{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,21]],"date-time":"2026-07-21T18:46:26Z","timestamp":1784659586826,"version":"3.55.0"},"reference-count":75,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2019,2,27]],"date-time":"2019-02-27T00:00:00Z","timestamp":1551225600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100008323","name":"Zanjan University of Medical Sciences","doi-asserted-by":"publisher","award":["A-10-892-15."],"award-info":[{"award-number":["A-10-892-15."]}],"id":[{"id":"10.13039\/501100008323","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Artificial Organs"],"published-print":{"date-parts":[[2019,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>An electrical stimulus is a new approach to neural differentiation of stem cells. In this work, the neural differentiation of conjunctiva mesenchymal stem cells (CJMSCs) on a new 3D conductive fibrous scaffold of silk fibroin (SF) and reduced graphene oxide (rGo) were examined. rGo (3.5% w\/w) was dispersed in SF\u2010acid formic solution (10% w\/v) and conductive nanofibrous scaffold was fabricated using the electrospinning method. SEM and TEM microscopies were used for fibrous scaffold characterization. CJMSCs were cultured on the scaffold and 2 electrical impulse models (Current 1:115\u00a0V\/m, 100\u2010Hz frequency and current 2:115\u00a0v\/m voltages, 0.1\u2010Hz frequency) were applied for 7\u00a0days. Also, the effect of the fibrous scaffold and electrical impulses on cell viability and neural gene expression were examined using MTT assay and qPCR analysis. Fibrous scaffold with the 220\u00a0\u00b1\u00a020\u00a0nm diameter and good dispersion of graphene nanosheets at the surface of nanofibers were fabricated. The MTT result showed the viability of cells on the scaffold, with current 2 lower than current 1. qPCR analysis confirmed that the expression of \u03b2\u2010tubulin (2.4\u2010fold <jats:italic>P<\/jats:italic>\u00a0\u2264\u00a00.026), MAP\u20102 (1.48\u2010fold; <jats:italic>P<\/jats:italic>\u00a0\u2264\u00a00.03), and nestin (1.5\u2010fold; <jats:italic>P<\/jats:italic>\u00a0\u2264\u00a00.03) genes were higher in CJMSCs on conductive scaffold with 100\u2010Hz frequency compared to 0.1\u2010Hz frequency. Collectively, we proposed that SF\u2010rGo fibrous scaffolds, as a new conductive fibrous scaffold with electrical stimulation are good strategies for neural differentiation of stem cells and the type of electrical pulses has an influence on neural differentiation and proliferation of CJMSCs.<\/jats:p>","DOI":"10.1111\/aor.13425","type":"journal-article","created":{"date-parts":[[2019,1,24]],"date-time":"2019-01-24T19:12:22Z","timestamp":1548357142000},"page":"780-790","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":49,"title":["Conductive electrospun scaffolds with electrical stimulation for neural differentiation of conjunctiva mesenchymal stem cells"],"prefix":"10.1111","volume":"43","author":[{"given":"Ali","family":"Rahmani","sequence":"first","affiliation":[{"name":"Department of Medical Nanotechnology Zanjan University of Medical Sciences Zanjan Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1523-3152","authenticated-orcid":false,"given":"Samad","family":"Nadri","sequence":"additional","affiliation":[{"name":"Department of Medical Nanotechnology Zanjan University of Medical Sciences Zanjan Iran"},{"name":"Zanjan Metabolic Diseases Research Center Zanjan University of Medical Sciences Zanjan Iran"},{"name":"Zanjan Pharmaceutical Nanotechnology Research Center Zanjan University of Medical Sciences Zanjan Iran"},{"name":"Cancer Gene Therapy Research Center Zanjan University of Medical Sciences Zanjan Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Habib Sayed","family":"Kazemi","sequence":"additional","affiliation":[{"name":"Department of Chemistry Institute for Advanced Studies in Basic Sciences (IASBS) Zanjan Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yousef","family":"Mortazavi","sequence":"additional","affiliation":[{"name":"Cancer Gene Therapy Research Center Zanjan University of Medical Sciences Zanjan Iran"},{"name":"Department of Medical Biotechnology Zanjan University of Medical Sciences Zanjan Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mahdi","family":"Sojoodi","sequence":"additional","affiliation":[{"name":"Department of Electrical and Computer Engineering Tarbiat Modares University Tehran 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