{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,7,18]],"date-time":"2024-07-18T23:08:50Z","timestamp":1721344130603},"reference-count":38,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2013,10,11]],"date-time":"2013-10-11T00:00:00Z","timestamp":1381449600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Artificial Organs"],"published-print":{"date-parts":[[2014,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The effect of adding pulsatility to gaseous oxygen persufflation during liver preservation was studied in an isolated rat liver model. Livers from male <jats:styled-content style=\"fixed-case\">W<\/jats:styled-content>istar rats were retrieved 30\u2009min after cardiac arrest of the donor and subjected to 18\u2009h of cold storage. Some grafts were subjected to nonpulsatile or pulsatile gaseous oxygen persufflation. Graft viability was assessed thereafter upon warm reperfusion in vitro (<jats:italic>n<\/jats:italic>\u2009=\u20095 per group). Pulsatile persufflation significantly improved parenchymal integrity (enzyme release, bile flow) upon reperfusion, with respect to nonpulsatile persufflation or cold storage (<jats:styled-content style=\"fixed-case\">CS<\/jats:styled-content>) (e.g., max. release of alanine aminotransferase: 44\u2009\u00b1\u200910 vs. 178\u2009\u00b1\u200929 vs. 345\u2009\u00b1\u2009100\u2009<jats:styled-content style=\"fixed-case\">U<\/jats:styled-content>\/<jats:styled-content style=\"fixed-case\">L<\/jats:styled-content>; pulsatile vs. nonpulsatile persufflation vs. <jats:styled-content style=\"fixed-case\">CS<\/jats:styled-content>).The effect was associated with the prevention of the ischemic decline in gene and protein expression of the vasoprotective <jats:styled-content style=\"fixed-case\">K<\/jats:styled-content>r\u00fcppel\u2010like factor 2, increased perfusate levels of the endogenous vasodilator nitric oxide, and reduced portal vascular resistance upon reperfusion, while nonpulsatile persufflation was less effective (e.g., vascular resistance: 1235\u2009\u00b1\u2009108 vs. 1607\u2009\u00b1\u2009155 vs. 2215\u2009\u00b1\u2009208\u2009<jats:styled-content style=\"fixed-case\">P<\/jats:styled-content>a s\/mL; pulsatile vs. nonpulsatile persufflation vs. <jats:styled-content style=\"fixed-case\">CS<\/jats:styled-content>). In conclusion, pulsatile mechanostimulation of the hepatovasculature seems a genuine protective mechanism, affecting early graft recovery upon reperfusion.<\/jats:p>","DOI":"10.1111\/aor.12184","type":"journal-article","created":{"date-parts":[[2013,10,14]],"date-time":"2013-10-14T11:54:12Z","timestamp":1381751652000},"page":"404-410","source":"Crossref","is-referenced-by-count":11,"title":["Adding Pulsatile Vascular Stimulation to Venous Systemic <scp>O<\/scp>xygen Persufflation of Liver Grafts"],"prefix":"10.1111","volume":"38","author":[{"given":"Bastian","family":"L\u00fcer","sequence":"first","affiliation":[{"name":"Surgical Research Division University Clinic of Surgery  Bonn Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mario","family":"Fox","sequence":"additional","affiliation":[{"name":"Surgical Research Division University Clinic of Surgery  Bonn Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Patrik","family":"Efferz","sequence":"additional","affiliation":[{"name":"Surgical Research Division University Clinic of Surgery  Bonn Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thomas","family":"Minor","sequence":"additional","affiliation":[{"name":"Surgical Research Division University Clinic of Surgery  Bonn Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2013,10,11]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-6143.2009.02932.x"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1097\/TP.0b013e3181995e83"},{"key":"e_1_2_6_4_1","first-page":"125","volume-title":"Organ Preservation and Reengineering","author":"Minor 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