{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,29]],"date-time":"2026-08-29T06:16:45Z","timestamp":1787984205542,"version":"build-2784847793"},"reference-count":60,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2026,4,26]],"date-time":"2026-04-26T00:00:00Z","timestamp":1777161600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,4,26]],"date-time":"2026-04-26T00:00:00Z","timestamp":1777161600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100002347","name":"Bundesministerium f\u00fcr Forschung, Technologie und Raumfahrt","doi-asserted-by":"publisher","award":["13GW0498"],"award-info":[{"award-number":["13GW0498"]}],"id":[{"id":"10.13039\/501100002347","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100018703","name":"HORIZON EUROPE European Innovation Council","doi-asserted-by":"publisher","award":["190188598"],"award-info":[{"award-number":["190188598"]}],"id":[{"id":"10.13039\/100018703","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Artificial Organs"],"published-print":{"date-parts":[[2026,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Introduction<\/jats:title>\n                    <jats:p>Valvular heart disease remains a leading cause of morbidity and mortality. Current biological and mechanical heart valve prostheses have significant limitations and fail to address the patient's needs. Most of the limitations arise from the use of foreign materials, which can largely be addressed by utilizing autologous pericardial tissue. However, not all patients have usable pericardium due to previous interventions. This study investigates peritoneum as a potential alternative to pericardium and evaluates an optimized treatment method for intraoperative tissue treatment.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>A previously established cross\u2010linking method was optimized and applied to pericardial and peritoneal tissues. The tissues were characterized with respect to biochemical composition, cross\u2010linking degree, biomechanical properties, and structural organization. Furthermore, the feasibility of valve shaping was assessed, and constructs were subjected to acute hydrodynamic testing.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Tissue treated with the optimized cross\u2010linking method showed high cytocompatibility and was successfully applied to peritoneal tissue. Compared to pericardium, peritoneum exhibited a higher elastin content and a looser structural organization, resulting in distinct mechanical behavior. Both tissues could be shaped into valve constructs showing adequate acute hydrodynamic behavior under the applied test conditions.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>The findings suggest that the peritoneum is a promising candidate material for further investigation as a heart valve scaffold. However, differences in microstructure and mechanical behavior highlight the need for further testing, particularly regarding long\u2010term durability, cyclic loading behavior, and tissue remodeling capacity.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/aor.70151","type":"journal-article","created":{"date-parts":[[2026,4,27]],"date-time":"2026-04-27T03:41:45Z","timestamp":1777261305000},"page":"1237-1253","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Identification of Autologous Tissue Sources and Optimization of a Treatment Method for Intraoperative Manufacturing of Heart Valve Constructs"],"prefix":"10.1111","volume":"50","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3337-9083","authenticated-orcid":false,"given":"Marvin","family":"Steitz","sequence":"first","affiliation":[{"name":"Department of Congenital Heart Disease\u2014Pediatric Cardiology Deutsches Herzzentrum der Charit\u00e9  Berlin Germany"},{"name":"Charit\u00e9\u2014Universit\u00e4tsmedizin Berlin, Corporate Member of Freie Universit\u00e4t Berlin Humboldt\u2010Universit\u00e4t zu Berlin  Berlin Germany"},{"name":"DZHK (German Centre for Cardiovascular Research) Partner Site Berlin  Berlin Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5642-2219","authenticated-orcid":false,"given":"Mahamuda Badhon","family":"Khan","sequence":"additional","affiliation":[{"name":"Charit\u00e9\u2014Universit\u00e4tsmedizin Berlin, Corporate Member of Freie Universit\u00e4t Berlin Humboldt\u2010Universit\u00e4t zu Berlin  