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Vaginal secretions from the posterior fornix of women in the early stages of pregnancy were collected in the Xiangya Third Hospital of Central South University in Changsha, Hunan, China, from November 2018 to November 2019. A total of 54 cases of missed abortion (case group) and 50 cases of normal pregnancy requiring induced abortion (reference group) were analyzed. Bacterial DNA was extracted, hybridized with gene\u2010specific primers, and then detected using a bacterial chip. The composition and relative abundance of vaginal microbiota in the two groups were compared using \u03b1\u2010diversity analysis, \u03b2\u2010diversity analysis, and the linear discriminant analysis effect size method.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The \u03b1\u2010diversity analysis showed that the Simpson index of the case group was lower than that of the reference group, whereas the Shannon index in the case group was higher. The relative abundance of <jats:italic>Firmicutes<\/jats:italic> in the case group (42.52%) was lower than in the reference group (51.03%, <jats:italic>p<\/jats:italic>\u2009&lt;\u20090.05), as was the relative abundance of <jats:italic>Lactobacillus<\/jats:italic> (case group 16.51%, reference group 23.00%; <jats:italic>p<\/jats:italic>\u2009&lt;\u20090.05). Interestingly, levels of <jats:italic>Mycoplasma genitalium<\/jats:italic> and <jats:italic>Ureaplasma<\/jats:italic> were lower in the case group (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.05). The relative abundance of <jats:italic>Lactobacillus crispatus<\/jats:italic>, <jats:italic>Lactobacillus jensenii<\/jats:italic>, and <jats:italic>Lactobacillus gasseri<\/jats:italic> was also significantly lower in the case group than in the reference group (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.05). The pathways enriched in the case group were predominantly related to metabolism, whereas the genetics\u2010related pathways were predominantly enriched in the reference group.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Bacteria are more diverse and unevenly distributed in patients with missed abortion. Decreases in the proportion of vaginal <jats:italic>Lactobacillus<\/jats:italic> and changes in <jats:italic>Lactobacillus<\/jats:italic> species in these patients may increase the chance of genital tract pathogenic bacterial infection. To our knowledge, our study was the first to observe that a decrease of <jats:italic>Firmicutes<\/jats:italic> levels in the vaginal microbiota might impair energy metabolism and have an association with missed abortion.<\/jats:p><\/jats:sec>","DOI":"10.1111\/aogs.14410","type":"journal-article","created":{"date-parts":[[2022,7,25]],"date-time":"2022-07-25T00:53:52Z","timestamp":1658710432000},"page":"960-971","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":22,"title":["The association between vaginal microbiota disorders and early missed abortion: A prospective study"],"prefix":"10.1111","volume":"101","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8547-2749","authenticated-orcid":false,"given":"Dan","family":"Sun","sequence":"first","affiliation":[{"name":"Department of Obstetrics and Gynecology Third Xiangya Hospital of Central South University  Changsha Hunan 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