{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,13]],"date-time":"2026-01-13T16:58:03Z","timestamp":1768323483948,"version":"3.49.0"},"reference-count":20,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2015,4,20]],"date-time":"2015-04-20T00:00:00Z","timestamp":1429488000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Land Baden-W\u00fcrttemberg, Germany, the Medical Faculty of the University of Heidelberg, Germany"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Artificial Organs"],"published-print":{"date-parts":[[2015,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Decellularization of native organs may provide an acellular tissue platform for organ regeneration. However, decellularization involves a trade\u2010off between removal of immunogenic cellular elements and preservation of biomechanical integrity. We sought to develop a bioartificial scaffold for respiratory tissue engineering by decellularization of porcine lungs and trachea while preserving organ architecture and vasculature. Lung\u2013trachea preparations from 25 <jats:styled-content style=\"fixed-case\">G<\/jats:styled-content>erman <jats:styled-content style=\"fixed-case\">L<\/jats:styled-content>andrace pigs were perfused in a modified Langendorff circuit and decellularized by an <jats:styled-content style=\"fixed-case\">SDC<\/jats:styled-content> (sodium deoxycholate)\u2010based perfusion protocol. Decellularization was evaluated by histology and fluorescence microscopy, and residual <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> quantified spectrophotometrically and compared with controls. Airway compliance was evaluated by endotracheal intubation and mechanical ventilation to simulate physiological breathing\u2010induced stretch. Structural integrity was evaluated by bronchoscopy and biomechanical stress\/strain analysis by measuring passive tensile strength, all compared with controls. Decellularized lungs and trachea lacked intracellular components but retained specific collagen fibers and elastin. Quantitative <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> analysis demonstrated a significant reduction of <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> compared with controls (32.8\u2009\u00b1\u200912.4\u2009\u03bcg <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content>\/mg tissue vs. 179.7\u2009\u00b1\u200935.8\u2009\u03bcg <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content>\/mg tissue, <jats:italic>P<\/jats:italic>\u2009&lt;\u20090.05). Lungs and trachea decellularized by our perfusion protocol demonstrated increased airway compliance but preserved biomechanical integrity as compared with native tissue. Whole porcine lungs\u2013tracheae can be successfully decellularized to create an acellular scaffold that preserves extracellular matrix and retains structral integrity and three\u2010dimensional architecture to provide a bioartifical platform for respiratory tissue engineering.<\/jats:p>","DOI":"10.1111\/aor.12481","type":"journal-article","created":{"date-parts":[[2015,4,20]],"date-time":"2015-04-20T11:43:02Z","timestamp":1429530182000},"page":"1024-1032","source":"Crossref","is-referenced-by-count":41,"title":["Perfusion\u2010Decellularization of Porcine Lung and Trachea for Respiratory Bioengineering"],"prefix":"10.1111","volume":"39","author":[{"given":"Alexander","family":"Weymann","sequence":"first","affiliation":[{"name":"Department of Cardiac Surgery Heart and Marfan Center, University of Heidelberg  Heidelberg Germany"},{"name":"Department of Cardiothoracic Transplantation &amp; Mechanical Circulatory Support Royal Brompton and Harefield NHS Foundation Trust  Middlesex UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nikhil Prakash","family":"Patil","sequence":"additional","affiliation":[{"name":"Department of Cardiothoracic Transplantation &amp; Mechanical Circulatory Support Royal Brompton and Harefield NHS Foundation Trust  Middlesex UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anton","family":"Sabashnikov","sequence":"additional","affiliation":[{"name":"Department of Cardiothoracic Transplantation &amp; Mechanical Circulatory Support Royal Brompton and Harefield NHS Foundation Trust  Middlesex UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sevil","family":"Korkmaz","sequence":"additional","affiliation":[{"name":"Department of Cardiac Surgery Heart and Marfan Center, University of Heidelberg  Heidelberg Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shiliang","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Cardiac Surgery Heart and Marfan Center, University of Heidelberg  Heidelberg Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pal","family":"Soos","sequence":"additional","affiliation":[{"name":"Heart and Vascular Center Semmelweis University  Budapest Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Roland","family":"Ishtok","sequence":"additional","affiliation":[{"name":"2nd Department of Pathology Semmelweis University  Budapest Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nicole","family":"Chaimow","sequence":"additional","affiliation":[{"name":"Department of Cardiac Surgery Heart and Marfan Center, University of Heidelberg  Heidelberg Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ines","family":"P\u00e4tzold","sequence":"additional","affiliation":[{"name":"Department of Cardiac Surgery Heart and Marfan Center, University of Heidelberg  Heidelberg Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Natalie","family":"Czerny","sequence":"additional","affiliation":[{"name":"Department of Cardiac Surgery Heart and Marfan Center, University of Heidelberg  Heidelberg Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bastian","family":"Schmack","sequence":"additional","affiliation":[{"name":"Department of Cardiac Surgery Heart and Marfan Center, University of Heidelberg  Heidelberg Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aron\u2010Frederik","family":"Popov","sequence":"additional","affiliation":[{"name":"Department of Cardiothoracic Transplantation &amp; Mechanical Circulatory Support Royal Brompton and Harefield NHS Foundation Trust  Middlesex UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andre R\u00fcdiger","family":"Simon","sequence":"additional","affiliation":[{"name":"Department of Cardiothoracic Transplantation &amp; Mechanical Circulatory Support Royal Brompton and Harefield NHS Foundation Trust  Middlesex UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Matthias","family":"Karck","sequence":"additional","affiliation":[{"name":"Department of Cardiac Surgery Heart and Marfan Center, University of Heidelberg  Heidelberg Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gabor","family":"Szabo","sequence":"additional","affiliation":[{"name":"Department of Cardiac Surgery Heart and Marfan Center, University of Heidelberg  Heidelberg Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2015,4,20]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncpcardio0510"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1101\/cshperspect.a015636"},{"key":"e_1_2_9_4_1","first-page":"279","article-title":"Clinical experience with expanded use of the Ross procedure: a paradigm shift?","volume":"19","author":"Weymann A","year":"2010","journal-title":"J Heart Valve Dis"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI61974"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molmed.2011.03.005"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1089\/ten.tec.2011.0567"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.athoracsur.2011.05.018"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1189345"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1097\/MOT.0000000000000051"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1089\/ten.tea.2009.0659"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/aor.12321"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1253\/circj.CJ-10-0717"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.12659\/MSMBR.890444"},{"issue":"12","key":"e_1_2_9_15_1","first-page":"I63","article-title":"Construction of autologous human heart valves based on an acellular allograft matrix","volume":"106","author":"Cebotari S","year":"2002","journal-title":"Circulation"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1089\/ten.tea.2012.0250"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.athoracsur.2013.04.022"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-2277.2008.00736.x"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCULATIONAHA.111.056952"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2013.11.078"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1089\/ten.tec.2013.0756"}],"container-title":["Artificial Organs"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Faor.12481","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/aor.12481","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,4]],"date-time":"2023-10-04T22:33:56Z","timestamp":1696458836000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/aor.12481"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,4,20]]},"references-count":20,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2015,12]]}},"alternative-id":["10.1111\/aor.12481"],"URL":"https:\/\/doi.org\/10.1111\/aor.12481","archive":["Portico"],"relation":{},"ISSN":["0160-564X","1525-1594"],"issn-type":[{"value":"0160-564X","type":"print"},{"value":"1525-1594","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,4,20]]}}}