Berlin Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6202-9859","authenticated-orcid":false,"given":"Katharina","family":"Fritsch","sequence":"additional","affiliation":[{"name":"Department Dynamics and Transport in Quantum Materials Helmholtz\u2010Zentrum Berlin f\u00fcr Materialien und Energie GmbH  Berlin Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7006-2897","authenticated-orcid":false,"given":"Alexander","family":"Breitenstein\u2010Attach","sequence":"additional","affiliation":[{"name":"Department of Congenital Heart Disease\u2014Pediatric Cardiology Deutsches Herzzentrum der Charit\u00e9  Berlin Germany"},{"name":"Charit\u00e9\u2014Universit\u00e4tsmedizin Berlin, Corporate Member of Freie Universit\u00e4t Berlin Humboldt\u2010Universit\u00e4t zu Berlin  Berlin Germany"},{"name":"DZHK (German Centre for Cardiovascular Research) Partner Site Berlin  Berlin Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jana Katharina Betty","family":"Korbacher","sequence":"additional","affiliation":[{"name":"Charit\u00e9\u2014Universit\u00e4tsmedizin Berlin, Corporate Member of Freie Universit\u00e4t Berlin Humboldt\u2010Universit\u00e4t zu Berlin  Berlin Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jordi Joaquin","family":"Modolell Y Mainou","sequence":"additional","affiliation":[{"name":"Department of Congenital Heart Disease\u2014Pediatric Cardiology Deutsches Herzzentrum der Charit\u00e9  Berlin Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katrin","family":"Schrimpf","sequence":"additional","affiliation":[{"name":"Charit\u00e9\u2014Universit\u00e4tsmedizin Berlin, Corporate Member of Freie Universit\u00e4t Berlin Humboldt\u2010Universit\u00e4t zu Berlin  Berlin Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8556-116X","authenticated-orcid":false,"given":"Robert","family":"Ramm","sequence":"additional","affiliation":[{"name":"Lower Saxony Centre for Biomedical Engineering, Implant Research and Development University of Veterinary Medicine Hanover  Hannover Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7571-3775","authenticated-orcid":false,"given":"Sara","family":"Knigge","sequence":"additional","affiliation":[{"name":"Lower Saxony Centre for Biomedical Engineering, Implant Research and Development University of Veterinary Medicine Hanover  Hannover Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7294-5140","authenticated-orcid":false,"given":"Katja","family":"Eildermann","sequence":"additional","affiliation":[{"name":"Department of Pediatric Cardiology and Intensive Care Medicine Georg\u2010August University G\u00f6ttingen  G\u00f6ttingen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4216-4663","authenticated-orcid":false,"given":"Georg","family":"Arlt","sequence":"additional","affiliation":[{"name":"Department of Congenital Heart Disease\u2014Pediatric Cardiology Deutsches Herzzentrum der Charit\u00e9  Berlin Germany"},{"name":"Department of General Surgery, Minimally Invasive and Visceral Surgery, Selected Vascular Surgery Park\u2010Klinik Wei\u00dfensee  Berlin Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9155-9509","authenticated-orcid":false,"given":"Antonia","family":"Schulz","sequence":"additional","affiliation":[{"name":"Charit\u00e9\u2014Universit\u00e4tsmedizin Berlin, Corporate Member of Freie Universit\u00e4t Berlin Humboldt\u2010Universit\u00e4t zu Berlin  Berlin Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-0941-1199","authenticated-orcid":false,"given":"Jonathan Frederik Sebastian","family":"Kiekenap","sequence":"additional","affiliation":[{"name":"Department of Congenital Heart Disease\u2014Pediatric Cardiology Deutsches Herzzentrum der Charit\u00e9  Berlin Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Boris","family":"Warnack","sequence":"additional","affiliation":[{"name":"Warnack Medconsult Untere Wenkenhofstrasse  Riehen Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4780-5271","authenticated-orcid":false,"given":"Peter","family":"Kramer","sequence":"additional","affiliation":[{"name":"Charit\u00e9\u2014Universit\u00e4tsmedizin Berlin, Corporate Member of Freie Universit\u00e4t Berlin Humboldt\u2010Universit\u00e4t zu Berlin  Berlin Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4401-5936","authenticated-orcid":false,"given":"Frank","family":"Edelmann","sequence":"additional","affiliation":[{"name":"Charit\u00e9\u2014Universit\u00e4tsmedizin Berlin, Corporate Member of Freie Universit\u00e4t Berlin