{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,24]],"date-time":"2026-06-24T17:05:53Z","timestamp":1782320753071,"version":"3.54.5"},"reference-count":14,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2016,5,16]],"date-time":"2016-05-16T00:00:00Z","timestamp":1463356800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000738","name":"U.S. Department of Veterans Affairs","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000738","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["AR060408"],"award-info":[{"award-number":["AR060408"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Artificial Organs"],"published-print":{"date-parts":[[2016,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Diagnosis of cartilage damage in early stages of arthritis is vital to impede the progression of disease. In this regard, considerable progress has been made in near\u2010infrared fluorescence (NIRF) optical imaging technique. Arthritis can develop due to various mechanisms but one of the main contributors is the production of matrix metalloproteinases (MMPs), enzymes that can degrade components of the extracellular matrix. Especially, MMP\u20101 and MMP\u201013 have main roles in rheumatoid arthritis and osteoarthritis because they enhance collagen degradation in the process of arthritis. We present here a novel NIRF imaging strategy that can be used to determine the activity of MMPs and cartilage damage simultaneously by detection of exposed type II collagen in cartilage tissue. In this study, retro\u2010orbital injection of mixed fluorescent dyes, MMPSense 750 FAST (MMP750) dye and Alexa Fluor 680 conjugated monoclonal mouse antibody immune\u2010reactive to type II collagen, was administered in the arthritic mice. Both dyes were detected with different intensity according to degree of joint destruction in the animal. Thus, our dual fluorescence imaging method can be used to detect cartilage damage as well as MMP activity simultaneously in early stage arthritis.<\/jats:p>","DOI":"10.1111\/aor.12685","type":"journal-article","created":{"date-parts":[[2016,5,16]],"date-time":"2016-05-16T20:42:40Z","timestamp":1463431360000},"page":"1009-1013","source":"Crossref","is-referenced-by-count":10,"title":["In Vivo Dual Fluorescence Imaging to Detect Joint Destruction"],"prefix":"10.1111","volume":"40","author":[{"given":"Hongsik","family":"Cho","sequence":"first","affiliation":[{"name":"Department of Orthopaedic Surgery and Biomedical Engineering University of Tennessee Health Science Center Memphis TN USA"},{"name":"Veterans Affairs Medical Center Memphis TN USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fazal\u2010Ur\u2010Rehman","family":"Bhatti","sequence":"additional","affiliation":[{"name":"Department of Orthopaedic Surgery and Biomedical Engineering University of Tennessee Health Science Center Memphis TN USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sangmin","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Neurology University of Tennessee Health Science Center Memphis TN USA"},{"name":"Veterans Affairs Medical Center Memphis TN USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David D.","family":"Brand","sequence":"additional","affiliation":[{"name":"Department of Medicine University of Tennessee Health Science Center Memphis TN USA"},{"name":"Veterans Affairs Medical Center Memphis TN USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ae\u2010Kyung","family":"Yi","sequence":"additional","affiliation":[{"name":"Department of Microbiology Immunology and Biochemistry, University of Tennessee Health Science Center Memphis TN USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karen A.","family":"Hasty","sequence":"additional","affiliation":[{"name":"Department of Orthopaedic Surgery and Biomedical Engineering University of Tennessee Health Science Center Memphis TN USA"},{"name":"Veterans Affairs Medical Center Memphis TN USA"},{"name":"Campbell Clinic Memphis TN USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2016,5,16]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.20344\/amp.5477"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrrheum.2015.95"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1258\/jrsm.97.9.421"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.3899\/jrheum.141244"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/art.20379"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1186\/ar1483"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.2741\/1817"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.nano.2015.01.011"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.nano.2013.09.004"},{"key":"e_1_2_8_11_1","doi-asserted-by":"crossref","first-page":"2103","DOI":"10.4049\/jimmunol.148.7.2103","article-title":"Induction of arthritis with monoclonal antibodies to collagen","volume":"148","author":"Terato K","year":"1992","journal-title":"J Immunol"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.185.6.1113"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2007.173"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/art.22353"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.7326\/0003-4819-146-6-200703200-00005"}],"container-title":["Artificial Organs"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Faor.12685","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/aor.12685","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,26]],"date-time":"2023-09-26T06:25:35Z","timestamp":1695709535000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/aor.12685"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,5,16]]},"references-count":14,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2016,10]]}},"alternative-id":["10.1111\/aor.12685"],"URL":"https:\/\/doi.org\/10.1111\/aor.12685","archive":["Portico"],"relation":{},"ISSN":["0160-564X","1525-1594"],"issn-type":[{"value":"0160-564X","type":"print"},{"value":"1525-1594","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,5,16]]}}}