{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,12]],"date-time":"2026-05-12T15:36:53Z","timestamp":1778600213748,"version":"3.51.4"},"reference-count":31,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2022,6,11]],"date-time":"2022-06-11T00:00:00Z","timestamp":1654905600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["obgyn.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Acta Obstet Gynecol Scand"],"published-print":{"date-parts":[[2022,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Introduction<\/jats:title><jats:p>Treatment with antibodies directed against programed\u2010cell death ligand 1 (PD\u2010L1) is a novel therapy for patients with gestational trophoblastic disease. Assessment of PD\u2010L1 expression in tumor tissue is commonly used to identify patients who might benefit from anti\u2010PD\u2010L1 treatment. Multiple antibodies are available to detect PD\u2010L1\u2010expressing cells, and percentages of PD\u2010L1\u2010expressing cells in samples of patients with gestational trophoblastic disease indicated by these antibodies differ substantially. This raises the question which PD\u2010L1 antibody best reflects PD\u2010L1 expression to select patients for treatment.<\/jats:p><\/jats:sec><jats:sec><jats:title>Material and methods<\/jats:title><jats:p>Seven commercially available antibodies for PD\u2010L1 staining (E1L3N, 73\u201310, 22C3, CAL10, SP142, 28\u20138, SP263) were validated on Chinese hamster ovarian (CHO) cells transfected with PD\u2010L1, PD\u2010L2, wildtype CHO cells and tonsil tissue. Next, four complete hydatidiform moles and four choriocarcinomas were stained. Samples were independently assessed by two pathologists.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>All seven antibodies showed membranous staining in the PD\u2010L1\u2010transfected CHO cells. E1L3N and 22C3 scored the highest percentages of PD\u2010L1\u2010positive cells (70%\u201390% and 60%\u201370%, respectively). E1L3N stained the cytoplasm of non\u2010transfected CHO cells and was excluded from analysis. The remaining six antibodies predominantly stained syncytiotrophoblast cells of both complete hydatidiform moles and choriocarcinomas. The percentage of PD\u2010L1\u2010stained trophoblast cells and staining intensity varied substantially per used PD\u2010L1 antibody and between complete hydatidiform moles and choriocarcinomas. Agreement between pathologists was best with 22C3 (intraclass correlation coefficient 0.94\u20130.96).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Based on staining results of the CHO cells, gestational trophoblastic disease samples and intraclass correlation coefficient, 22C3 seems the most suitable for adequate detection of PD\u2010L1\u2010expressing trophoblast cells. All antibodies detected PD\u2010L1\u2010expressing cells in the gestational trophoblastic disease samples, though with great variability, hampering comparison of results between studies in this rare disease and emphasizing the need for uniformity in detecting PD\u2010L1\u2010expressing cells.<\/jats:p><\/jats:sec>","DOI":"10.1111\/aogs.14404","type":"journal-article","created":{"date-parts":[[2022,6,11]],"date-time":"2022-06-11T04:49:55Z","timestamp":1654922995000},"page":"1007-1016","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["<scp>PD\u2010L1<\/scp> in gestational trophoblastic disease: an antibody evaluation"],"prefix":"10.1111","volume":"101","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3327-9218","authenticated-orcid":false,"given":"Yvonne M.","family":"Hoeijmakers","sequence":"first","affiliation":[{"name":"Department of Obstetrics and Gynecology Radboud University Medical Center  Nijmegen The Netherlands"},{"name":"Department of Laboratory Medicine Radboud University Medical Center  Nijmegen The Netherlands"},{"name":"Department of Medical Oncology Radboud University Medical Center  Nijmegen The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michiel","family":"Simons","sequence":"additional","affiliation":[{"name":"Department of Pathology Radboud University Medical Center  Nijmegen The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Johan","family":"Bulten","sequence":"additional","affiliation":[{"name":"Department of Pathology Radboud University Medical Center  Nijmegen The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mark A. J.","family":"Gorris","sequence":"additional","affiliation":[{"name":"Department of Tumor Immunology Radboud Institute for Molecular Life Sciences, Radboud University Medical Center  Nijmegen The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Petronella B.","family":"Ottevanger","sequence":"additional","affiliation":[{"name":"Department of Medical Oncology Radboud University Medical Center  Nijmegen The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"I. Jolanda M.","family":"de Vries","sequence":"additional","affiliation":[{"name":"Department of Tumor Immunology Radboud Institute for Molecular Life Sciences, Radboud University Medical Center  Nijmegen The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fred C. G. 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