Humboldt\u2010Universit\u00e4t zu Berlin  Berlin Germany"},{"name":"DZHK (German Centre for Cardiovascular Research) Partner Site Berlin  Berlin Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7881-1557","authenticated-orcid":false,"given":"Felix","family":"Berger","sequence":"additional","affiliation":[{"name":"Department of Congenital Heart Disease\u2014Pediatric Cardiology Deutsches Herzzentrum der Charit\u00e9  Berlin Germany"},{"name":"Charit\u00e9\u2014Universit\u00e4tsmedizin Berlin, Corporate Member of Freie Universit\u00e4t Berlin Humboldt\u2010Universit\u00e4t zu Berlin  Berlin Germany"},{"name":"DZHK (German Centre for Cardiovascular Research) Partner Site Berlin  Berlin Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6987-1264","authenticated-orcid":false,"given":"Boris","family":"Schmitt","sequence":"additional","affiliation":[{"name":"Department of Congenital Heart Disease\u2014Pediatric Cardiology Deutsches Herzzentrum der Charit\u00e9  Berlin Germany"},{"name":"Charit\u00e9\u2014Universit\u00e4tsmedizin Berlin, Corporate Member of Freie Universit\u00e4t Berlin Humboldt\u2010Universit\u00e4t zu Berlin  Berlin Germany"},{"name":"DZHK (German Centre for Cardiovascular Research) Partner Site Berlin  Berlin Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,4,26]]},"reference":[{"key":"e_1_2_13_2_1","first-page":"158","volume-title":"Blutzirkulation","author":"Moritz F.","year":"1926"},{"key":"e_1_2_13_3_1","unstructured":"R.Zeeman \u201cCross\u2010Linking of Collagen\u2010Based Materials \u201d (1998)."},{"key":"e_1_2_13_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41569-020-0422-8"},{"key":"e_1_2_13_5_1","doi-asserted-by":"publisher","DOI":"10.1161\/JAHA.117.006339"},{"key":"e_1_2_13_6_1","doi-asserted-by":"publisher","DOI":"10.5830\/CVJA-2013-028"},{"key":"e_1_2_13_7_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcvm.2023.1137827"},{"key":"e_1_2_13_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mtbio.2019.100038"},{"key":"e_1_2_13_9_1","doi-asserted-by":"publisher","DOI":"10.1161\/JAHA.120.018506"},{"key":"e_1_2_13_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10856-020-06462-x"},{"key":"e_1_2_13_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-4975(89)90623-1"},{"key":"e_1_2_13_12_1","doi-asserted-by":"publisher","DOI":"10.1080\/09205063.2018.1429732"},{"key":"e_1_2_13_13_1","doi-asserted-by":"publisher","DOI":"10.4414\/smw.2018.14612"},{"key":"e_1_2_13_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2006.02.008"},{"key":"e_1_2_13_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(62)90345-8"},{"key":"e_1_2_13_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jacc.2016.04.023"},{"key":"e_1_2_13_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jtcvs.2012.11.012"},{"key":"e_1_2_13_18_1","unstructured":"\u00c4rztezeitung \u201cAortenklappe aus Eigenem Herzgewebe [Internet] \u201d zitiert Januar 3 2025 https:\/\/www.aerztezeitung.de\/Medizin\/Aortenklappe\u2010aus\u2010eigenem\u2010Herzgewebe\u2010411303.html."},{"key":"e_1_2_13_19_1","doi-asserted-by":"publisher","DOI":"10.1093\/ejcts\/ezaa345"},{"key":"e_1_2_13_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-5223(19)43054-7"},{"key":"e_1_2_13_21_1","doi-asserted-by":"publisher","DOI":"10.3109\/14017437509139182"},{"key":"e_1_2_13_22_1","doi-asserted-by":"publisher","DOI":"10.1002\/ccd.30992"},{"key":"e_1_2_13_23_1","doi-asserted-by":"publisher","DOI":"10.3390\/bioengineering10111247"},{"key":"e_1_2_13_24_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-4636(199802)39:2<207::AID-JBM6>3.0.CO;2-T"},{"key":"e_1_2_13_25_1","doi-asserted-by":"publisher","DOI":"10.1042\/BA20090101"},{"key":"e_1_2_13_26_1","doi-asserted-by":"publisher","DOI":"10.1098\/rstb.2007.2126"},{"key":"e_1_2_13_27_1","doi-asserted-by":"publisher","DOI":"10.1089\/ten.2006.0279"},{"key":"e_1_2_13_28_1","unstructured":"DIN \u201cDIN EN ISO 10993\u2010Biologische Beurteilung von Medizinprodukten\u2014Teil 5: Pr\u00fcfungen auf In\u2010Vitro\u2010Zytotoxizit\u00e4t \u201d (2009)."