{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,4]],"date-time":"2026-06-04T06:02:51Z","timestamp":1780552971843,"version":"3.54.1"},"reference-count":42,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2026,4,20]],"date-time":"2026-04-20T00:00:00Z","timestamp":1776643200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,4,20]],"date-time":"2026-04-20T00:00:00Z","timestamp":1776643200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Artificial Organs"],"published-print":{"date-parts":[[2026,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Ex vivo lung perfusion (EVLP) enables transplant physicians to assess and optimize donor lung quality, thereby expanding the donor pool and improving clinical outcomes. However, elevated hemolysis and lactate accumulation within the EVLP perfusate may compromise graft viability.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>The primary goal of this study was to evaluate the hemocompatibility of two cellular perfusates\u2014whole blood (WB) and leukocyte\u2010depleted blood (LD)\u2014using identical mock EVLP loops. Saline\u2010washed red blood cells (RBC) were included as an additional background reference perfusate. All perfusates were filtered and diluted to a hematocrit of 19.1%\u2009\u00b1\u20091.3% and circulated for 10\u2009h at 37\u00b0C with a flow rate of 2.7\u2009\u00b1\u20090.2\u2009L\/min.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Over the course of in\u00a0vitro blood circulation, the rates of increase in hemolysis and lactate levels were significantly higher in the WB group than in the LD and RBC groups (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.05), with no significant differences observed between LD and RBC. The normalized index of hemolysis at 10\u2009h was significantly greater in the WB group compared with LD (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.01) and RBC (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.05).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>These findings suggest that, in blood\u2010based ex\u00a0vivo perfusion, leukocyte depletion is associated with reduced hemolysis and lactate accumulation. In addition, a simple yet promising in\u00a0vitro blood loop approach can be used to evaluate perfusate quality and improve EVLP outcomes.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/aor.70138","type":"journal-article","created":{"date-parts":[[2026,4,21]],"date-time":"2026-04-21T04:18:01Z","timestamp":1776745081000},"page":"831-838","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Comparative Hemolytic Effects of Ex\u00a0Vivo Lung Perfusion Perfusates: An In\u00a0Vitro Blood Loop Approach"],"prefix":"10.1111","volume":"50","author":[{"given":"Chris H. H.","family":"Chan","sequence":"first","affiliation":[{"name":"Innovative Device &amp; Engineering Applications Lab, the Texas Heart Institute at Baylor College of Medicine  Houston Texas USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Asma F.","family":"Farooqui","sequence":"additional","affiliation":[{"name":"Innovative Device &amp; Engineering Applications Lab, the Texas Heart Institute at Baylor College of Medicine  Houston Texas USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christopher","family":"Tan","sequence":"additional","affiliation":[{"name":"Innovative Device &amp; Engineering Applications Lab, the Texas Heart Institute at Baylor College of Medicine  Houston Texas USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wilson","family":"Lim","sequence":"additional","affiliation":[{"name":"Innovative Device &amp; Engineering Applications Lab, the Texas Heart Institute at Baylor College of Medicine  Houston Texas USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brian","family":"Quach","sequence":"additional","affiliation":[{"name":"Innovative Device &amp; Engineering Applications Lab, the Texas Heart Institute at Baylor College of Medicine  Houston Texas USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gabriel","family":"Loor","sequence":"additional","affiliation":[{"name":"Division of Cardiopulmonary Transplantation and Mechanical Circulatory Support, Michael E DeBakey Department of Surgery Baylor College of Medicine  Houston Texas USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yaxin","family":"Wang","sequence":"additional","affiliation":[{"name":"Innovative Device &amp; Engineering Applications Lab, the Texas Heart Institute at Baylor College of Medicine  Houston Texas USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,4,20]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1097\/mot.0000000000001065"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/s2213\u20102600(18)30136\u2010x"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S2213\u20102600(19)30200\u20100"},{"key":"e_1_2_11_5_1","article-title":"Long\u2010Term Outcomes of the International EXPAND Trial of Organ Care System (OCS) Lung Preservation for Lung Transplantation","volume":"85","author":"Loor G. W. G.","year":"2025","journal-title":"Lancet"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467\u2010020\u201019581\u20104"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/ajt.15572"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.healun.2017.09.007"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467\u2010022\u201031811\u20105"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2020.01323"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1161\/circulationaha.110.008698"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1161\/atvbaha.123.318780"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1097\/TP.0000000000004503"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1172\/jci.insight.98546"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajplung.00155.2015"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.healun.2025.02.1188"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.freeradbiomed.2003.11.025"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.3390\/diagnostics14070680"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2025.1513546"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2024.1358153"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jtcvs.2017.08.036"},{"key":"e_1_2_11_22_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.cir.97.6.562"},{"key":"e_1_2_11_23_1","doi-asserted-by":"publisher","DOI":"10.1111\/trf.14800"},{"key":"e_1_2_11_24_1","doi-asserted-by":"publisher","DOI":"10.1097\/tp.0000000000001616"},{"key":"e_1_2_11_25_1","doi-asserted-by":"publisher","DOI":"10.1109\/EMBC53108.2024.10782017"},{"key":"e_1_2_11_26_1","doi-asserted-by":"publisher","DOI":"10.1051\/ject\/200335128"},{"key":"e_1_2_11_27_1","doi-asserted-by":"publisher","DOI":"10.3390\/membranes11050313"},{"key":"e_1_2_11_28_1","doi-asserted-by":"publisher","DOI":"10.1177\/1535370219834498"},{"key":"e_1_2_11_29_1","doi-asserted-by":"publisher","DOI":"10.1002\/bjs.5324"},{"key":"e_1_2_11_30_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41587\u2010022\u201001354\u20107"},{"key":"e_1_2_11_31_1","doi-asserted-by":"publisher","DOI":"10.1097\/mat.0000000000000550"},{"key":"e_1_2_11_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajt.2022.11.027"},{"key":"e_1_2_11_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.healun.2008.09.003"},{"key":"e_1_2_11_34_1","doi-asserted-by":"publisher","DOI":"10.1097\/mat.0000000000000173"},{"key":"e_1_2_11_35_1","doi-asserted-by":"publisher","DOI":"10.1111\/aor.13771"},{"key":"e_1_2_11_36_1","doi-asserted-by":"publisher","DOI":"10.1186\/s40635\u2010019\u20100264\u2010z"},{"key":"e_1_2_11_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbiomech.2021.110898"},{"key":"e_1_2_11_38_1","doi-asserted-by":"publisher","DOI":"10.1039\/d2tb00379a"},{"key":"e_1_2_11_39_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12288\u2010016\u20100769\u20105"},{"key":"e_1_2_11_40_1","doi-asserted-by":"publisher","DOI":"10.1097\/tp.0000000000003931"},{"key":"e_1_2_11_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.transproceed.2019.03.042"},{"key":"e_1_2_11_42_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2024.1365964"},{"key":"e_1_2_11_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.healun.2011.07.014"}],"container-title":["Artificial Organs"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/aor.70138","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/aor.70138","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/aor.70138","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,6,4]],"date-time":"2026-06-04T05:17:29Z","timestamp":1780550249000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/aor.70138"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,4,20]]},"references-count":42,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2026,6]]}},"alternative-id":["10.1111\/aor.70138"],"URL":"https:\/\/doi.org\/10.1111\/aor.70138","archive":["Portico"],"relation":{},"ISSN":["0160-564X","1525-1594"],"issn-type":[{"value":"0160-564X","type":"print"},{"value":"1525-1594","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,4,20]]},"assertion":[{"value":"2025-10-23","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-03-12","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-04-20","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}