{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T07:06:27Z","timestamp":1792652787392,"version":"4.5.0"},"reference-count":24,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2021,8,4]],"date-time":"2021-08-04T00:00:00Z","timestamp":1628035200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100004587","name":"Instituto de Salud Carlos III","doi-asserted-by":"publisher","award":["PI16\u201001315"],"award-info":[{"award-number":["PI16\u201001315"]}],"id":[{"id":"10.13039\/501100004587","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Artificial Organs"],"published-print":{"date-parts":[[2021,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>In tracheal replacement transplantation, prelamination is a critical stage. Nowadays, the most widely used prelamination technique is the prethoracic fascia flap with lateral thoracic artery. We propose a flap based on the internal thoracic artery, which allows a relatively non\u2010aggressive double organ implant, and we have tested its efficacy in decellularized tracheas. Tracheas of albino New Zealand rabbits were decellularized following a protocol that uses detergents and cryogenization, sterilized with 1kGy gamma radiation, and tutorized with a stent. Bilateral pedicled flaps made of pectoral fascia and a muscular component were harvested through a longitudinal 3\u2010cm central thoracic incision, wrapping the tracheas with them in 16 rabbits, remaining them implanted for 2, 4, 8, and 12\u00a0weeks. The tracheas were then studied histologically using standard stainings plus immunohistochemistry (CD31). The models were adjusted with Bayesian statistics using ordinal regression; results as odds ratios and credibility intervals. All analysis were performed using R software. Acute inflammatory cell invasion was observed at 2\u00a0weeks, which almost disappeared at week 8 after implant. Only macrophages and giant cells increased between Weeks 8 and 12 (OR 10.487, CI [1.603\u201097.327]). The cartilage maintained its structure, with slight signs of ischemia in a few cases. New CD31\u2010positive vessels were observed from Week 2 and increasing thereafter, reaching a maximum peak at Week 8. We propose a bilateral implant technique that is viable and effective as a prelamination option for two concurrent tracheas, achieving perfect vascularization and integration of the organ with hardly any inflammatory response in the medium or long term.<\/jats:p>","DOI":"10.1111\/aor.14043","type":"journal-article","created":{"date-parts":[[2021,7,26]],"date-time":"2021-07-26T18:36:27Z","timestamp":1627324587000},"page":"1491-1500","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Decellularized tracheal prelamination implant: A proposed bilateral double organ technique"],"prefix":"10.1111","volume":"45","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5529-9615","authenticated-orcid":false,"given":"N\u00e9stor J.","family":"Mart\u00ednez\u2010Hern\u00e1ndez","sequence":"first","affiliation":[{"name":"Thoracic Surgery Department Hospital Universitari de la Ribera Alzira Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana","family":"D\u00edaz\u2010Cuevas","sequence":"additional","affiliation":[{"name":"INCLIVA Universitat de Val\u00e8ncia Val\u00e8ncia Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lara","family":"Mili\u00e1n\u2010Medina","sequence":"additional","affiliation":[{"name":"Pathology Department Medicine and Odontology Faculty Universitat de Val\u00e8ncia Val\u00e8ncia Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mar\u00eda","family":"Sancho\u2010Tello","sequence":"additional","affiliation":[{"name":"INCLIVA Universitat de Val\u00e8ncia Val\u00e8ncia Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joan","family":"Rosell\u00f3\u2010Ferrando","sequence":"additional","affiliation":[{"name":"Hospital General Universitari de Val\u00e8ncia Val\u00e8ncia Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alfonso","family":"Morcillo\u2010Aixel\u00e1","sequence":"additional","affiliation":[{"name":"Thoracic Surgery Department Hospital Universitari i Polit\u00e8cnic la Fe Val\u00e8ncia Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jos\u00e9 Luis","family":"Campo\u2010Ca\u00f1averal","sequence":"additional","affiliation":[{"name":"Thoracic Surgery Department Hospital Universitario Puerta de Hierro Madrid Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amparo","family":"Roig\u2010Bataller","sequence":"additional","affiliation":[{"name":"Thoracic Surgery Department Hospital Universitari de la Ribera Alzira Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manuel","family":"Mata\u2010Roig","sequence":"additional","affiliation":[{"name":"Pathology Department Medicine and Odontology Faculty Universitat de Val\u00e8ncia Val\u00e8ncia Spain"},{"name":"Networking Research Center on Respiratory Diseases (CIBERER) ISCIII Madrid Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2021,8,4]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.athoracsur.2011.02.071"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.21037\/acs.2018.03.01"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-5223(19)42969-3"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.21037\/acs.2018.01.14"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0003-4975(02)03564-6"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/micr.1920150903"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1288\/00005537-199406000-00014"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa0810653"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMc1315273"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(09)60429-3"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-5223(95)70105-2"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.20517\/2347-9264.2015.117"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1093\/ejcts\/ezu444"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1093\/ejcts\/ezu133"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1093\/icvts\/ivs422"},{"key":"e_1_2_9_17_1","volume-title":"Le replacement circonf\u00e9rentiel \u00e9tendu de la trach\u00e9e. Etude clinique et exp\u00e9rimentale","author":"Wurtz A","year":"2014"},{"key":"e_1_2_9_18_1","volume-title":"The 3Rs | NC3Rs","author":"National Center for the Replacement Refinement & Reduction of Animals in Research","year":"2019"},{"key":"e_1_2_9_19_1","first-page":"391398821100891","article-title":"Optimization of a decellularization protocol of porcine tracheas. Long\u2010term effects of cryopreservation. A histological study","author":"Milian L","year":"2021","journal-title":"Int J Artif Organs"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1515\/fv-2017-0014"},{"key":"e_1_2_9_21_1","unstructured":"AENOR.UNE\u2010EN ISO 10993\u20106:2007. 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