{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,12]],"date-time":"2025-10-12T02:27:33Z","timestamp":1760236053269,"version":"build-2065373602"},"reference-count":38,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2024,12,6]],"date-time":"2024-12-06T00:00:00Z","timestamp":1733443200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100006005","name":"Agence de la Biom\u00e9decine","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100006005","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100016396","name":"Association Fran\u00e7aise d'Urologie","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100016396","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Artificial Organs"],"published-print":{"date-parts":[[2025,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Preclinical and clinical studies have demonstrated the advantages of oxygenated hypothermic machine perfusion (HMPO<jats:sub>2<\/jats:sub>) during kidney preservation. However, the optimal oxygenation levels during HMPO<jats:sub>2<\/jats:sub> remain undetermined. The aim of this study was to compare different levels of oxygenation (0%, 21%, 50%, and 100%) during 22 h of active oxygenated HMP (HMPO<jats:sub>2<\/jats:sub>) using oxygen preloading by bubbling oxygenation in the preservation solution and continuous surface oxygenation during MP in a porcine DCD model.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>After 60\u2009min of warm ischemia time, both kidneys of an 80\u2009kg pig were procured and randomized to one of the following groups: (1) 22\u2010h static cold storage (<jats:italic>n<\/jats:italic>\u2009=\u20096), (2) 22\u2010h HMP without active oxygenation (<jats:italic>n<\/jats:italic>\u2009=\u20096), (3) 22\u2010h HMPO<jats:sub>2<\/jats:sub> 21% O<jats:sub>2<\/jats:sub> (<jats:italic>n<\/jats:italic>\u2009=\u20096), (4) 22\u2010h HMPO<jats:sub>2<\/jats:sub> 50% O<jats:sub>2<\/jats:sub> (<jats:italic>n<\/jats:italic>\u2009=\u20096), and (5) 22\u2010h HMPO<jats:sub>2<\/jats:sub> 100% O<jats:sub>2<\/jats:sub> (<jats:italic>n<\/jats:italic>\u2009=\u20096). The primary outcome measure was to compare the different oxygen levels among the different groups assessed by cortex kidney tissue oxygen partial pressures (tpO<jats:sub>2<\/jats:sub>).<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Continuous HMPO<jats:sub>2<\/jats:sub> resulted in a significant modification of cortex kidney tpO<jats:sub>2<\/jats:sub>. In addition, tpO<jats:sub>2<\/jats:sub> was dependent on the percentage of oxygenation. One hundred percent oxygen resulted in a significantly higher tpO<jats:sub>2<\/jats:sub> compared to all other study groups. In line with that, ATP resynthesis was significantly higher in the HMPO<jats:sub>2<\/jats:sub> 100% group.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>This study demonstrates that continuous HMPO<jats:sub>2<\/jats:sub> results in a significant modification of tpO<jats:sub>2<\/jats:sub> compared with SCS, and the degree of tpO<jats:sub>2<\/jats:sub> is positively correlated with the percentage of active oxygenation during HMP. Metabolic profile analyses (ATP resynthesis) suggest that the aerobic mechanism is better supported with higher oxygen levels (50% and 100% oxygenation).<\/jats:p><\/jats:sec>","DOI":"10.1111\/aor.14916","type":"journal-article","created":{"date-parts":[[2024,12,6]],"date-time":"2024-12-06T07:00:10Z","timestamp":1733468410000},"page":"600-614","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Cortex kidney tissue partial oxygen pressure depends on percentage of active oxygenation during oxygenated hypothermic machine perfusion"],"prefix":"10.1111","volume":"49","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3601-9339","authenticated-orcid":false,"given":"Thomas","family":"Prudhomme","sequence":"first","affiliation":[{"name":"Nantes Universit\u00e9 CHU Nantes, INSERM, Centre for Research in Transplantation and Translational Immunology  Nantes 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Centre for Research in Transplantation and Translational Immunology  Nantes France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J\u00e9r\u00e9my","family":"Hervouet","sequence":"additional","affiliation":[{"name":"Nantes Universit\u00e9 CHU Nantes, INSERM, Centre for Research in Transplantation and Translational Immunology  Nantes France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David","family":"Minault","sequence":"additional","affiliation":[{"name":"Nantes Universit\u00e9 CHU Nantes, INSERM, Centre for Research in Transplantation and Translational Immunology  Nantes France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gilles","family":"Blancho","sequence":"additional","affiliation":[{"name":"Nantes Universit\u00e9 CHU Nantes, INSERM, Centre for Research in Transplantation and Translational Immunology  Nantes 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