{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T13:53:45Z","timestamp":1792072425256,"version":"4.4.0"},"reference-count":28,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2024,11,20]],"date-time":"2024-11-20T00:00:00Z","timestamp":1732060800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Artificial Organs"],"published-print":{"date-parts":[[2025,3]]},"abstract":"Abstract<\/jats:title>\n \n Background<\/jats:title>\n The interleukin 1 (IL\u20101) cytokine group plays a key role in sterile inflammation and may be an important target for transplant\u2010related renal injury. This study examined the effects of anakinra, a non\u2010specific IL\u20101 receptor antagonist, administered during normothermic machine perfusion (NMP) of porcine kidneys.<\/jats:p>\n <\/jats:sec>\n \n Method<\/jats:title>\n \n Paired porcine kidneys (\n n<\/jats:italic>\n \u2009=\u20095 pairs) underwent 15\u2009min of warm ischemia plus 2\u2009h of static cold storage in ice. Kidneys were then perfused with autologous whole blood using an ex\u00a0vivo NMP platform. Kidneys were randomly allocated to receive anakinra or vehicle administered at the start of NMP. Cortical biopsies were collected at baseline before ischemic injury and at the end of NMP. Functional parameters were recorded and calculated, and inflammatory markers were measured by qPCR and ELISA techniques.\n <\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n \n During NMP, there were no statistically significant differences in renal blood flow, urine output, creatinine clearance or fractional excretion of sodium in the anakinra and control groups. The administration of anakinra significantly downregulated transcriptional expression of IL\u20106, Fas ligand and intercellular adhesion molecule 1 (\n p<\/jats:italic>\n \u2009=\u20090.029, 0.029, 0.028, respectively).\n <\/jats:p>\n <\/jats:sec>\n \n Conclusion<\/jats:title>\n Anakinra, an IL\u20101 receptor blocker, successfully attenuated the downstream inflammatory and immune\u2010mediated response within the kidney during NMP.<\/jats:p>\n <\/jats:sec>","DOI":"10.1111\/aor.14909","type":"journal-article","created":{"date-parts":[[2024,11,20]],"date-time":"2024-11-20T08:20:04Z","timestamp":1732090804000},"page":"451-459","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["IL<\/scp>\n \u20101 receptor antagonist anakinra downregulates inflammatory cytokines during renal normothermic machine perfusion: Preliminary results"],"prefix":"10.1111","volume":"49","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8039-143X","authenticated-orcid":false,"given":"Sarah A.","family":"Hosgood","sequence":"first","affiliation":[{"name":"Department of Surgery Addenbrooke's Hospital, University of Cambridge Cambridge UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tom","family":"Moore","sequence":"additional","affiliation":[{"name":"Department of Surgery Addenbrooke's Hospital, University of Cambridge Cambridge UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alex","family":"Walker","sequence":"additional","affiliation":[{"name":"Department of Surgery Addenbrooke's Hospital, University of Cambridge Cambridge UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael L.","family":"Nicholson","sequence":"additional","affiliation":[{"name":"Department of Surgery Addenbrooke's Hospital, University of Cambridge Cambridge 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