{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T00:44:22Z","timestamp":1787013862375,"version":"build-2736575974"},"reference-count":35,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2017,7,19]],"date-time":"2017-07-19T00:00:00Z","timestamp":1500422400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100004587","name":"Instituto de Salud Carlos III","doi-asserted-by":"publisher","award":["FIS PI13\/01768"],"award-info":[{"award-number":["FIS PI13\/01768"]}],"id":[{"id":"10.13039\/501100004587","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004587","name":"Instituto de Salud Carlos III","doi-asserted-by":"publisher","award":["FIS PI15\/00255"],"award-info":[{"award-number":["FIS PI15\/00255"]}],"id":[{"id":"10.13039\/501100004587","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Artificial Organs"],"published-print":{"date-parts":[[2017,11]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Several cases of patients with anaphylactic or systemic hypersensitivity reactions to polysulfone (PS) hemodialysis (HD) membranes and tolerance to cellulose triacetate (CTA) membranes have recently been reported. To investigate the mechanisms involved in PS hypersensitivity, basophil, T cell, and complement activation were analyzed in acute\u2010phase samples from two patients with systemic reactions to PS\u2010based membranes. Basophil and T cell activation, as well as higher serum tryptase levels were detected in acute\u2010phase samples compared with basal levels. Complement levels (C3 and C4) were decreased in acute\u2010phase samples from PS\u2010allergic patients to a higher extent than in samples from control donors taken at the same time points, indicating complement activation during the acute reactions. An experimental external circuit was established on pediatric membranes after rinsing with low or high priming volumes of saline solution, to analyze basophils, T cells, and complement activation in blood samples from 10 PS\u2010allergic and 8 nonallergic HD patients upon contact with PS\u2010based or CTA membranes. Predialysis and postdialysis samples were collected. Basophils from PS\u2010allergic patients exhibited increased degranulation, and T cells showed significantly increased activation after contact with PS\u2010based membranes primed with low volumes of saline. No activation was detected in leukocytes from nonallergic patients under the same experimental conditions. Membrane priming with high volumes of saline abrogated activation of basophils and T cells. However, basophils from allergic donors showed significantly higher responses to Fc\u03b5c stimulation after contact with PS membranes. Basophil degranulation and elevated serum tryptase levels in allergic patients during acute reactions support the systemic activation of mast cells and basophils during hypersensitivity reactions to PS\u2010based membranes. A leachable component of the membranes might be responsible for cell activation in some patients.<\/jats:p>","DOI":"10.1111\/aor.12954","type":"journal-article","created":{"date-parts":[[2017,7,19]],"date-time":"2017-07-19T07:58:39Z","timestamp":1500451119000},"source":"Crossref","is-referenced-by-count":19,"title":["Mechanisms Involved in Hypersensitivity Reactions to Polysulfone Hemodialysis Membranes"],"prefix":"10.1111","volume":"41","author":[{"given":"Aranzazu","family":"Rodr\u00edguez\u2010Sanz","sequence":"first","affiliation":[{"name":"Hospital La Paz Health Research Institute\u2010IdiPAZ\u2014Research Madrid Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rafael","family":"S\u00e1nchez\u2010Villanueva","sequence":"additional","affiliation":[{"name":"Hospital La Paz Health Research Institute\u2010IdiPAZ\u2014Research Madrid 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