D insufficiency (<jats:italic>P<\/jats:italic>\u00a0=\u00a0.009; n\u00a0=\u00a030) but no correlation was found between plasma 25\u2010hydroxyvitamin D and mRNA expression of CYP24A1 (<jats:italic>P<\/jats:italic>\u00a0=\u00a0.67) and CYP27B1 (<jats:italic>P<\/jats:italic>\u00a0=\u00a0.34). BMI was negatively correlated with plasma 25\u2010hydroxyvitamin D (<jats:italic>P<\/jats:italic>\u00a0=\u00a0.03) and positively correlated with placental mRNA expression of CYP24A1 (<jats:italic>P<\/jats:italic>\u00a0=\u00a0.003; n\u00a0=\u00a030).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Despite high compliance with official guidelines regarding vitamin D supplements, vitamin D insufficiency was frequent and the findings indicate that vitamin D insufficiency may affect placental growth. High BMI was associated with vitamin D insufficiency and increased placental vitamin D turnover, but further investigations are needed.<\/jats:p><\/jats:sec>","DOI":"10.1111\/aogs.14019","type":"journal-article","created":{"date-parts":[[2020,10,8]],"date-time":"2020-10-08T18:33:03Z","timestamp":1602181983000},"page":"480-488","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":23,"title":["Vitamin D insufficiency among Danish pregnant women\u2014Prevalence and association with adverse obstetric outcomes and placental vitamin D metabolism"],"prefix":"10.1111","volume":"100","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8101-8573","authenticated-orcid":false,"given":"Anna Louise","family":"Vestergaard","sequence":"first","affiliation":[{"name":"Department of Obstetrics and Gynecology Randers Regional Hospital Randers Denmark"},{"name":"Department of Biomedicine Pharmacology Aarhus University Aarhus Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1734-7321","authenticated-orcid":false,"given":"Signe","family":"Justesen","sequence":"additional","affiliation":[{"name":"Department of Biomedicine Pharmacology Aarhus University Aarhus Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tabia","family":"Volqvartz","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology Randers Regional Hospital Randers Denmark"},{"name":"Department of Biomedicine Pharmacology Aarhus University Aarhus Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sissel K.","family":"Aagaard","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology Randers Regional Hospital Randers Denmark"},{"name":"Department of Biomedicine Pharmacology Aarhus University Aarhus Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mette F.","family":"Andreasen","sequence":"additional","affiliation":[{"name":"Section for Forensic Chemistry Department of Forensic Medicine Aarhus University Aarhus Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Iana","family":"Lesnikova","sequence":"additional","affiliation":[{"name":"Department of Pathology Rush University Medical Center Chicago IL USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Niels","family":"Uldbjerg","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology Aarhus University Hospital Aarhus Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Agnete","family":"Larsen","sequence":"additional","affiliation":[{"name":"Department of Biomedicine Pharmacology Aarhus University Aarhus Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pinar","family":"Bor","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology Randers Regional Hospital Randers Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2021,1,12]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.3390\/nu10030294"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11154-017-9414-3"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.3109\/14767058.2013.765849"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1093\/nutrit\/nuw033"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.abb.2011.11.018"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.3390\/nu8090533"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.3109\/00016348609158232"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/ejcn.2013.152"},{"key":"e_1_2_10_10_1","unstructured":"DSOG.D\u2010vitamin mangel. 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