{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T17:55:19Z","timestamp":1790963719516,"version":"4.1.0"},"reference-count":30,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T00:00:00Z","timestamp":1790726400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T00:00:00Z","timestamp":1790726400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Artificial Organs"],"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>The immunomodulatory activity of normothermic machine perfusion (NMP) on donor organs may be reflected by the release of immune\u2010regulating factors into the perfusate. Dendritic cells (DCs) are potent antigen\u2010presenting cells and are able to sense these factors. We hypothesized that activation of DCs could be used to monitor the release of these mediators into the perfusate.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Human monocytes from buffy coats were differentiated into monocyte\u2010derived DCs (moDCs). Perfusate was obtained from human donor kidneys deemed unsuitable for transplantation (\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u200915) that underwent 6\u2009h of NMP. MoDCs were incubated for 24\u2009h with perfusate collected at different timepoints. DC supernatant was analyzed with Luminex and ELISA. MoDCs were analyzed with flow cytometry for maturation markers and co\u2010cultured with allogenic T cells.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Incubation with perfusate resulted in moDCs with a reduced surface expression of CD86, without affecting other maturation markers. Analysis of DC supernatant showed there was a specific increase of IL\u201010 production upon incubation with perfusate. As a consequence, moDCs incubated with perfusate showed lower T cell stimulatory capacity.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>This study showed that the activation status of DCs can be used to monitor the release of factors into the perfusate during NMP with an anti\u2010inflammatory DC phenotype and function. It demonstrates the feasibility of a cell\u2010based model to determine immune regulatory effects during ex\u00a0vivo organ perfusion.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/aor.70251","type":"journal-article","created":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T06:31:38Z","timestamp":1790836298000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["The Anti\u2010Inflammatory Effect of Normothermic Machine Perfused Human Kidneys on Monocyte\u2010Derived Dendritic Cells"],"prefix":"10.1111","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7923-5379","authenticated-orcid":false,"given":"Laura W. D.","family":"Knijff","sequence":"first","affiliation":[{"name":"Department of Nephrology Leiden University Medical Center  Leiden the Netherlands"},{"name":"Transplant Center Leiden University Medical Center  Leiden the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2918-171X","authenticated-orcid":false,"given":"Asel S.","family":"Arykbaeva","sequence":"additional","affiliation":[{"name":"Transplant Center Leiden University Medical Center  Leiden the Netherlands"},{"name":"Department of Surgery Leiden University Medical Center  Leiden the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-2925-6429","authenticated-orcid":false,"given":"Sandra W.","family":"van der Kooij","sequence":"additional","affiliation":[{"name":"Department of Nephrology Leiden University Medical Center  Leiden the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2206-3929","authenticated-orcid":false,"given":"Dorottya K.","family":"de Vries","sequence":"additional","affiliation":[{"name":"Transplant Center Leiden University Medical Center  Leiden the Netherlands"},{"name":"Department of Surgery Leiden University Medical Center  Leiden the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1370-2963","authenticated-orcid":false,"given":"Mieke F.","family":"van Essen","sequence":"additional","affiliation":[{"name":"Department of Nephrology Leiden University Medical Center  Leiden the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0603-9849","authenticated-orcid":false,"given":"Ian P. J.","family":"Alwayn","sequence":"additional","affiliation":[{"name":"Transplant Center Leiden University Medical Center  Leiden the Netherlands"},{"name":"Department of Surgery Leiden University Medical Center  Leiden the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5036-2999","authenticated-orcid":false,"given":"Henri G. D.","family":"Leuvenink","sequence":"additional","affiliation":[{"name":"Department of Surgery University Medical Center Groningen  Groningen the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7801-665X","authenticated-orcid":false,"given":"Rutger J.","family":"Ploeg","sequence":"additional","affiliation":[{"name":"Transplant Center Leiden University Medical Center  Leiden the Netherlands"},{"name":"Nuffield Department of Surgical Sciences Oxford University  Oxford UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6257-0899","authenticated-orcid":false,"given":"Cees","family":"van Kooten","sequence":"additional","affiliation":[{"name":"Department of Nephrology Leiden University Medical Center  Leiden the Netherlands"},{"name":"Transplant Center Leiden University Medical Center  Leiden the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,9,30]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/S41591\u2010023\u201002376\u20107"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.29337\/IJSP.165"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/AJT.12179"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1097\/TP.0000000000004098"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467\u2010025\u201059829\u20105"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/AJT.14294"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.3389\/FIMMU.2022.940094\/BIBTEX"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1152\/AJPGI.00266.2021\/ASSET\/IMAGES\/LARGE\/AJPGI.00266.2021_F005.JPEG"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/LT.25429"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/AJT.16334"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20052203"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.3389\/FIMMU.2021.654540\/BIBTEX"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.4049\/JIMMUNOL.162.6.3256"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1371\/JOURNAL.PONE.0045185"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/JLB.3MR0621\u2010616RR"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/J.1523\u20101755.2005.00502.X"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/J.JIM.2020.112748"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.4049\/JIMMUNOL.164.5.2405"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1159\/000334245"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.3791\/54968"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1111\/AJT.16371"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.3389\/FIMMU.2022.831371\/FULL"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1111\/AOR.70080"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1097\/TP.0000000000004215"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.7150\/THNO.21072"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1371\/JOURNAL.PONE.0202550"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.33594\/000000102"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1097\/TXD.0000000000001503"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/J.CELREP.2015.01.013"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1111\/TRI.13504"}],"container-title":["Artificial Organs"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/aor.70251","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/aor.70251","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/aor.70251","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T17:39:58Z","timestamp":1790962798000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/aor.70251"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9,30]]},"references-count":30,"alternative-id":["10.1111\/aor.70251"],"URL":"https:\/\/doi.org\/10.1111\/aor.70251","archive":["Portico"],"relation":{},"ISSN":["0160-564X","1525-1594"],"issn-type":[{"value":"0160-564X","type":"print"},{"value":"1525-1594","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9,30]]},"assertion":[{"value":"2025-10-08","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-09-11","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-09-30","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"aor.70251"}}