{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,11]],"date-time":"2026-04-11T02:23:01Z","timestamp":1775874181559,"version":"3.50.1"},"reference-count":35,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2015,10,30]],"date-time":"2015-10-30T00:00:00Z","timestamp":1446163200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Artificial Organs"],"published-print":{"date-parts":[[2016,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Cartilage defects resulting from osteoarthritis (<jats:styled-content style=\"fixed-case\">OA<\/jats:styled-content>) or physical injury can severely reduce the quality of life for sufferers. Current treatment options are costly and not always effective in producing stable hyaline cartilage. Here we investigated a new treatment option that could potentially repair and regenerate damaged cartilage tissue. This novel approach involves the application of infrapatellar fat\u2010pad derived chondroprogenitor cells onto a mechanically stable biodegradable polymer film that can be easily implanted into a defect site. Poly\u2010\u03b5\u2010caprolactone (<jats:styled-content style=\"fixed-case\">PCL<\/jats:styled-content>) films were fabricated via solvent casting in either acetone or chloroform. The hydrophobicity, mechanical properties, and surface morphology of the films were examined. Progenitor cells from infrapatellar fat\u2010pad were isolated, expanded, and then seeded onto the films. The cells were allowed to self\u2010assemble on films, and these were then cultured in a chemically defined chondrogenic media for 28 days. The self\u2010assembled tissue was characterized via histological staining, gene expression analysis, immunohistochemistry, and biochemical analysis. Chondrogenic differentiation was induced to generate a cartilaginous matrix upon the films. Despite differences between in the appearance, surface morphology, and mechanical properties of the films cast in chloroform or acetone, both methods produced tissues rich in sulfated glycosaminoglycan and collagen, although the extracellular matrix produced on chloroform\u2010cast films appeared to contain more collagen type <jats:styled-content style=\"fixed-case\">II<\/jats:styled-content> and less collagen type <jats:styled-content style=\"fixed-case\">I<\/jats:styled-content> than acetone\u2010cast films. These self\u2010assembled constructs have the potential to be implanted into defect sites as a potential treatment for cartilage defect regeneration.<\/jats:p>","DOI":"10.1111\/aor.12565","type":"journal-article","created":{"date-parts":[[2015,10,30]],"date-time":"2015-10-30T13:42:00Z","timestamp":1446212520000},"page":"376-384","source":"Crossref","is-referenced-by-count":20,"title":["Self\u2010Assembled Infrapatellar Fat\u2010Pad Progenitor Cells on a Poly\u2010\u03b5\u2010Caprolactone Film For Cartilage Regeneration"],"prefix":"10.1111","volume":"40","author":[{"given":"Alisha","family":"Prabhakar","sequence":"first","affiliation":[{"name":"Trinity Centre for Bioengineering, Trinity Biomedical Sciences Institute Trinity College Dublin  Dublin Ireland"},{"name":"Department of Mechanical and Manufacturing Engineering, School of Engineering Trinity College Dublin  Dublin Ireland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Amy P.","family":"Lynch","sequence":"additional","affiliation":[{"name":"Trinity Centre for Bioengineering, Trinity Biomedical Sciences Institute Trinity College Dublin  Dublin Ireland"},{"name":"Department of Mechanical and Manufacturing Engineering, School of Engineering Trinity College Dublin  Dublin Ireland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mark","family":"Ahearne","sequence":"additional","affiliation":[{"name":"Trinity Centre for Bioengineering, Trinity Biomedical Sciences Institute Trinity College Dublin  Dublin Ireland"},{"name":"Department of Mechanical and Manufacturing Engineering, School of Engineering Trinity College Dublin  Dublin Ireland"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2015,10,30]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1093\/rheumatology\/kei018"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.2174\/157488812799219009"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0142-9612(00)00121-6"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0142-9612(99)00213-6"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2004.03.005"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actbio.2006.02.005"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/jor.21314"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1089\/ten.tec.2011.0011"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1089\/ten.tec.2013.0118"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1089\/ten.tea.2014.0035"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.progpolymsci.2007.05.017"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/pat.733"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.3109\/14653240903219122"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/bab.45"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.22203\/eCM.v023a09"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1089\/ten.tea.2013.0267"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1186\/2045-9769-3-13"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1088\/1748-6041\/8\/3\/035004"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2003.12.003"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1006\/jcis.1995.1167"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/0142-9418(82)90010-1"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1177\/0885328210376997"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2006.06.044"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200405004"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0015978"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmat2013"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmat3058"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2012.03.053"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.1039\/b910132m"},{"key":"e_1_2_6_31_1","doi-asserted-by":"publisher","DOI":"10.1529\/biophysj.108.132217"},{"key":"e_1_2_6_32_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.67.2.400"},{"key":"e_1_2_6_33_1","doi-asserted-by":"publisher","DOI":"10.1002\/aja.1001650102"},{"key":"e_1_2_6_34_1","doi-asserted-by":"publisher","DOI":"10.1006\/excr.1995.1069"},{"key":"e_1_2_6_35_1","doi-asserted-by":"publisher","DOI":"10.22203\/eCM.v008a06"},{"key":"e_1_2_6_36_1","doi-asserted-by":"publisher","DOI":"10.1134\/S106193480701011X"}],"container-title":["Artificial 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