{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,14]],"date-time":"2025-05-14T00:41:50Z","timestamp":1747183310256,"version":"3.40.5"},"reference-count":46,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2019,12,15]],"date-time":"2019-12-15T00:00:00Z","timestamp":1576368000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","award":["140726","2013\u20106","205902","2014\u20106"],"award-info":[{"award-number":["140726","2013\u20106","205902","2014\u20106"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Artificial Organs"],"published-print":{"date-parts":[[2020,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Mechanical evaluation of tracheal grafts is of great relevance for transplant research. Although there are some publications demonstrating different techniques of tracheal mechanical evaluation, there is still no definitive or preferred protocol available. Here, we present a simple image processing acquisition system that can be used for in vivo experiments. Six male Wistar rats were submitted to orotracheal intubation and a longitudinal incision was made to expose the trachea. Images of tracheae were acquired from a video camera in different scenarios of bronchoconstriction using methacholine (MCh) (Basal, PBS, MCh 30\u00a0\u03bcg\/kg, MCh 300\u00a0\u03bcg\/kg, and postmetabolized) during imposed\u2010inspiration and imposed\u2010expiration. The area variation ratio (the ratio between areas during expiration vs. inspiration) was 1.1\u00d7 for the Basal group, while the ratio for MCh 300\u00a0\u00b5g\/kg was 6.5\u00d7. The area variation of imaged tracheae was statistically significant at the dose of MCh 300\u00a0\u00b5g\/kg for imposed\u2010inspiration versus imposed\u2010expiration (<jats:italic>P<\/jats:italic>\u00a0=\u00a0.002). Likewise, elastance data of respiratory mechanics indicated a\u00a0statistically significant difference at the dose of MCh 300\u00a0\u00b5g\/kg for imposed\u2010inspiration versus imposed\u2010expiration (<jats:italic>P<\/jats:italic>\u00a0=\u00a0.026). Our image processing analysis protocol presented corresponding behavior when compared to mechanical parameters of the respiratory system. In addition, our image acquisition system was able to highlight the differences between imposed\u2010inspiration and imposed\u2010expiration. Image analysis of the tracheal area variation seems to be in agreement with the elastance of the respiratory system. Taken together, these observations may help future studies of tracheal transplantation for in situ assessment of graft patency.<\/jats:p>","DOI":"10.1111\/aor.13604","type":"journal-article","created":{"date-parts":[[2019,11,12]],"date-time":"2019-11-12T19:35:31Z","timestamp":1573587331000},"page":"504-512","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["An in vivo image acquisition system for the evaluation of tracheal mechanics in rats"],"prefix":"10.1111","volume":"44","author":[{"given":"Marcelo Henrique","family":"Valenga","sequence":"first","affiliation":[{"name":"Biomedical Engineering Laboratory (LEB) Escola Polit\u00e9cnica, University of S\u00e3o Paulo S\u00e3o Paulo \u2013 SP Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Renato de Lima","family":"Vitorasso","sequence":"additional","affiliation":[{"name":"Biomedical Engineering Laboratory (LEB) Escola Polit\u00e9cnica, University 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Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Henrique Takachi","family":"Moriya","sequence":"additional","affiliation":[{"name":"Biomedical Engineering Laboratory (LEB) Escola Polit\u00e9cnica, University of S\u00e3o Paulo S\u00e3o Paulo \u2013 SP Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3387-5121","authenticated-orcid":false,"given":"Fabio Gava","family":"Aoki","sequence":"additional","affiliation":[{"name":"Biomedical Engineering Laboratory (LEB) Escola Polit\u00e9cnica, University of S\u00e3o Paulo S\u00e3o Paulo \u2013 SP Brazil"},{"name":"Institute of Science and Technology (ICT) Federal University of S\u00e3o Paulo S\u00e3o Jos\u00e9 dos Campos \u2013 SP 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