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Novel device designs are being developed to reduce the blood trauma, which will need to undergo in vitro and in vivo preclinical testing in large animal models such as cattle, sheep, and pig. To fully understand the impact of device design and enable translation of preclinical results, it is important to identify any potential species\u2010specific differences in the VAD\u2010associated common complications. Therefore, the purpose of this study was to evaluate the effects of shear stress on cells and proteins in bovine, ovine, and porcine blood compared to human. Blood from different species was subjected to various shear rates (0\u20138000\/s) using a rheometer. It was then analyzed for complete blood counts, hemolysis by the Harboe assay, platelet activation by flow cytometry, vWF structure by immunoblotting, and function by collagen binding activity ELISA (vWF\u00a0:\u00a0CBA). Overall, increasing shear rate caused increased total blood trauma in all tested species. This analysis revealed species\u2010specific differences in shear\u2010induced hemolysis, platelet activation, and vWF structure and function. Compared to human blood, porcine blood was the most resilient and showed less hemolysis, similar blood counts, but less platelet activation and less vWF damage in response to shear. Compared to human blood, sheared bovine blood showed less hemolysis, similar blood cell counts, greater platelet activation, and similar degradation of vWF structure, but less impact on its activity in response to shear. The shear\u2010induced effect on ovine blood depended on whether the blood was collected via gravity at the abattoir or by venepuncture from live sheep. Overall, ovine abattoir blood was the least resilient in response to shear and bovine blood was the most similar to human blood. These results lay the foundations for developing blood trauma evaluation standards to enable the extrapolation of in vitro and in vivo animal data to predict safety and biocompatibility of blood\u2010handling medical devices in humans. We advise using ovine venepuncture blood instead of ovine abattoir blood due to the greater overall damage in the latter. We propose using bovine blood for total blood damage in vitro device evaluation but multiple species could be used to create a full understanding of the complication risk profile of new devices. Further, this study highlights that choice of antibody clone for evaluating platelet activation in bovine blood can influence the interpretation of results from different studies.<\/jats:p>","DOI":"10.1111\/aor.12932","type":"journal-article","created":{"date-parts":[[2017,7,26]],"date-time":"2017-07-26T07:56:40Z","timestamp":1501055800000},"page":"934-947","source":"Crossref","is-referenced-by-count":71,"title":["Shear Stress\u2010Induced Total Blood Trauma in Multiple Species"],"prefix":"10.1111","volume":"41","author":[{"given":"Chris H. H.","family":"Chan","sequence":"first","affiliation":[{"name":"Institute of Life Science Swansea University Medical School Swansea Wales UK"},{"name":"Institute of Life Science Calon Cardio\u2010Technology Ltd. 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