{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,4]],"date-time":"2026-09-04T06:07:51Z","timestamp":1788502071600,"version":"build-2803163510"},"reference-count":40,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2024,12,6]],"date-time":"2024-12-06T00:00:00Z","timestamp":1733443200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004063","name":"Knut och Alice Wallenbergs Stiftelse","doi-asserted-by":"publisher","award":["2017.0363"],"award-info":[{"award-number":["2017.0363"]}],"id":[{"id":"10.13039\/501100004063","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004359","name":"Vetenskapsr\u00e5det","doi-asserted-by":"publisher","award":["2024\u201003487"],"award-info":[{"award-number":["2024\u201003487"]}],"id":[{"id":"10.13039\/501100004359","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["obgyn.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Acta Obstet Gynecol Scand"],"published-print":{"date-parts":[[2025,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Introduction<\/jats:title><jats:p>Uterus bioengineering offers a potential treatment option for women with uterine factor infertility and for mitigating the risk of uterine rupture associated with women with defective uterine tissue. Decellularized uterine tissue scaffolds proved promising in further in\u00a0vivo experiments in rodent and domestic species animal models. Variations in the extracellular matrix composition among different species and adaptations of the decellularization protocols make it difficult to compare the results between studies. Therefore, we assessed if our earlier developed sodium deoxycholate\u2010based decellularization protocol for the sheep and the cow uterus could become a standardized cross\u2010species protocol by assessing it on the non\u2010human primate (baboon) uterus.<\/jats:p><\/jats:sec><jats:sec><jats:title>Material and Methods<\/jats:title><jats:p>The baboon uterus was decellularized using sodium deoxycholate, and the remaining acellular scaffold was quantitatively assessed for DNA, protein, and specific extracellular matrix components. Furthermore, electron microscopy deepened morphology examination, while the chorioallantoic membrane assay examined the scaffolds' cytotoxicity, bioactivity, and angiogenic properties. The in\u00a0vitro recellularization efficiency of the scaffolds using xenogeneic (rat) bone marrow\u2010derived mesenchymal stem cells was also assessed. Finally, the immune potential of the scaffolds was evaluated by in\u00a0vitro exposure to human peripheral blood mononuclear cells.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>We obtained a decellularized baboon uterus with preserved extracellular matrix components by adding an 8\u2010h sodium deoxycholate perfusion to our previously developed protocol for the sheep and cow models. This minor modification resulted in scaffolds with less than 1% of immunogenic host DNA content while preserving important uterine\u2010specific collagen, elastin, and glycosaminoglycan structures. The chorioallantoic membrane assay and in\u00a0vitro recellularization experiments confirmed that the scaffolds were bioactive and non\u2010cytotoxic. As we have observed in other animal models, the enzymatic scaffold preconditioning with matrix metalloproteinases improved the recellularization efficiency further. Additionally, the preconditioning generated more immune\u2010privileged scaffolds, as shown in a novel in\u00a0vitro co\u2010culture assay with human peripheral blood mononuclear cells.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>For the first time, our data demonstrate the efficiency of our protocol for non\u2010human primate uteri and its translational potential. This standardized protocol will facilitate cross\u2010study comparisons and expedite clinical translation.<\/jats:p><\/jats:sec>","DOI":"10.1111\/aogs.15030","type":"journal-article","created":{"date-parts":[[2024,12,6]],"date-time":"2024-12-06T14:00:55Z","timestamp":1733493655000},"page":"483-493","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Toward human uterus tissue engineering: Uterine decellularization in a non\u2010human primate species"],"prefix":"10.1111","volume":"104","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6556-9108","authenticated-orcid":false,"given":"Luc\u00eda","family":"De Miguel\u2010G\u00f3mez","sequence":"first","affiliation":[{"name":"Laboratory for Transplantation and Regenerative Medicine, Sahlgrenska Academy University of Gothenburg  Gothenburg Sweden"},{"name":"Department of Obstetrics and Gynecology, Clinical Sciences, Sahlgrenska Academy University of Gothenburg  Gothenburg 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