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However, the function of these grafts after transplantation is not clear. The purpose of the study was to evaluate the early function of intestinal grafts after transplantation from expected cardiac death donors supported with normothermic extracorporeal support using a porcine allogeneic orthotopic segmental small bowel transplantation model. Eighteen domestic crossbred donor pigs were assigned to living donation (LD), donation after cardiac death (DCD), and ECMO groups. In the LD group, small bowels were harvested and preserved immediately in cold storage. In the other two groups, the donor pigs received conventional rapid recovery treatment or 1\u2010hour normothermic extracorporeal support after 10\u2010minutes expected cardiac arrest. Subsequently, the small bowels were removed and preserved in cold storage. After 5\u20106\u00a0hours of preservation, small bowel grafts were transplanted into the recipient pigs that underwent enterectomy. The pathology and electron microscopy results, cell apoptosis rate, tight junction protein expression level in the intestinal mucosa, and plasma endotoxin level were evaluated after transplantation. All grafts functioned on the basis of the maltose absorption test results at day 7 after transplantation. There were no significant differences in the morphological changes in the intestinal mucosa among the three groups at day 7 after transplantation. The cell apoptosis rate and plasma endotoxin level in the ECMO group did not differ significantly than those in the LD group, but were evidently lower than those in the DCD group (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a0.001). The intestinal absorptive function improved significantly in the ECMO group in contrast with that in the DCD group (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a0.001). Short\u2010term ECMO intervention can alleviate ischemia\u2013reperfusion injuries in intestinal grafts and improve intestinal absorptive function in the early stage after transplantation. Reducing caspase\u20103 protein expression and cell apoptosis in the intestinal mucosa may be one of the protective mechanisms of ECMO intervention.<\/jats:p>","DOI":"10.1111\/aor.13697","type":"journal-article","created":{"date-parts":[[2020,4,12]],"date-time":"2020-04-12T20:32:40Z","timestamp":1586723560000},"page":"1098-1106","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Normothermic extracorporeal membrane oxygenation support: Improving the function of intestinal grafts obtained from cardiac death donors"],"prefix":"10.1111","volume":"44","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4453-1749","authenticated-orcid":false,"given":"Mingxiao","family":"Guo","sequence":"first","affiliation":[{"name":"Department of General Surgery Linyi People's Hospital Linyi China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chunlei","family":"Lu","sequence":"additional","affiliation":[{"name":"Department of General Surgery Linyi People's Hospital Linyi China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Linlin","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Surgery Linyi Municipal Mental Health Center Linyi China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Danhua","family":"Yao","sequence":"additional","affiliation":[{"name":"Department of General Surgery Shanghai Ninth People's Hospital Shanghai JiaoTong University School of Medicine Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yousheng","family":"Li","sequence":"additional","affiliation":[{"name":"Department of General Surgery Shanghai Ninth People's Hospital Shanghai JiaoTong University School of Medicine Shanghai 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