{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,25]],"date-time":"2026-08-25T21:08:56Z","timestamp":1787692136054,"version":"build-2784847793"},"reference-count":40,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2024,2,21]],"date-time":"2024-02-21T00:00:00Z","timestamp":1708473600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["52025051"],"award-info":[{"award-number":["52025051"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Artificial Organs"],"published-print":{"date-parts":[[2024,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Thromboembolism, which leads to pulmonary embolism and ischemic stroke, remains one of the main causes of death. Ultrasound\u2010assisted thrombolysis (UAT) is an effective thrombolytic method. However, further studies are required to elucidate the mechanism of ultrasound on arterial and venous thrombi.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We employed the blood\u2010on\u2010a\u2010chip technology to simulate thrombus formation in coronary stenosis and deep vein valves. Subsequently, UAT was conducted on the chip to assess the impact of ultrasound on thrombolysis under varying flow conditions. Real\u2010time fluorescence was used to assess thrombolysis and drug penetration. Finally, scanning electron microscopy and immunofluorescence were used to determine the effect of ultrasound on fibrinolysis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The study revealed that UAT enhanced the thrombolytic rate by 40% in the coronary stenosis chip and by 10% in the deep venous valves chip. This enhancement is attributed to the disruption of crosslinked fibrin fibers by ultrasound, leading to increased urokinase diffusion within the thrombus and accumulation of plasminogen on the fibrinogen \u03b1 chain. Moreover, the acceleration of the dissolution rate of thrombi in the venous valve chip by ultrasound was not as significant as that in the coronary stenosis chip.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>These findings highlight the differential impact of ultrasound on thrombolysis under various flow conditions and emphasize the valuable role of the blood\u2010on\u2010a\u2010chip technology in exploring thrombolysis mechanisms.<\/jats:p><\/jats:sec>","DOI":"10.1111\/aor.14731","type":"journal-article","created":{"date-parts":[[2024,2,21]],"date-time":"2024-02-21T12:24:40Z","timestamp":1708518280000},"page":"734-742","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["The effect of ultrasound\u2010assisted thrombolysis studied in blood\u2010on\u2010a\u2010chip"],"prefix":"10.1111","volume":"48","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0576-5144","authenticated-orcid":false,"given":"Yan","family":"Li","sequence":"first","affiliation":[{"name":"State Key Laboratory of Tribology in Advanced Equipment Tsinghua University  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3485-4253","authenticated-orcid":false,"given":"Yongjian","family":"Li","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Tribology in Advanced Equipment Tsinghua University  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haosheng","family":"Chen","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Tribology in Advanced Equipment Tsinghua University  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,2,21]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(16)30514-1"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1161\/ATVBAHA.116.308622"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2015-08-662650"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrcardio.2017.206"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1525-1594.2010.01021.x"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ultras.2008.03.010"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-020-69202-9"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.3389\/fbioe.2022.923365"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.3390\/jcm12124006"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.3233\/CH-211134"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-319-22536-4_19"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0049-3848(00)00319-4"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1121\/1.4805712"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ultrasmedbio.2014.08.018"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-0038-1657688"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11239-018-1629-y"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa041175"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1093\/eurheartj\/ehu029"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.2310\/6670.2009.00063"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcin.2015.04.020"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1378\/chest.15-0119"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1525-1594.2010.01114.x"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1111\/aor.14653"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1161\/ATVBAHA.118.311178"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm.1955"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1039\/D0LC00287A"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1038\/s43246-020-00066-2"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1063\/5.0149884"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2261-14-131"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1177\/1358863X16645853"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcmg.2021.07.028"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsnano.1c07830"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.1109\/T-SU.1985.31608"},{"key":"e_1_2_8_35_1","doi-asserted-by":"crossref","unstructured":"ThouvenotA PoeppingT PetersTM ChenECS.Characterization of various tissue mimicking materials for medical ultrasound imaging\u201d Proc. SPIE 9783 Medical Imaging 2016: Physics of Medical Imaging 97835E.","DOI":"10.1117\/12.2218160"},{"key":"e_1_2_8_36_1","doi-asserted-by":"publisher","DOI":"10.1088\/0031-9155\/52\/20\/N02"},{"key":"e_1_2_8_37_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1538-7836.2005.01365.x"},{"key":"e_1_2_8_38_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms10176"},{"key":"e_1_2_8_39_1","doi-asserted-by":"publisher","DOI":"10.1039\/D1LC00276G"},{"key":"e_1_2_8_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pbiomolbio.2006.07.024"},{"key":"e_1_2_8_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actbio.2016.11.049"}],"container-title":["Artificial Organs"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/aor.14731","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,6,11]],"date-time":"2024-06-11T19:01:06Z","timestamp":1718132466000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/aor.14731"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,2,21]]},"references-count":40,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2024,7]]}},"alternative-id":["10.1111\/aor.14731"],"URL":"https:\/\/doi.org\/10.1111\/aor.14731","archive":["Portico"],"relation":{},"ISSN":["0160-564X","1525-1594"],"issn-type":[{"value":"0160-564X","type":"print"},{"value":"1525-1594","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,2,21]]},"assertion":[{"value":"2023-11-14","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-02-07","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-02-21","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}