{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T10:53:28Z","timestamp":1792666408496,"version":"4.5.0"},"reference-count":41,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2014,4,9]],"date-time":"2014-04-09T00:00:00Z","timestamp":1397001600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/3.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Artificial Organs"],"published-print":{"date-parts":[[2014,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The hemodialysis procedure involves contact between peripheral blood and the surface of dialyzer membranes, which may lead to alterations in the pathways of innate and adaptive immunity. We aimed to study the effect of blood\u2013membrane interaction on human peripheral basophils and neutrophils in hemodialysis with high\u2010 and low\u2010permeability polysulfone dialyzers. The surface expression of <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>203c (basophil selection marker) and <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>63 (activation marker) after activation by the bacterial peptide formyl\u2010methionyl\u2010leucyl\u2010phenylalanine (<jats:styled-content style=\"fixed-case\">fMLP<\/jats:styled-content>) or anti\u2010<jats:styled-content style=\"fixed-case\">F<\/jats:styled-content>c\u03b5 receptor I (<jats:styled-content style=\"fixed-case\">F<\/jats:styled-content>c\u03b5<jats:styled-content style=\"fixed-case\">RI<\/jats:styled-content>) antibody and the absolute number of basophils was investigated before and after hemodialysis with each of the dialyzers. Moreover, the expression on neutrophils of <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>11b, the <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>11b active epitope, and <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>88 was analyzed in the same groups of individuals. The expression of <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>63 in basophils following activation by <jats:styled-content style=\"fixed-case\">fMLP<\/jats:styled-content> was significantly higher in the patient group compared with that in healthy controls, but no differences were observed after activation by anti\u2010<jats:styled-content style=\"fixed-case\">F<\/jats:styled-content>c\u03b5<jats:styled-content style=\"fixed-case\">RI<\/jats:styled-content>. During the hemodialysis procedure, the low\u2010flux membrane induced up\u2010regulation of <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>63 expression on basophils, while passage through the high\u2010flux membrane did not significantly alter the responsiveness. In addition, the absolute number of basophils was unchanged after hemodialysis with either of the dialyzers and compared with healthy controls. We found no significant differences in the expression of the neutrophil activation markers (<jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>11b, the active epitope of <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>11b, and <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>88) comparing the two different dialyzers before and after dialysis and healthy controls. Together, these findings suggest that alterations in basophil activity may be a useful marker of membrane bioincompatibility in hemodialysis.<\/jats:p>","DOI":"10.1111\/aor.12297","type":"journal-article","created":{"date-parts":[[2014,4,9]],"date-time":"2014-04-09T09:02:10Z","timestamp":1397034130000},"page":"945-953","source":"Crossref","is-referenced-by-count":13,"title":["Activation of Basophils Is a New and Sensitive Marker of Biocompatibility in Hemodialysis"],"prefix":"10.1111","volume":"38","author":[{"given":"Zenib","family":"Aljadi","sequence":"first","affiliation":[{"name":"Unit of Clinical Immunology and Allergy Department of Medicine Karolinska University Hospital Solna Karolinska Institute  Stockholm Sweden"},{"name":"Division of Proteomics and Nanobiotechnology School of Biotechnology Royal Institute of Technology  Stockholm 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