{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,9]],"date-time":"2026-08-09T11:41:16Z","timestamp":1786275676763,"version":"build-2736575974"},"reference-count":26,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2019,7,18]],"date-time":"2019-07-18T00:00:00Z","timestamp":1563408000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2019,7,18]],"date-time":"2019-07-18T00:00:00Z","timestamp":1563408000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100003443","name":"Ministry of Education and Science of the Russian Federation","doi-asserted-by":"publisher","award":["1201253306"],"award-info":[{"award-number":["1201253306"]}],"id":[{"id":"10.13039\/501100003443","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003443","name":"Ministry of Education and Science of the Russian Federation","doi-asserted-by":"publisher","award":["6.5882.2017"],"award-info":[{"award-number":["6.5882.2017"]}],"id":[{"id":"10.13039\/501100003443","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002261","name":"Russian Foundation for Basic Research","doi-asserted-by":"publisher","award":["15-29-01252"],"award-info":[{"award-number":["15-29-01252"]}],"id":[{"id":"10.13039\/501100002261","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002261","name":"Russian Foundation for Basic Research","doi-asserted-by":"publisher","award":["17-03-01361a"],"award-info":[{"award-number":["17-03-01361a"]}],"id":[{"id":"10.13039\/501100002261","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Artificial Organs"],"published-print":{"date-parts":[[2019,11]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Development of artificial tissues or organs is one of the actual tasks in regenerative medicine that requires observation and evaluation of intact volume microstructure of tissue engineering products at all stages of their formation, from native donor tissues and decellularized scaffolds to recipient cell migration in the matrix. Unfortunately in practice, methods of vital noninvasive imaging of volume microstructure in matrixes are absent. In this work, we propose a new approach based on high\u2010frequency acoustic microscopy for noninvasive evaluation and visualization of volume microstructure in tissue engineering products. The results present the ultrasound characterization of native rat diaphragms and lungs and their decellularized scaffolds. Verification of the method for visualization of tissue formation in the matrix volume was described in the model samples of diaphragm scaffolds with stepwise collagenization. Results demonstrate acoustic microscopic sensitivity to cell content concentration, variation in\u00a0local density, and orientation of protein fibers in the volume, micron air inclusions, and other inhomogeneities of matrixes.<\/jats:p>","DOI":"10.1111\/aor.13516","type":"journal-article","created":{"date-parts":[[2019,6,14]],"date-time":"2019-06-14T02:03:53Z","timestamp":1560477833000},"page":"1104-1110","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["Noninvasive ultrasound imaging for assessment of intact microstructure of extracellular matrix in tissue engineering"],"prefix":"10.1111","volume":"43","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7820-7947","authenticated-orcid":false,"given":"Egor","family":"Morokov","sequence":"first","affiliation":[{"name":"Laboratory of Acoustic Microscopy N.M. Emanuel Institute of Biochemical Physics Russian Academy of Sciences Moscow Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elena","family":"Khramtsova","sequence":"additional","affiliation":[{"name":"Laboratory of Acoustic Microscopy N.M. Emanuel Institute of Biochemical Physics Russian Academy of Sciences Moscow Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elena","family":"Kuevda","sequence":"additional","affiliation":[{"name":"Laboratory of Fundamental Research in the Field of Regenerative Medicine Kuban State Medical University Krasnodar Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elena","family":"Gubareva","sequence":"additional","affiliation":[{"name":"Laboratory of Fundamental Research in the Field of Regenerative Medicine Kuban State Medical University Krasnodar Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Timothei","family":"Grigoriev","sequence":"additional","affiliation":[{"name":"National Research Centre \u201cKurchatov Institute\u201d (NRC \u201cKurchatov Institute\u201d) Moscow Russia"},{"name":"Institute of Organoelement Compounds Russian Academy of Sciences Moscow Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ksenia","family":"Lukanina","sequence":"additional","affiliation":[{"name":"National Research Centre \u201cKurchatov Institute\u201d (NRC \u201cKurchatov Institute\u201d) Moscow Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vadim","family":"Levin","sequence":"additional","affiliation":[{"name":"Laboratory of Acoustic Microscopy N.M. Emanuel Institute of Biochemical Physics Russian Academy of Sciences Moscow Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2019,7,18]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-bioeng-071910-124743"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1155\/2011\/290602"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.3109\/10520295.2014.957724"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.2967\/jnumed.115.170548"},{"key":"e_1_2_7_6_1","doi-asserted-by":"crossref","first-page":"444","DOI":"10.1007\/978-94-007-6952-6","volume-title":"Quantitative ultrasound in soft tissues","author":"Mamou J","year":"2013"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ultras.2015.03.010"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1114\/1.1424920"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ultrasmedbio.2006.07.037"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/0301-5629(91)90096-F"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0122327"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.7863\/jum.1997.16.2.117"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1177\/0161734614524179"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2013.08.033"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actbio.2015.12.029"},{"key":"e_1_2_7_16_1","first-page":"3","article-title":"Ultrasonic characterization of extra\u2010cellular matrix in decellularized murine kidney and liver","volume":"2015","author":"Wirtzfeld LA","year":"2015","journal-title":"IEEE Int Ultraso Symp Proc"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10439-014-0994-8"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2010.07.089"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2013.01.036"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbiosc.2011.10.011"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1189345"},{"key":"e_1_2_7_22_1","first-page":"38","article-title":"Experience of perfusion recellularization of biological lung scaffold in rats","volume":"18","author":"Kuevda EV","year":"2016","journal-title":"Vestnik Transplantologii i Iskusstvennykh Organov"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.carbpol.2018.02.061"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1002\/pen.24617"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1002\/pssb.201600077"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.3103\/S1062873815100184"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1109\/ULTSYM.1994.401909"}],"container-title":["Artificial Organs"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/aor.13516","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/aor.13516","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/aor.13516","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,9]],"date-time":"2026-08-09T10:53:14Z","timestamp":1786272794000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/aor.13516"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,7,18]]},"references-count":26,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2019,11]]}},"alternative-id":["10.1111\/aor.13516"],"URL":"https:\/\/doi.org\/10.1111\/aor.13516","archive":["Portico"],"relation":{},"ISSN":["0160-564X","1525-1594"],"issn-type":[{"value":"0160-564X","type":"print"},{"value":"1525-1594","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,7,18]]},"assertion":[{"value":"2019-03-04","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-06-06","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-07-18","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}