{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,14]],"date-time":"2025-05-14T00:41:25Z","timestamp":1747183285097,"version":"3.40.5"},"reference-count":18,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2024,10,14]],"date-time":"2024-10-14T00:00:00Z","timestamp":1728864000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","award":["2017\/05389\u20104"],"award-info":[{"award-number":["2017\/05389\u20104"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["obgyn.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Acta Obstet Gynecol Scand"],"published-print":{"date-parts":[[2025,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Introduction<\/jats:title><jats:p>Uterine transplantation was developed for the treatment of absolute uterine factor infertility. As it is a new modality of transplantation, there is still room for technical improvement. A factor that impacts graft survival in organ transplantation is the warm ischemia time. In uterine transplantation specifically, at least two vascular anastomoses are performed on each side of the uterus, and the graft revascularization takes place when the vascular clamps of the arteries and veins are released on both sides simultaneously. For this reason, the warm ischemia time in uterine transplant is expected to be considerably long. The purpose of this study was to compare the sequential technique of uterine graft revascularization, which aims to reduce the warm ischemia time of the procedure, with the simultaneous revascularization technique.<\/jats:p><\/jats:sec><jats:sec><jats:title>Material and Methods<\/jats:title><jats:p>For the procedure, the uterine auto\u2010transplantation technique was performed using 10 non\u2010pregnant adult ewes weighing about 45\u2009kg, divided into two groups: simultaneous revascularization group (5 animals) and sequential revascularization group (5 animals). To evaluate the groups, we analyzed the procedure and warm ischemia times, graft macroscopy, hemodynamic, laboratory, and histological parameters of the uterus.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The sequential revascularization technique group had similar surgical procedure times, and the warm ischemia time was significantly shorter with medians of 32\u2009min in the sequential group vs 72\u2009min in the simultaneous group (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.008). The graft macroscopy and hemodynamic, laboratory, and histological parameters evaluated were similar between the groups.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The sequential revascularization technique proved to reduce the warm ischemia time in the sheep uterine auto\u2010transplantation model without compromising graft viability.<\/jats:p><\/jats:sec>","DOI":"10.1111\/aogs.14979","type":"journal-article","created":{"date-parts":[[2024,10,15]],"date-time":"2024-10-15T04:02:50Z","timestamp":1728964970000},"page":"474-482","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Analysis of two reperfusion techniques in uterine transplantation in an experimental model"],"prefix":"10.1111","volume":"104","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5505-6480","authenticated-orcid":false,"given":"Rubens","family":"Macedo Arantes","sequence":"first","affiliation":[{"name":"Department of Gastroenterology, Transplantation Unit Hospital das Cl\u00ednicas da Faculadade de Medicina da Universidade de S\u00e3o Paulo  S\u00e3o Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dani","family":"Ejzenberg","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology Hospital das Cl\u00ednicas da Faculadade de Medicina da Universidade de S\u00e3o Paulo  S\u00e3o Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2357-558X","authenticated-orcid":false,"given":"Ryan Yukimatsu","family":"Tanigawa","sequence":"additional","affiliation":[{"name":"Department of Pathology Hospital das Cl\u00ednicas da Faculadade de Medicina da Universidade de S\u00e3o Paulo  S\u00e3o Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Amadeu Batista","family":"da Silva Neto","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology, Transplantation Unit Hospital das Cl\u00ednicas da Faculadade de Medicina da Universidade de S\u00e3o Paulo  S\u00e3o Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rodrigo Bronze","family":"de Martino","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology, Transplantation Unit Hospital das Cl\u00ednicas da Faculadade de Medicina da Universidade de S\u00e3o Paulo  S\u00e3o Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fl\u00e1vio Henrique","family":"Galv\u00e3o","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology, Transplantation Unit Hospital das Cl\u00ednicas da Faculadade de Medicina da Universidade de S\u00e3o Paulo  S\u00e3o Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4284-0633","authenticated-orcid":false,"given":"Daniel Reis","family":"Waisberg","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology, Transplantation Unit Hospital das Cl\u00ednicas da Faculadade de Medicina da Universidade de S\u00e3o Paulo  S\u00e3o Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Liliana","family":"Ducatti","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology, Transplantation Unit Hospital das Cl\u00ednicas da Faculadade de Medicina da Universidade de S\u00e3o Paulo  S\u00e3o Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vinicius","family":"Rocha Santos","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology, Transplantation Unit Hospital das Cl\u00ednicas da Faculadade de Medicina da Universidade de S\u00e3o Paulo  S\u00e3o Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rafael Nunes","family":"Pinheiro","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology, Transplantation Unit Hospital das Cl\u00ednicas da Faculadade de Medicina da Universidade de S\u00e3o Paulo  S\u00e3o Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luciana Bertocco","family":"Haddad","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology, Transplantation Unit Hospital das Cl\u00ednicas da Faculadade de Medicina da Universidade de S\u00e3o Paulo  S\u00e3o Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andr\u00e9 Dong","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology, Transplantation Unit Hospital das Cl\u00ednicas da Faculadade de Medicina da Universidade de S\u00e3o Paulo  S\u00e3o Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jos\u00e9 Maria","family":"Soares\u2010Junior","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology Hospital das Cl\u00ednicas da Faculadade de Medicina da Universidade de S\u00e3o Paulo  S\u00e3o Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Edmund Chada","family":"Baracat","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology Hospital das Cl\u00ednicas da Faculadade de Medicina da Universidade de S\u00e3o Paulo  S\u00e3o Paulo Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luiz Augusto","family":"Carneiro D'Albuquerque","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology, Transplantation Unit Hospital das Cl\u00ednicas da Faculadade de Medicina da Universidade de S\u00e3o Paulo  S\u00e3o Paulo 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