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The graft was reperfused with oxygenated buffer with or without hydrogen at 37\u00b0 for 90 min on an isolated perfusion apparatus, comprising the H<jats:sub>2<\/jats:sub>(+) and H<jats:sub>2<\/jats:sub>(\u2212) groups, respectively. In the control group (CT), grafts were reperfused immediately without preservation. Graft function, injury, and circulatory status were assessed throughout the perfusion. Tissue samples at the end of perfusion were collected to determine histopathology, oxidative stress, and apoptosis. In the H<jats:sub>2<\/jats:sub>(\u2212) group, IRI was indicated by a higher aspartate aminotransferase (AST), alanine aminotransferase (ALT) leakage, portal resistance, 8\u2010hydroxy\u20102\u2010deoxyguanosine\u2010positive cell rate, apoptotic index, and endothelial endothelin\u20101 expression, together with reduced bile production, oxygen consumption, and GSH\/GSSG ratio (vs. CT). In the H<jats:sub>2<\/jats:sub>(+) group, these harmful changes were significantly suppressed [vs. H<jats:sub>2<\/jats:sub>(\u2212)]. Hydrogen gas reduced hepatic reperfusion injury after prolonged cold preservation via the maintenance of portal flow, by protecting mitochondrial function during the early phase of reperfusion, and via the suppression of oxidative stress and inflammatory cascades thereafter.<\/jats:p>","DOI":"10.1111\/aor.12710","type":"journal-article","created":{"date-parts":[[2016,5,3]],"date-time":"2016-05-03T02:32:28Z","timestamp":1462242748000},"page":"1128-1136","source":"Crossref","is-referenced-by-count":27,"title":["Hydrogen Gas Ameliorates Hepatic Reperfusion Injury After Prolonged Cold Preservation in Isolated Perfused Rat Liver"],"prefix":"10.1111","volume":"40","author":[{"given":"Shingo","family":"Shimada","sequence":"first","affiliation":[{"name":"Department of Gastroenterological Surgery I"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kenji","family":"Wakayama","sequence":"additional","affiliation":[{"name":"Department of Gastroenterological Surgery 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