{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,14]],"date-time":"2025-05-14T00:40:04Z","timestamp":1747183204376,"version":"3.40.5"},"reference-count":35,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2021,7,22]],"date-time":"2021-07-22T00:00:00Z","timestamp":1626912000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81970548"],"award-info":[{"award-number":["81970548"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Artificial Organs"],"published-print":{"date-parts":[[2022,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Ischemia\u2010reperfusion injury (IRI) is the major cause of delayed graft function (DGF) during the posttransplantation period. Estradiol (E2) prevents IRI\u2010induced kidney dysfunction and tissue injury. However, many side effects limit E2\u2019s in vivo application. Recent evidence uncovers E2\u2019s expanded use in the field of transplantation. We aimed to study if and how E2 exerts protective activity during the period of kidney organ preservation. The autologous kidney transplant model in rats was first established. Rats were divided into 5 groups: normal group (N), sham group (sham), static cold storage (SCS) 4\u00a0hours group (control), SCS 4\u00a0hours\u00a0+\u00a0ethanol (1\u00a0\u00b5L\/mL) group (solvent), and SCS 4\u00a0hours\u00a0+\u00a0ethanol (1\u00a0\u00b5L\/mL)\u00a0+\u00a0E2 (1000\u00a0ng\/mL) group (E2). ER\u03b1 expression under hypothermia was measured by western blotting. Moreover, biochemical analyses of plasma levels of creatinine, BUN, estradiol, and testosterone were examined. Among all groups, kidney tissues were collected and processed for further western blot analysis about ER\u03b1, eNOS, Bcl\u20102, and Bax expression, histological analyses such as H&amp;E staining to evaluate pathological severity. In\u00a0addition, a TUNEL assay is performed to evaluate apoptosis. E2 copreservation upregulated ER\u03b1 expression under hypothermia. Moreover, E2 copreservation reduced levels of creatinine and BUN in plasma but without affecting estradiol and testosterone. Further, E2 copreservation increased expression of eNOS and antiapoptotic Bcl\u20102 and decreases expression of proapoptotic Bax. E2 copreservation significantly inhibited IRI\u2010induced apoptosis and evidently improved pathological severity in the kidney of rats. E2 copreservation exerts protective activity against IRI\u2010induced pro\u2010inflammatory and proapoptotic effects in kidneys during organ preservation time and improves transplanted kidney function.<\/jats:p>","DOI":"10.1111\/aor.14038","type":"journal-article","created":{"date-parts":[[2021,7,12]],"date-time":"2021-07-12T18:56:24Z","timestamp":1626116184000},"page":"219-228","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Protective effect of estradiol copreservation against kidney ischemia\u2010reperfusion injury"],"prefix":"10.1111","volume":"46","author":[{"given":"Dawei","family":"Zhou","sequence":"first","affiliation":[{"name":"Hubei Key Laboratory of Medical Technology on Transplantation, Institute of Hepatobiliary Diseases of Wuhan University, Transplant Center of Wuhan University Zhongnan Hospital of Wuhan University  Wuhan P.R. 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China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yanfeng","family":"Wang","sequence":"additional","affiliation":[{"name":"Hubei Key Laboratory of Medical Technology on Transplantation, Institute of Hepatobiliary Diseases of Wuhan University, Transplant Center of Wuhan University Zhongnan Hospital of Wuhan University  Wuhan P.R. 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