{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T17:13:05Z","timestamp":1789146785947,"version":"build-2803163510"},"reference-count":41,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2021,10,31]],"date-time":"2021-10-31T00:00:00Z","timestamp":1635638400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/100016061","name":"Stichting de Drie Lichten","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100016061","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Artificial Organs"],"published-print":{"date-parts":[[2022,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>There is a continued interest in ex situ heart perfusion as an alternative strategy for donor heart preservation. We hypothesize that oxygenated machine perfusion of donor hearts at a temperature that avoids both normothermia and deep hypothermia offers adequate and safe preservation.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Cardioplegia\u2010arrested porcine donor hearts were randomly assigned to six hours of preservation using cold storage (CS, <jats:italic>n<\/jats:italic>\u00a0=\u00a05) or machine perfusion using an oxygenated acellular perfusate at 21\u00b0C (MP, <jats:italic>n<\/jats:italic>\u00a0=\u00a05). Subsequently, all grafts were evaluated using the Langendorff method for 120\u00a0min. Metabolic parameters and histology were analyzed. Systolic function was assessed by contractility and elastance. Diastolic function was assessed by lusitropy and stiffness.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>For both groups, in vivo baseline and post\u2010Langendorff biopsies were comparable, as were lactate difference and myocardial oxygen consumption. Injury markers gradually increased and were comparable. Significant weight gain was seen in MP (<jats:italic>p<\/jats:italic>\u00a0=\u00a00.008). Diastolic function was not impaired in MP, and lusitropy was superior from 30\u00a0min up to 90\u00a0min of reperfusion. Contractility was superior in MP during the first hour of evaluation.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>We conclude that the initial functional outcome of MP\u2010preserved hearts was transiently superior compared to CS, with no histological injury post\u2010Langendorff. Our machine perfusion strategy could offer feasible and safe storage of hearts prior to transplantation. Future studies are warranted for further optimization.<\/jats:p><\/jats:sec>","DOI":"10.1111\/aor.14085","type":"journal-article","created":{"date-parts":[[2021,10,11]],"date-time":"2021-10-11T12:39:22Z","timestamp":1633955962000},"page":"246-258","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Oxygenated machine perfusion at room temperature as an alternative for static cold storage in porcine donor hearts"],"prefix":"10.1111","volume":"46","author":[{"given":"Vincent","family":"van Suylen","sequence":"first","affiliation":[{"name":"Department of Cardiothoracic Surgery University Medical Center Groningen University of Groningen  Groningen the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5405-1343","authenticated-orcid":false,"given":"Katrien","family":"Vandendriessche","sequence":"additional","affiliation":[{"name":"Department of Cardiovascular 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