{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,13]],"date-time":"2026-03-13T15:56:07Z","timestamp":1773417367358,"version":"3.50.1"},"reference-count":26,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2025,6,9]],"date-time":"2025-06-09T00:00:00Z","timestamp":1749427200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100007225","name":"Ministry of Science and Technology","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100007225","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Artificial Organs"],"published-print":{"date-parts":[[2025,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Cardiovascular disease patients may find new hope in tissue engineering vascular grafts (TEVGs), which show promise as a substitute for small\u2010diameter grafts in bypass surgery. Decellularized and crosslinked arteries are good TEVGs because they have native tissue structure, mechanical stability, reduced immunogenicity, and biocompatibility. Currently, procedures for decellularization and crosslinking related to bioreactors are emergent due to creating a stable flow, reducing both time and chemical concentration\u2014factors affecting vascular structural degradation.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>In the bioreactor system, the arteries were decellularized with low\u2010concentration (0.3% or 0.5%) sodium dodecyl sulfate (SDS) before being crosslinked using N\u2010hydroxysuccinimide (NHS) and N\u2010(3\u2010dimethylaminopropyl)\u2010N\u2032\u2010ethylcarbodiimide hydrochloride (EDC). The samples were tested for mechanical properties, in\u00a0vitro cytotoxicity, histological analysis, degradation, and subcutaneous embedding for about 3 months to see how well the crosslinking and decellularization process worked.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>A perfusion bioreactor along with 0.5% SDS shows the efficiency in removing cells from the arteries compared to 0.3% SDS. Using crosslinking with EDC and NHS by bioreactor also improved their mechanical properties, lowered inflammatory reactions, and sped up the time it took for them to break down.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>The combination of SDS, EDC\/NHS, and a bioreactor may serve as an appropriate method for arterial decellularization and crosslinking. This research indicates that crosslinked decellularized grafts by bioreactor systems are a promising scaffold materials for bypass surgery or vascular repair.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/aor.15037","type":"journal-article","created":{"date-parts":[[2025,6,9]],"date-time":"2025-06-09T07:40:37Z","timestamp":1749454837000},"page":"1512-1520","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["The Decellularization and Crosslinking Efficacy of Perfusion Bioreactors in Small\u2010Diameter Porcine Carotid Artery Grafts"],"prefix":"10.1111","volume":"49","author":[{"given":"Nho Thuan","family":"Nguyen","sequence":"first","affiliation":[{"name":"Laboratory of Tissue Engineering and Biomedical Materials University of Science  Ho Chi Minh City Vietnam"},{"name":"Department of Physiology and Animal Biotechnology Faculty of Biology and Biotechnology, University of Science  Ho Chi Minh City Vietnam"},{"name":"Vietnam National University  Ho Chi Minh City Vietnam"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Binh Duc","family":"Vu","sequence":"additional","affiliation":[{"name":"National Institute of Hematology and Blood Transfusion  Vietnam"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thao Duy","family":"Huynh","sequence":"additional","affiliation":[{"name":"Pham Ngoc Thach University of Medicine  Ho Chi Minh City Vietnam"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thang Quoc","family":"Bui","sequence":"additional","affiliation":[{"name":"Cho ray Hospital  Ho Chi Minh City Vietnam"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5848-1124","authenticated-orcid":false,"given":"Ha Le Bao","family":"Tran","sequence":"additional","affiliation":[{"name":"Laboratory of Tissue Engineering and Biomedical Materials University of Science  Ho Chi Minh City Vietnam"},{"name":"Department of Physiology and Animal Biotechnology Faculty of Biology and Biotechnology, University of Science  Ho Chi Minh City Vietnam"},{"name":"Vietnam National University  Ho Chi Minh City Vietnam"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,6,9]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIR.0000000000001303"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.3390\/bioengineering7040160"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1089\/ten.tea.2008.0526"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1177\/0391398820975420"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10561-019-09792-5"},{"key":"e_1_2_9_7_1","article-title":"Decellularization and Recellularization Methodology for Human Saphenous Veins","volume":"137","author":"Kuna V. 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