{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,9]],"date-time":"2026-08-09T09:41:35Z","timestamp":1786268495603,"version":"build-2736575974"},"reference-count":49,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2020,8,15]],"date-time":"2020-08-15T00:00:00Z","timestamp":1597449600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2020,8,15]],"date-time":"2020-08-15T00:00:00Z","timestamp":1597449600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100000925","name":"National Health and Medical Research Council","doi-asserted-by":"publisher","award":["Centre for Research Excellence \/ APP1079421"],"award-info":[{"award-number":["Centre for Research Excellence \/ APP1079421"]}],"id":[{"id":"10.13039\/501100000925","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Artificial Organs"],"published-print":{"date-parts":[[2020,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Nonsurgical bleeding is the most frequent complication of left ventricular assist device (LVAD) support. Supraphysiologic shear rates generated in LVAD causes impaired platelet aggregation, which increases the risk of bleeding. The effect of shear rate on the formation size of platelet aggregates has never been reported experimentally, although platelet aggregation size can be considered to be directly relevant to bleeding complications. Therefore, this study investigated the impact of shear rate and exposure time on the formation size of platelet aggregates, which is vital in predicting bleeding in patients with an LVAD. Human platelet\u2010poor plasma (containing von Willebrand factor, vWF) and fluorochrome\u2010labeled platelets were subjected to a range of shear rates (0\u201010\u00a0000\u00a0s\n                    <jats:sup>\u22121<\/jats:sup>\n                    ) for 0, 5, 10, and 15\u00a0minutes using a custom\u2010built blood\u2010shearing device. Formed sizes of platelet aggregates under a range of shear\u2010controlled environment were visualized and measured using microscopy. The loss of high molecular weight (HMW) vWF multimers was quantified using gel electrophoresis and immunoblotting. An inhibition study was also performed to investigate the reduction in platelet aggregation size and HMW vWF multimers caused by either mechanical shear or enzymatic (a disintegrin and metalloproteinase with a thrombospondin type 1 motif, member 13\u2014ADAMTS13, the von Willebrand factor protease) mechanism under low and high shear conditions (360 and 10\u00a0000\u00a0s\n                    <jats:sup>\u22121<\/jats:sup>\n                    ). We found that the average size of platelet aggregates formed under physiological shear rates of 360\u20103000\u00a0s\n                    <jats:sup>\u22121<\/jats:sup>\n                    (200\u2010300\u00a0\u03bcm\n                    <jats:sup>2<\/jats:sup>\n                    ) was significantly larger compared to those sheared at &gt;6000\u00a0s\n                    <jats:sup>\u22121<\/jats:sup>\n                    (50\u2010100\u00a0\u03bcm\n                    <jats:sup>2<\/jats:sup>\n                    ). Furthermore, HMW vWF multimers were reduced with increased shear rates. The inhibition study revealed that the reduction in platelet aggregation size and HWM vWF multimers were mainly associated with ADAMTS13. In conclusion, the threshold of shear rate must not exceed &gt;6000\u00a0s\n                    <jats:sup>\u22121<\/jats:sup>\n                    in order to maintain the optimal size of platelet aggregates to \u201cplug off\u201d the injury site and stop bleeding.\n                  <\/jats:p>","DOI":"10.1111\/aor.13783","type":"journal-article","created":{"date-parts":[[2020,7,31]],"date-time":"2020-07-31T14:04:52Z","timestamp":1596204292000},"page":"1286-1295","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":26,"title":["Shear\u2010dependent platelet aggregation size"],"prefix":"10.1111","volume":"44","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7983-5614","authenticated-orcid":false,"given":"Chris Hoi Houng","family":"Chan","sequence":"first","affiliation":[{"name":"School of Engineering and Built Environment Griffith University Gold Coast QLD Australia"},{"name":"Critical Care Research Group The Prince Charles Hospital Brisbane QLD Australia"},{"name":"Faculty of Medicine University of Queensland Brisbane QLD Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masataka","family":"Inoue","sequence":"additional","affiliation":[{"name":"School of Engineering and Built Environment Griffith University Gold Coast QLD Australia"},{"name":"Critical Care Research Group The Prince Charles Hospital Brisbane QLD Australia"},{"name":"Department of Life Sciences Systems Engineering and Science Graduate School of Engineering and Science Shibaura Institute of Technology Saitama Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8818-5286","authenticated-orcid":false,"given":"Katrina K.","family":"Ki","sequence":"additional","affiliation":[{"name":"Critical Care Research Group The Prince Charles Hospital Brisbane QLD Australia"},{"name":"Faculty of Medicine University of Queensland Brisbane QLD Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tomotaka","family":"Murashige","sequence":"additional","affiliation":[{"name":"School of Engineering and Built Environment Griffith University Gold Coast QLD Australia"},{"name":"School of Engineering Tokyo Institute of Technology Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John F.","family":"Fraser","sequence":"additional","affiliation":[{"name":"Critical Care Research Group The Prince Charles Hospital Brisbane QLD Australia"},{"name":"Faculty of Medicine University of Queensland Brisbane QLD Australia"},{"name":"Menzies Health Institute Queensland Griffith University Gold Coast QLD Australia"},{"name":"School of Medicine Griffith University Gold Coast QLD Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael J.","family":"Simmonds","sequence":"additional","affiliation":[{"name":"Menzies Health Institute Queensland Griffith University Gold Coast QLD Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Geoff D.","family":"Tansley","sequence":"additional","affiliation":[{"name":"School of Engineering and Built Environment Griffith University Gold Coast QLD Australia"},{"name":"Critical Care Research Group The Prince Charles Hospital Brisbane QLD Australia"},{"name":"Menzies Health Institute Queensland Griffith University Gold Coast QLD Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nobuo","family":"Watanabe","sequence":"additional","affiliation":[{"name":"Department of Life Sciences Systems Engineering and Science Graduate School of Engineering and Science Shibaura Institute of Technology Saitama 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