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However, new strategies are needed to improve the treatment of skin wounds. In this study, we developed and characterized a new device consisting of poly(lactic\u2010co\u2010glycolic acid) (<jats:styled-content style=\"fixed-case\">PLGA<\/jats:styled-content>) and collagen associated with mesenchymal stem cells derived from human adipose tissue. To develop the bilaminar device, we initially obtained a membrane of <jats:styled-content style=\"fixed-case\">PLGA<\/jats:styled-content> by dissolving the copolymer in chloroform and then produced a collagen type <jats:styled-content style=\"fixed-case\">I<\/jats:styled-content> scaffold by freeze\u2010drying. The materials were characterized physically by gel permeation chromatography, scanning electron microscopy, and mass loss. Biological activity was assessed by cell proliferation assay. A preliminary study in vivo was performed with a pig model in which tissue regeneration was assessed macroscopically and histologically, the commercial device Integra being used as a control. The <jats:styled-content style=\"fixed-case\">PLGA<\/jats:styled-content>\/collagen bilaminar material was porous, hydrolytically degradable, and compatible with skin growth. The polymer complex allowed cell adhesion and proliferation, making it a potentially useful cell carrier. In addition, the transparency of the material allowed monitoring of the lesion when the dressings were changed. Xenogeneic mesenchymal cells cultured on the device (<jats:styled-content style=\"fixed-case\">PLGA<\/jats:styled-content>\/collagen\/<jats:styled-content style=\"fixed-case\">ASC<\/jats:styled-content>) showed a reduced granulomatous reaction to bovine collagen, down\u2010regulation of \u03b1\u2010<jats:styled-content style=\"fixed-case\">SMA<\/jats:styled-content>, enhancement in the number of neoformed blood vessels, and collagen organization as compared with normal skin; the device was superior to other materials tested (<jats:styled-content style=\"fixed-case\">PLGA<\/jats:styled-content>\/collagen and Integra) in its ability to stimulate the formation of new cutaneous tissue.<\/jats:p>","DOI":"10.1111\/aor.12671","type":"journal-article","created":{"date-parts":[[2016,1,11]],"date-time":"2016-01-11T06:33:08Z","timestamp":1452493988000},"page":"938-949","source":"Crossref","is-referenced-by-count":13,"title":["Bilaminar Device of Poly(Lactic\u2010co\u2010Glycolic Acid)\/Collagen Cultured With Adipose\u2010Derived Stem Cells for Dermal Regeneration"],"prefix":"10.1111","volume":"40","author":[{"given":"Juliana A.","family":"Domingues","sequence":"first","affiliation":[{"name":"Department of Cell Biology and Structural Biology Institute UNICAMP  Campinas Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Giselle","family":"Cherutti","sequence":"additional","affiliation":[{"name":"Department of Materials Engineering Faculty of Mechanical Engineering UNICAMP  Campinas Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Adriana C.","family":"Motta","sequence":"additional","affiliation":[{"name":"Department of Physiological Sciences Biomaterials Laboratory PUC\u2010 SP  Sorocaba Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Moema A.","family":"Hausen","sequence":"additional","affiliation":[{"name":"Department of Biology Structural Biology Laboratory UFSCAR  Sorocaba 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