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The gold standard for the diagnosis of intra\u2010amniotic inflammation is the determination of an amniotic fluid profile obtained from transabdominal amniocentesis, which is invasive. Cervicovaginal fluid fetal fibronectin (fFN) is a widely\u2010used predictive biomarker for spontaneous preterm labor. The aims of this study are to determine (1) whether a quantitative cervicovaginal fluid fFN test can be used to identify the presence of intra\u2010amniotic inflammation; and (2) an appropriate cut\u2010off value of a cervicovaginal fluid fFN concentration for the identification of intra\u2010amniotic inflammation.<\/jats:p><\/jats:sec><jats:sec><jats:title>Material and Methods<\/jats:title><jats:p>This prospective cohort study included 78 patients with preterm labor and intact membranes who had a sample collected for quantitative cervicovaginal fluid fFN measurement and underwent transabdominal amniocentesis. Intra\u2010amniotic inflammation was defined as an amniotic fluid interleukin\u20106 concentration\u2009\u22652.6\u2009ng\/mL. Clinicians were masked from the results of cervicovaginal fluid fFN and amniotic fluid interleukin\u20106 concentrations. Logistic regression analysis was used to determine which factors were significant predictors of intra\u2010amniotic inflammation. The diagnostic indices of the cervicovaginal fluid fFN test for the identification of intra\u2010amniotic inflammation were calculated.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>(1) Frequency of intra\u2010amniotic inflammation was 26.9% (21\/78); (2) the higher the cervicovaginal fluid fFN concentration, the greater the risk of intra\u2010amniotic inflammation (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.001); (3) cervicovaginal fluid fFN concentration\u2009\u2265125\u2009ng\/mL had an area under the curve of 0.91 (95% confidence interval: 0.83\u20130.96) for the identification of intra\u2010amniotic inflammation with 100% sensitivity, 100% negative predictive value, 82.46% specificity and a positive likelihood ratio of 5.7; and (4) cervicovaginal fluid fFN cut\u2010off of 125\u2009ng\/mL had a significant higher predictive performance than the traditional cut\u2010off (50\u2009ng\/mL) for the identification of intra\u2010amniotic inflammation.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Quantitative cervicovaginal fluid fFN with a cut\u2010off of 125\u2009ng\/mL had a high sensitivity and a negative predictive value as well as a positive likelihood ratio for the identification of intra\u2010amniotic inflammation. Its high sensitivity and negative predictive value can be used to decrease an index of suspicion of intra\u2010amniotic inflammation. This test may be useful as an initial assessment test to select appropriate patients for amniocentesis to determine intra\u2010amniotic inflammation.<\/jats:p><\/jats:sec>","DOI":"10.1111\/aogs.14899","type":"journal-article","created":{"date-parts":[[2024,9,17]],"date-time":"2024-09-17T14:40:23Z","timestamp":1726584023000},"page":"2252-2263","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Quantitative cervicovaginal fluid fetal fibronectin: A liquid biopsy for intra\u2010amniotic inflammation"],"prefix":"10.1111","volume":"103","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7946-3774","authenticated-orcid":false,"given":"Puntabut","family":"Warintaksa","sequence":"first","affiliation":[{"name":"Department of Obstetrics and Gynecology, Faculty of Medicine, Ramathibodi Hospital Mahidol University  Bangkok 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