{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,6]],"date-time":"2025-10-06T19:19:38Z","timestamp":1759778378321},"reference-count":34,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2014,5,20]],"date-time":"2014-05-20T00:00:00Z","timestamp":1400544000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Artificial Organs"],"published-print":{"date-parts":[[2014,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Tissue engineering of cardiovascular structures represents a novel approach to improve clinical strategies in heart valve disease treatment. The aim of this study was to engineer decellularized atrioventricular heart valve neoscaffolds with an intact ultrastructure and to reseed them with umbilical cord\u2010derived endothelial cells under physiological conditions in a bioreactor environment. Mitral (<jats:italic>n<\/jats:italic>\u2009=\u200938) and tricuspid (<jats:italic>n<\/jats:italic>\u2009=\u200936) valves were harvested from 40 hearts of <jats:styled-content style=\"fixed-case\">G<\/jats:styled-content>erman <jats:styled-content style=\"fixed-case\">L<\/jats:styled-content>andrace swine from a selected abattoir. Decellularization of atrioventricular heart valves was achieved by a detergent\u2010based cell extraction protocol. Evaluation of the decellularization method was conducted with light microscopy and quantitative analysis of collagen and elastin content. The presence of residual <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> within the decellularized atrioventricular heart valves was determined with spectrophotometric quantification. The described decellularization regime produced full removal of native cells while maintaining the mechanical stability and the quantitative composition of the atrioventricular heart valve neoscaffolds. The surface of the xenogeneic matrix could be successfully reseeded with in vitro\u2010expanded human umbilical cord\u2010derived endothelial cells under physiological flow conditions. After complete decellularization with the detergent\u2010based protocol described here, physiological reseeding of the xenogeneic neoscaffolds resulted in the formation of a confluent layer of human umbilical cord\u2010derived endothelial cells. These results warrant further research toward the generation of atrioventricular heart valve neoscaffolds on the basis of decellularized xenogeneic tissue.<\/jats:p>","DOI":"10.1111\/aor.12321","type":"journal-article","created":{"date-parts":[[2014,5,20]],"date-time":"2014-05-20T05:03:22Z","timestamp":1400562202000},"source":"Crossref","is-referenced-by-count":10,"title":["In Vitro Generation of Atrioventricular Heart Valve Neoscaffolds"],"prefix":"10.1111","volume":"38","author":[{"given":"Alexander","family":"Weymann","sequence":"first","affiliation":[{"name":"Heart and Marfan Center Department of Cardiac Surgery University of Heidelberg  Heidelberg Germany"},{"name":"Department of Cardiothoracic Transplantation and Mechanical Circulatory Support Royal Brompton &amp; Harefield NHS Foundation Trust  Harefield Middlesex UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tam\u00e1s","family":"Radovits","sequence":"additional","affiliation":[{"name":"Heart and Marfan 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