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To date, there are no specific antiviral drugs proven to be effective in treating SARS\u2010CoV\u20102, requiring joint efforts from different research fronts to discover the best route of treatment. The first decisions in drug discovery are based on 2D cell culture using high\u2010throughput screening. In this context, spheroids and organoids emerge as a reliable alternative. Both are scaffold\u2010free 3D engineered constructs that recapitulate key cellular and molecular events of tissue physiology. Different studies have already shown their advantages as a model for different infectious diseases, including SARS\u2010CoV\u20102 and for drug screening. The use of these 3D engineered tissues as an in vitro model can fill the gap between 2D cell culture and in vivo preclinical assays (animal models) as they could recapitulate the entire viral life cycle. The main objective of this review is to understand spheroid and organoid biology, highlighting their advantages and disadvantages, and how these scaffold\u2010free engineered tissues can contribute to a better comprehension of viral infection by SARS\u2010CoV\u20102 and to the development of in vitro high\u2010throughput models for drug screening.<\/jats:p>","DOI":"10.1111\/aor.13880","type":"journal-article","created":{"date-parts":[[2020,12,2]],"date-time":"2020-12-02T22:22:50Z","timestamp":1606947770000},"page":"548-558","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":42,"title":["Spheroids and organoids as humanized 3D scaffold\u2010free engineered tissues for SARS\u2010CoV\u20102 viral infection and drug screening"],"prefix":"10.1111","volume":"45","author":[{"given":"Gabriela S.","family":"Kronemberger","sequence":"first","affiliation":[{"name":"Nucleus of Multidisciplinary Research in Biology (Numpex\u2010Bio) Federal 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