{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,8]],"date-time":"2026-08-08T02:45:07Z","timestamp":1786157107465,"version":"build-2736575974"},"reference-count":33,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2022,1,22]],"date-time":"2022-01-22T00:00:00Z","timestamp":1642809600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2022,1,22]],"date-time":"2022-01-22T00:00:00Z","timestamp":1642809600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Artificial Organs"],"published-print":{"date-parts":[[2022,6]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Artificial oxygen carriers (HbV) can treat hemorrhagic shock with lethal arrhythmias (VT\/VF). No reports exist on subacute HbV\u2019s effects.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Acute and subacute resuscitation effects with anti\u2010arrhythmogenesis of HbV were studied in 85% blood exchange rat model (85%\u2010Model). Lethal 85%\u2010Model was created by bone marrow transfusion and femoral artery bleeding in 80 SD rats in HbV\u2010administered group (HbV\u2010group), washed erythrocyte\u2010administered group (wRBC\u2010group), and 5% albumin\u2010administered group (ALB\u2010group). Survival rates, anti\u2010arrhythmic efficacy by optical mapping system (OMP) with electrophysiological study (EPS) in Langendorff heart, cardiac autonomic activity by heart rate variability (HRV) and ventricular arrhythmias by 24\u2010h electrocardiogram telemetry monitoring (24\u00a0h\u2010ECG) in awake, and left ventricular function by echocardiography (left ventricular ejection fraction [LVEF]) were measured.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      All rats in HbV\u2010 and wRBC\u2010groups survived for 4 weeks, whereas no rats in ALB\u2010group. HbV and wRBC acutely suppressed VT\/VF in Langendorff heart through ameliorating action potential duration dispersion (APDd) analyzed by OMP with EPS. For subacute analysis, 50% blood exchange by 5% albumin was used (ALB\u2010group 50). Subacute salutary effect on APDd and VT\/VF inducibility was confirmed in HbV\u2010 and wRBC\u2010groups. 24\u00a0h\u2010ECG showed that HbV and wRBC suppressed none\u2010sustained ventricular tachycardia (NSVT) and sympathetic component of HRV (LF\/HF) with preserved LVEF (HbV\u2010group, wRBC\u2010group vs. ALB\u2010group 50; NSVT numbers\/days, 0.5\u00a0\u00b1\u00a00.3, 0.4\u00a0\u00b1\u00a00.3 vs. 3.9\u00a0\u00b1\u00a01.2*; LF\/HF, 1.1\u00a0\u00b1\u00a00.2, 0.8\u00a0\u00b1\u00a00.2 vs. 3.5\u00a0\u00b1\u00a01.0*; LVEF, 84\u00a0\u00b1\u00a05, 83\u00a0\u00b1\u00a04, vs. 77\u00a0\u00b1\u00a04%*; *\n                      <jats:italic>p<\/jats:italic>\n                      \u00a0&lt;\u00a00.05).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Collectively, HbV has sustained antiarrhythmic effect in subacute 85%\u2010Model by ameliorating electrical remodeling and improving arrhythmogenic modifying factors (HRV and LVEF). These findings are useful in now continuing clinical trials of HbV.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/aor.14170","type":"journal-article","created":{"date-parts":[[2022,1,10]],"date-time":"2022-01-10T10:44:47Z","timestamp":1641811487000},"page":"1107-1121","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Intraosseous infusion of liposome\u2010encapsulated hemoglobin (HbV) acutely prevents hemorrhagic anemia\u2010induced lethal arrhythmias, and its efficacy persists with preventing proarrhythmic side effects in the subacute phase of severe hemodilution model"],"prefix":"10.1111","volume":"46","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7889-6260","authenticated-orcid":false,"given":"Bonpei","family":"Takase","sequence":"first","affiliation":[{"name":"Department of Intensive Care Medicine National Defense Medical College  Tokorozawa Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuko","family":"Higashimura","sequence":"additional","affiliation":[{"name":"Department of Intensive Care Medicine National Defense Medical College  Tokorozawa Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haruka","family":"Asahina","sequence":"additional","affiliation":[{"name":"Department of Critical Care Medicine National Defense Medical College  Tokorozawa Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nobuyuki","family":"Masaki","sequence":"additional","affiliation":[{"name":"Department of Intensive Care Medicine National Defense Medical College  Tokorozawa Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manabu","family":"Kinoshita","sequence":"additional","affiliation":[{"name":"Department of Immunology and Microbiology National Defense Medical College  Tokorozawa Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiromi","family":"Sakai","sequence":"additional","affiliation":[{"name":"Department of Chemistry School of Medicine Nara Medical University  Kashihara Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,1,22]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1021\/bc960069p"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.00307.2003"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1537\u20102995.2003.00341.x"},{"key":"e_1_2_7_5_1","first-page":"505","article-title":"A phase I single blind clinical trial of a new oxygen transport agent (MP4), human hemoglobin modified with maleimide\u2010activated polyethylene glycol","volume":"90","author":"Bj\u00f6rkholm 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