{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,10]],"date-time":"2026-10-10T02:50:38Z","timestamp":1791600638332,"version":"4.3.4"},"reference-count":14,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2025,4,3]],"date-time":"2025-04-03T00:00:00Z","timestamp":1743638400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,4,3]],"date-time":"2025-04-03T00:00:00Z","timestamp":1743638400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Artificial Organs"],"published-print":{"date-parts":[[2025,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Continuous renal replacement therapy (CRRT) is increasingly employed in pediatric intensive care units (PICUs) for managing both renal and non\u2010renal conditions, including sepsis through cytokine removal. Allogeneic hematopoietic stem cell transplantation (allo\u2010HSCT) is a critical treatment for inherited bone marrow syndromes but poses challenges when performed alongside extracorporeal therapies like CRRT. This case explores the interaction between hematopoietic stem cells and AN69 membranes during CRRT in a pediatric patient undergoing allo\u2010HSCT amid septic shock.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>A 10\u2010year\u2010old boy with bone marrow failure of unknown etiology and chronic kidney disease underwent allo\u2010HSCT from a fully matched sibling donor. The patient developed septic shock following a reduced\u2010intensity conditioning regimen and was managed with continuous venovenous hemodiafiltration using an AN69 membrane. During the allo\u2010HSCT infusion, the CRRT membrane became clogged, prompting immunohistochemical analysis of the AN69 membrane to investigate potential HSC entrapment. CD34\u2010positive cells and fibronectin expression on the membrane were assessed using staining protocols.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Despite severe septic shock and potential HSC entrapment in the AN69 membrane, the patient achieved full donor chimerism with 97% hematopoietic engraftment by day nineteen post\u2010HSCT. Immunohistochemical analysis revealed the presence of CD34\u2010positive HSCs and strong fibronectin staining on the clogged AN69 membrane, indicating significant interaction and entrapment of stem cells.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Allo\u2010HSCT can be performed successfully in pediatric patients with severe septic shock requiring CRRT, offering a potentially life\u2010saving option in urgent transplant situations. This case highlights that although HSPCs can be entrapped on AN69 membranes, engraftment was not compromised. Our findings provide novel insights into HSPC interactions with AN69 membranes during CRRT, and further studies are needed to quantify stem cell loss and explore its clinical implications.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/aor.15004","type":"journal-article","created":{"date-parts":[[2025,4,4]],"date-time":"2025-04-04T21:59:51Z","timestamp":1743803991000},"page":"1327-1331","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Evidence of Stem Cell Entrapment in\n                    <scp>AN69<\/scp>\n                    Membranes During Continuous Renal Replacement Therapy"],"prefix":"10.1111","volume":"49","author":[{"given":"Izzet Turkalp","family":"Akbasli","sequence":"first","affiliation":[{"name":"Department of Heart, Vascular, and Thoracic, Division of Cardiology and Cardiovascular Medicine Children's Institute, Cleveland Clinic  Cleveland Ohio USA"},{"name":"The Center for Life Support Practice and Research Hacettepe University  Ankara T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ozlem","family":"Saritas Nakib","sequence":"additional","affiliation":[{"name":"The Center for Life Support Practice and Research Hacettepe University  Ankara T\u00fcrkiye"},{"name":"Department of Pediatric Intensive Care Unit Birmingham Children's Hospital Steelhouse Ln  Birmingham UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Selman","family":"Kesici","sequence":"additional","affiliation":[{"name":"Department of Pediatric Intensive Care Medicine Life Support Center Hacettepe University  Ankara T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fatma Visal","family":"Okur","sequence":"additional","affiliation":[{"name":"Division of Pediatric Hematology and Bone Marrow Transplantation Unit, Department of Pediatrics, School of Medicine Hacettepe University  Ankara T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kadri Safak","family":"Gucer","sequence":"additional","affiliation":[{"name":"Faculty of Medicine, Division of Pediatric and Perinatal Pathology, Department of Pediatrics Hacettepe University  Ankara T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Benan","family":"Bayrakci","sequence":"additional","affiliation":[{"name":"Department of Pediatric Intensive Care Medicine Life Support Center Hacettepe University  Ankara T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,4,3]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1001\/jamanetworkopen.2022.46901"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1097\/PCC.0000000000002198"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2141.2009.07842.x"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00277-023-05406-z"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00508-011-1590-7"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/leu.2015.75"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.4103\/0972\u20105229.192062"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmed.2022.789623"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1525-1594.2003.07289.x"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13613-020-0648-y"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcrc.2019.07.002"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/pbc.24753"},{"key":"e_1_2_9_14_1","article-title":"Immuno\u2010Modulation of Hematopoietic Stem and Progenitor Cells in Inflammation","volume":"20","author":"Sezaki M.","year":"2020","journal-title":"Frontiers in Immunology"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1159\/000328961"}],"container-title":["Artificial Organs"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/aor.15004","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/aor.15004","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/aor.15004","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,10]],"date-time":"2026-10-10T02:43:42Z","timestamp":1791600222000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/aor.15004"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,4,3]]},"references-count":14,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2025,8]]}},"alternative-id":["10.1111\/aor.15004"],"URL":"https:\/\/doi.org\/10.1111\/aor.15004","archive":["Portico"],"relation":{},"ISSN":["0160-564X","1525-1594"],"issn-type":[{"value":"0160-564X","type":"print"},{"value":"1525-1594","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,4,3]]},"assertion":[{"value":"2024-11-06","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-03-25","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-04-03","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}