{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,4,25]],"date-time":"2025-04-25T04:17:24Z","timestamp":1745554644622,"version":"3.40.4"},"reference-count":37,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2025,1,16]],"date-time":"2025-01-16T00:00:00Z","timestamp":1736985600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100012629","name":"Japan Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100012629","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Artificial Organs"],"published-print":{"date-parts":[[2025,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Impairment of the visceral pleura following thoracic surgery often leads to air leaks and intrathoracic adhesions. For preventing such complications, mesothelial cell proliferation at the pleural defects can be effective. To develop new materials for pleural defects restoration, we constructed a hybrid artificial pleural tissue (H\u2010APLT) combining polyglycolic acid (PGA) nanofiber sheets with a three\u2010dimensional culture of mesothelial cells and fibroblasts and evaluated its therapeutic efficacy in a rat pleural defect model.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>After rat lungs were harvested, pleural mesothelial cells and lung fibroblasts were cultured separately. To construct H\u2010APLT, the cells were then coated with multiple layers of fibronectin and gelatin, followed by a single layer of mesothelial cells on top of multiple layers of fibroblasts accumulated onto a collagen\u2010coated PGA nanofiber sheet. Left lateral thoracotomy was performed, and H\u2010APLTs were transplanted into a rat model with pleural defects (<jats:italic>N<\/jats:italic>\u2009=\u20098). After 2\u201312\u2009weeks of transplantation, lung resection and histological analyses were performed.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>H\u2010APLTs exhibited a pleural structure with a highly integrated mesothelial layer in\u00a0vitro. After transplantation, all eight rats survived until sacrifice. At 12\u2009weeks post\u2010transplantation, the mesothelial layer on the lung surface was observed to be without defects with no intrathoracic adhesions detected.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Successful isogenic engraftment of H\u2010APLTs was achieved in a rat model of pleural defects. The combination of accumulated fibroblasts and collagen\u2010coated PGA nanofiber sheets contributed to the maintenance of the mesothelial layer's structure and function, potentially preventing air leaks and intrathoracic adhesions.<\/jats:p><\/jats:sec>","DOI":"10.1111\/aor.14947","type":"journal-article","created":{"date-parts":[[2025,1,16]],"date-time":"2025-01-16T19:05:23Z","timestamp":1737054323000},"page":"778-789","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Isogenic Transplantation of Hybrid Artificial Pleural Tissue Consisting of Rat Cells and Polyglycolic Acid Nanofiber Sheet Induces Restoration of Mesothelial Defects in Rat Model"],"prefix":"10.1111","volume":"49","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3218-3895","authenticated-orcid":false,"given":"Kengo","family":"Tani","sequence":"first","affiliation":[{"name":"Department of Thoracic and Cardiovascular Surgery Hirosaki University Graduate School of Medicine  Hirosaki Aomori Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daisuke","family":"Kimura","sequence":"additional","affiliation":[{"name":"Department of Thoracic and Cardiovascular Surgery Hirosaki University Graduate School of Medicine  Hirosaki Aomori Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yoshiya","family":"Asano","sequence":"additional","affiliation":[{"name":"Department of Neuroanatomy, Cell Biology and Histology Hirosaki University Graduate School of Medicine  Hirosaki Aomori Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cheng\u2010Yang","family":"Song","sequence":"additional","affiliation":[{"name":"Department of Thoracic and Cardiovascular Surgery Hirosaki University Graduate School of Medicine  Hirosaki Aomori Japan"},{"name":"Department of Thoracic Surgery Fourth Affiliated Hospital of China Medical University  Shenyang Liaoning China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hiroshi","family":"Shimoda","sequence":"additional","affiliation":[{"name":"Department of Neuroanatomy, Cell Biology and Histology Hirosaki University Graduate School of Medicine  Hirosaki Aomori Japan"},{"name":"Department of Anatomical Science Hirosaki University Graduate School of Medicine  Hirosaki Aomori Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masahito","family":"Minakawa","sequence":"additional","affiliation":[{"name":"Department of Thoracic and Cardiovascular Surgery Hirosaki University Graduate School of Medicine  Hirosaki Aomori 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