{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T02:37:45Z","timestamp":1792636665664,"version":"4.5.0"},"reference-count":14,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2020,4,30]],"date-time":"2020-04-30T00:00:00Z","timestamp":1588204800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100014219","name":"National Science Fund for Distinguished Young Scholars","doi-asserted-by":"publisher","award":["81525002"],"award-info":[{"award-number":["81525002"]}],"id":[{"id":"10.13039\/501100014219","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Artificial Organs"],"published-print":{"date-parts":[[2020,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>In heart transplantation, time restriction is an unavoidable thorny problem during cardiac transport. Cold storage is an important organ preservation method in donor heart transport. Cold\u2010inducible RNA binding protein (CIRBP) has been proven to play a protective role under cold stress. In this study, we investigated the role of CIRBP in hypothermic cardioprotection during heart preservation in UW solution and explored a new approach to extend the heart preservation time. <jats:italic>Cirbp<\/jats:italic>\u2010knockout (<jats:italic>Cirbp<\/jats:italic><jats:sup>\u2212\/\u2212<\/jats:sup>), <jats:italic>Cirbp<\/jats:italic>\u2010transgenic (<jats:italic>Cirbp<\/jats:italic>\u2010Tg), and wild\u2010type rats were, respectively, randomized into two groups based on various heart preservation times (6 or 12\u2010hour group) (n\u00a0=\u00a08 per group). After preservation in UW solution, all hearts were mounted on a\u00a0Langendorff apparatus and\u00a0underwent measurement of cardiac parameters, histological analysis, and molecular study. Within the 6\u2010hour preservation group, no significant difference was found in cardiac functions and histological changes between different rat species. However, after 12 hours of preservation, <jats:italic>Cirbp<\/jats:italic><jats:sup>\u2212\/\u2212<\/jats:sup> rat hearts showed more apoptosis and worse cardiac function, but less apoptosis and better cardiac function were observed in <jats:italic>Cirbp<\/jats:italic>\u2010Tg rat hearts. Furthermore, we found CIRBP\u2010mediated cardiac ubiquinone (CoQ<jats:sub>10<\/jats:sub>) biosynthesis plays an important role in extending heart preservation, and ubiquinone biosynthesis protein COQ9 was an essential down\u2010stream regulator during this process. Finally, we found that zr17\u20102, a CIRBP agonist, could enhance the expression of CIRBP, which further enhances the synthesis of CoQ<jats:sub>10<\/jats:sub> and promotes scavenging of reactive oxygen species and ATP production to extend heart preservation. This study demonstrated that CIRBP\u2010enhanced CoQ<jats:sub>10<\/jats:sub> biosynthesis during hypothermic heart preservation and zr17\u20102\u2010supplemented UW solution could be a promising approach to ameliorate heart damage and extend heart preservation during cardiac transport.<\/jats:p>","DOI":"10.1111\/aor.13695","type":"journal-article","created":{"date-parts":[[2020,4,12]],"date-time":"2020-04-12T11:04:26Z","timestamp":1586689466000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Cold\u2010inducible RNA binding protein agonist enhances the cardioprotective effect of UW solution during extended heart preservation"],"prefix":"10.1111","volume":"44","author":[{"given":"Hai\u2010Zhou","family":"Pan","sequence":"first","affiliation":[{"name":"Children\u2019s Heart Center the Second Affiliated Hospital and Yuying Children\u2019s Hospital Institute of Cardiovascular Development and Translational Medicine Wenzhou Medical University Wenzhou China"},{"name":"State Key Laboratory of Cardiovascular Disease Fuwai Hospital National Center for Cardiovascular Diseases Chinese Academy of Medical Sciences and Peking Union Medical College Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li\u2010Jing","family":"Zhang","sequence":"additional","affiliation":[{"name":"Children\u2019s Heart Center the Second Affiliated Hospital and Yuying Children\u2019s Hospital Institute of Cardiovascular Development and Translational Medicine Wenzhou Medical University Wenzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi\u2010Wei","family":"Liu","sequence":"additional","affiliation":[{"name":"Heart Center and Shanghai Institution of Pediatric Congenital Heart Diseases Shanghai Children\u2019s Medical Center and National Children\u2019s Medical Center Shanghai 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China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0206-9066","authenticated-orcid":false,"given":"Hao","family":"Zhang","sequence":"additional","affiliation":[{"name":"Children\u2019s Heart Center the Second Affiliated Hospital and Yuying Children\u2019s Hospital Institute of Cardiovascular Development and Translational Medicine Wenzhou Medical University Wenzhou China"},{"name":"State Key Laboratory of Cardiovascular Disease Fuwai Hospital National Center for Cardiovascular Diseases Chinese Academy of Medical Sciences and Peking Union Medical College Beijing China"},{"name":"Heart Center and Shanghai Institution of Pediatric Congenital Heart Diseases Shanghai Children\u2019s Medical Center and National Children\u2019s Medical Center Shanghai 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