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It has been shown that the microbial profile of endometrium could have a role in fertilization and pregnancy outcomes. In this study we aim to assess the microbial community of endometrial tissue (ET) and endometrial fluid (EF) samples in women receiving in vitro fertilization (IVF) treatment. We also search for possible associations between chronic endometritis (CE) and endometrial microbiota.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Material and methods<\/jats:title>\n                    <jats:p>This was a cohort study involving 25 women aged between 28 and 42\u00a0years with both primary and secondary infertility and with at least one IVF failure. The ET and EF sample collection was carried out between September 2016 and November 2018. Each of the participants provided two types of samples\u2014tissue and fluid samples (50\u00a0samples in total). A 16S rRNA sequencing was performed on both of the sample types for microbial profile evaluation. CE was diagnosed based on a CD138 immunohistochemistry where CE diagnosis was confirmed in the presence of one or more plasma cells. Microbial profiles of women with and without CE were compared in both sample types separately.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      We report no differences in the microbial composition and alpha diversity (\n                      <jats:italic>p<\/jats:italic>\n                      <jats:sub>Observed<\/jats:sub>\n                      \u00a0=\u00a00.07,\n                      <jats:italic>p<\/jats:italic>\n                      <jats:sub>Shannon<\/jats:sub>\n                      \u00a0=\u00a00.65,\n                      <jats:italic>p<\/jats:italic>\n                      <jats:sub>Inverse Simpson<\/jats:sub>\n                      \u00a0=\u00a00.59) between the EF and ET samples of IVF patients. We show that the abundance of the genus\n                      <jats:italic>Lactobacillus<\/jats:italic>\n                      influences the variation in microbial beta diversity between and fluid samples (\n                      <jats:italic>r<\/jats:italic>\n                      <jats:sup>2<\/jats:sup>\n                      \u00a0=\u00a00.34; false discovery rate [FDR]\u00a0&lt;9.9\u00a0\u00d7\u00a010\n                      <jats:sup>\u22125<\/jats:sup>\n                      ). We report that 32% (8\/25) of the participants had differences in\n                      <jats:italic>Lactobacillus<\/jats:italic>\n                      dominance in the paired samples and these samples also present a different microbial diversity (\n                      <jats:italic>p<\/jats:italic>\n                      <jats:sub>Shannon<\/jats:sub>\n                      \u00a0=\u00a00.06, FDR\n                      <jats:sub>weighted UniFrac<\/jats:sub>\n                      \u00a0=\u00a00.01). These results suggest that the microbial differences between ET and fluid samples are driven by the abundance of genus\n                      <jats:italic>Lactobacillus<\/jats:italic>\n                      . The microbiome of CE and without CE (ie non\u2010CE) women in our sample set of IVF patients was similar.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>\n                      Our findings show that genus\n                      <jats:italic>Lactobacillus<\/jats:italic>\n                      dominance is an important factor influencing the microbial composition of ET and fluid samples.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/aogs.14297","type":"journal-article","created":{"date-parts":[[2022,1,29]],"date-time":"2022-01-29T00:54:32Z","timestamp":1643417672000},"page":"212-220","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":27,"title":["Differences in microbial profile of endometrial fluid and tissue samples in women with in vitro fertilization failure are driven by\n                    <i>Lactobacillus<\/i>\n                    abundance"],"prefix":"10.1111","volume":"101","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9722-5876","authenticated-orcid":false,"given":"Kreete","family":"L\u00fcll","sequence":"first","affiliation":[{"name":"Estonian Genome Center Institute of Genomics University of Tartu  Tartu Estonia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Merli","family":"Saare","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology Institute of Clinical Medicine University of Tartu  Tartu Estonia"},{"name":"Competence Center on Health Technologies  Tartu Estonia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maire","family":"Peters","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology Institute of Clinical Medicine University of Tartu  Tartu Estonia"},{"name":"Competence Center on Health Technologies  Tartu Estonia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ekaterina","family":"Kakhiani","sequence":"additional","affiliation":[{"name":"Faculty of Pediatrics North\u2010Western State Medical University I.I. Mechnikov  St. Petersburg Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anastasia","family":"Zhdanova","sequence":"additional","affiliation":[{"name":"Medical Faculty The Pavlov First Saint\u2010Petersburg State Medical University  St. Petersburg Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andres","family":"Salumets","sequence":"additional","affiliation":[{"name":"Estonian Genome Center Institute of Genomics University of Tartu  Tartu Estonia"},{"name":"Department of Obstetrics and Gynecology Institute of Clinical Medicine University of Tartu  Tartu Estonia"},{"name":"Competence Center on Health Technologies  Tartu Estonia"},{"name":"Division of Obstetrics and Gynecology Department of Clinical Science, Intervention and Technology (CLINTEC) Karolinska Institutet  Stockholm Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Konstantin","family":"Boyarsky","sequence":"additional","affiliation":[{"name":"Center of 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