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Generation of small vessels as vascular grafts or as envisioned central vessel for vascularized constructs is still a challenge. Here, the decellularization of porcine vessels by a non\u2010detergent based protocol was developed and investigated. Perfusion\u2010decellularization with sodium hydroxide solution resulted in removal of cellular material throughout the whole length of the vessel while preserving structural and mechanical integrity. A re\u2010endothelialization of the retrieved matrix with human umbilical vein endothelial cells and cardiac endothelial cells was achieved through rotation\u2010based seeding employing a custom\u2010made bioreactor. A confluent monolayer was detected on the entire luminal surface. Thus, a non\u2010detergent\u2010based decellularization method allowing the re\u2010endothelialization of the luminal surface was developed in this study, thereby paving the way for future implementation of the resulting construct as vascular graft or as central vessel for tissue engineered constructs in need of a perfusion system with readily available anastomosis sites.<\/jats:p>","DOI":"10.1111\/aor.13836","type":"journal-article","created":{"date-parts":[[2020,10,1]],"date-time":"2020-10-01T16:33:18Z","timestamp":1601569998000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Re\u2010endothelialization of non\u2010detergent decellularized porcine vessels"],"prefix":"10.1111","volume":"45","author":[{"given":"Katerina","family":"Eyre","sequence":"first","affiliation":[{"name":"Leibniz Research Laboratories for Biotechnology and Artificial Organs (LEBAO) Department of Cardiothoracic, Transplantation and Vascular 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