{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,14]],"date-time":"2025-05-14T00:41:19Z","timestamp":1747183279369,"version":"3.40.5"},"reference-count":24,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2023,12,22]],"date-time":"2023-12-22T00:00:00Z","timestamp":1703203200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Artificial Organs"],"published-print":{"date-parts":[[2024,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Neutrophils take part in the innate immune response, phagocytosis, and pro\u2010inflammatory cytokine release. The phagocytic capacity of circulating neutrophils in patients on continuous flow (CF) ventricular assist device (VAD) has not been well studied.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Blood samples from 14 patients undergoing CF\u2010VAD implantation were collected and analyzed preoperatively (at baseline) and on postoperative days (POD) 3, 7, 14, and 28. Flow cytometry was used to assess the surface expression levels of CD62L, CD162, and macrophage antigen\u20101 (MAC\u20101) and neutrophil phagocytic capacity. Interleukin 1 (IL1), IL6, IL8, TNF\u2010\u03b1, neutrophil elastase, and myeloperoxidase in plasma were measured using enzyme\u2010linked immunosorbent assays.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Among the 14 patients, seven patients had preoperative bridge device support. Relative to baseline, patients with no bridge device had elevated leukocyte count and neutrophil elastase by POD3 which normalized by POD7. Neutrophil activation level, IL6, IL8, and TNF\u2010\u03b1 increased by POD3 and sustained elevated levels for 7\u201314\u2009days postoperatively. Elevated neutrophil phagocytic capacity persisted even until POD28. Similar patterns were observed in patients on a preoperative bridge device.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Neutrophil activation and phagocytic capacity increased in response to VAD support, while inflammatory cytokines remain elevated for up to 2\u2009weeks postoperatively. These findings may indicate that VAD implantation elicits circulating neutrophils to an abnormal preemptive phagocytotic phenotype.<\/jats:p><\/jats:sec>","DOI":"10.1111\/aor.14693","type":"journal-article","created":{"date-parts":[[2023,12,22]],"date-time":"2023-12-22T14:11:20Z","timestamp":1703254280000},"page":"636-645","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Increased phagocytosis capacity of circulating neutrophils in patients on continuous flow ventricular assist device support"],"prefix":"10.1111","volume":"48","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2568-3330","authenticated-orcid":false,"given":"Morcos A.","family":"Awad","sequence":"first","affiliation":[{"name":"Department of Surgery University of Maryland School of Medicine  Baltimore Maryland USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wenji","family":"Sun","sequence":"additional","affiliation":[{"name":"Department of Surgery University of Maryland School of Medicine  Baltimore Maryland USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dong","family":"Han","sequence":"additional","affiliation":[{"name":"Department of Surgery University of Maryland School of Medicine  Baltimore Maryland USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bartley P.","family":"Griffith","sequence":"additional","affiliation":[{"name":"Department of Surgery University of Maryland School of Medicine  Baltimore Maryland USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0807-7195","authenticated-orcid":false,"given":"Zhongjun\u00a0J.","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Surgery University of Maryland School of Medicine  Baltimore Maryland USA"},{"name":"Fischell Department of Bioengineering, A. James Clark School of Engineering University of Maryland  College Park Maryland USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2023,12,22]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/nri1375"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1177\/0391398818817326"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(06)69005-3"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(94)90337-9"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2022.910908"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.abb.2018.01.004"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1510\/icvts.2010.232603"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1177\/0391398818784276"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1097\/MAT.0000000000000971"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cardfail.2019.01.005"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/aor.12200"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1080\/09537104.2020.1859101"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/aor.13471"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cardfail.2019.09.013"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.healun.2015.03.004"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bcmd.2016.04.003"},{"key":"e_1_2_9_18_1","first-page":"406","article-title":"In vivo evaluation of the HeartWare centrifugal ventricular assist device","volume":"34","author":"Tuzun E","year":"2007","journal-title":"Tex Heart Inst J"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1111\/aor.14068"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1111\/aor.13874"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1111\/aor.13068"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1111\/aor.13409"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.110463197"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.86.1.e13"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1097\/00002480-200105000-00023"}],"container-title":["Artificial Organs"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/aor.14693","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,5,20]],"date-time":"2024-05-20T09:20:52Z","timestamp":1716196852000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/aor.14693"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,12,22]]},"references-count":24,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2024,6]]}},"alternative-id":["10.1111\/aor.14693"],"URL":"https:\/\/doi.org\/10.1111\/aor.14693","archive":["Portico"],"relation":{},"ISSN":["0160-564X","1525-1594"],"issn-type":[{"type":"print","value":"0160-564X"},{"type":"electronic","value":"1525-1594"}],"subject":[],"published":{"date-parts":[[2023,12,22]]},"assertion":[{"value":"2023-08-09","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-12-06","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-12-22","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}