},{"key":"e_1_2_13_29_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10561-009-9155-y"},{"key":"e_1_2_13_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2011.09.027"},{"key":"e_1_2_13_31_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsbiomaterials.8b01311"},{"key":"e_1_2_13_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/0142-9612(95)92125-P"},{"key":"e_1_2_13_33_1","doi-asserted-by":"publisher","DOI":"10.3390\/biom10030371"},{"issue":"4","key":"e_1_2_13_34_1","first-page":"463","article-title":"Tissue Engineering of Cardiac Valve Prostheses II: Biomechanical Characterization of Decellularized Porcine Aortic Heart Valves","volume":"11","author":"Korossis S. A.","year":"2002","journal-title":"Journal of Heart Valve Disease"},{"key":"e_1_2_13_35_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-018-31388-4"},{"key":"e_1_2_13_36_1","doi-asserted-by":"publisher","DOI":"10.1093\/ejcts\/ezae027"},{"key":"e_1_2_13_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.athoracsur.2019.03.020"},{"key":"e_1_2_13_38_1","unstructured":"DIN \u201cDIN EN ISO 5840\u2010Herz\u2010und Gef\u00e4\u00dfimplantate\u2010Herzklappenprothesen \u201d (2019)."},{"key":"e_1_2_13_39_1","volume-title":"Handbook of Material Behavior","author":"Holzapfel G. A.","year":"2000"},{"key":"e_1_2_13_40_1","doi-asserted-by":"publisher","DOI":"10.1089\/ten.tec.2009.0309"},{"key":"e_1_2_13_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.carpath.2022.107457"},{"key":"e_1_2_13_42_1","doi-asserted-by":"publisher","DOI":"10.1093\/ejcts\/ezt623"},{"key":"e_1_2_13_43_1","doi-asserted-by":"publisher","DOI":"10.4265\/bio.18.221"},{"key":"e_1_2_13_44_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCULATIONAHA.105.549311"},{"key":"e_1_2_13_45_1","doi-asserted-by":"publisher","DOI":"10.1089\/ten.tec.2022.0028"},{"key":"e_1_2_13_46_1","volume-title":"Biochemistry, Glycosaminoglycans","author":"Casale J.","year":"2023"},{"key":"e_1_2_13_47_1","doi-asserted-by":"publisher","DOI":"10.1002\/path.1400"},{"key":"e_1_2_13_48_1","doi-asserted-by":"publisher","DOI":"10.1089\/ten.teb.2014.0290"},{"key":"e_1_2_13_49_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13287-020-02021-x"},{"key":"e_1_2_13_50_1","doi-asserted-by":"publisher","DOI":"10.1155\/2014\/189197"},{"key":"e_1_2_13_51_1","doi-asserted-by":"publisher","DOI":"10.1177\/0885328213479047"},{"key":"e_1_2_13_52_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-323-22805-3.00012-8"},{"key":"e_1_2_13_53_1","doi-asserted-by":"publisher","DOI":"10.5761\/atcs.nm.20-00125"},{"key":"e_1_2_13_54_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1369-7021(11)70059-1"},{"key":"e_1_2_13_55_1","unstructured":"Kenhub \u201cPericardium: Anatomy of Fibrous and Serous Layers | Kenhub [Internet] \u201d zitiert Januar 3 2025 https:\/\/www.kenhub.com\/en\/library\/anatomy\/the\u2010pericardium."},{"key":"e_1_2_13_56_1","doi-asserted-by":"publisher","DOI":"10.1159\/000377624"},{"issue":"4","key":"e_1_2_13_57_1","doi-asserted-by":"crossref","DOI":"10.5339\/qproc.2012.heartvalve.4.38","article-title":"The Cellular and Extracellular Matrix Structure of Human Pericardium for Heart Valve Tissue Engineering","volume":"2012","author":"Schornik D.","year":"2012","journal-title":"QScience Proceedings"},{"key":"e_1_2_13_58_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejphar.2020.173090"},{"key":"e_1_2_13_59_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0142-9612(97)00215-9"},{"key":"e_1_2_13_60_1","first-page":"586","volume-title":"Clinical Anatomy of the Spine, Spinal Cord, and ANS","author":"Cramer G. D.","year":"2013"},{"key":"e_1_2_13_61_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.prp.2019.03.024"}],"container-title":["Artificial Organs"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/aor.70151","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/aor.70151","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/aor.70151","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,29]],"date-time":"2026-08-29T05:28:11Z","timestamp":1787981291000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/aor.70151"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,4,26]]},"references-count":60,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2026,9]]}},"alternative-id":["10.1111\/aor.70151"],"URL":"https:\/\/doi.org\/10.1111\/aor.70151","archive":["Portico"],"relation":{},"ISSN":["0160-564X","1525-1594"],"issn-type":[{"value":"0160-564X","type":"print"},{"value":"1525-1594","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,4,26]]},"assertion":[{"value":"2026-02-10","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-04-13","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-04-26","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}