{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T13:14:04Z","timestamp":1782911644599,"version":"3.54.5"},"reference-count":44,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2014,2,16]],"date-time":"2014-02-16T00:00:00Z","timestamp":1392508800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Acta Ophthalmologica"],"published-print":{"date-parts":[[2014,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Purpose<\/jats:title><jats:p>DNA methylation is an epigenetic mark involved in the control of genes expression. Abnormal epigenetic events have been reported in human pathologies but weakly documented in eye diseases. The purpose of this study was to establish DNMT <jats:styled-content style=\"fixed-case\">mRNA<\/jats:styled-content> and protein expression levels in the anterior eye segment tissues and their related (primary or immortalized) cell cultures as a first step towards future <jats:italic>in vivo<\/jats:italic> and <jats:italic>in vitro<\/jats:italic> methylomic studies.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Total m<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> was extracted from human cornea, conjunctiva, anterior lens capsule, trabeculum and related cell cultures (cornea epithelial, trabecular meshwork, keratocytes for primary cells; and HCE, Chang, B\u20103 for immortalized cells). <jats:styled-content style=\"fixed-case\">cDNA<\/jats:styled-content> was quantified by real\u2010time PCR using specific primers for DNMT1, 2, 3A, 3B and 3L. Immunolocalization assays were carried out on human cornea using specific primary antibodies for DNMT1, 2 and 3A, 3B and 3L.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>All <jats:styled-content style=\"fixed-case\">DNMT<\/jats:styled-content> transcripts were detected in human cornea, conjunctiva, anterior lens capsule, trabeculum and related cells but showed statistically different expression patterns between tissues and cells. <jats:styled-content style=\"fixed-case\">DNMT<\/jats:styled-content>2 protein presented a specific and singular expression pattern in corneal endothelium.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>This study produced the first inventory of the expression patterns of <jats:styled-content style=\"fixed-case\">DNMT<\/jats:styled-content>s in human adult anterior eye segment. Our research highlights that <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> methylation cannot be ruled out as a way to bring new insights into well\u2010known ocular diseases. In addition, future <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> methylation studies using various cells as experimental models need to be conducted with attention to approach the results analysis from a global tissue perspective.<\/jats:p><\/jats:sec>","DOI":"10.1111\/aos.12365","type":"journal-article","created":{"date-parts":[[2014,2,17]],"date-time":"2014-02-17T11:44:46Z","timestamp":1392637486000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["<scp>DNA<\/scp> methyl transferases are differentially expressed in the human anterior eye segment"],"prefix":"10.1111","volume":"92","author":[{"given":"Nicolas","family":"Bonnin","sequence":"first","affiliation":[{"name":"EA 7281 R2D2 Biochemistry Laboratory Medicine School Auvergne University F\u201063000 Clermont\u2010Ferrand France"},{"name":"Internal Medicine\u2010Ophthalmology\u2010ENT Department Ophthalmology Clermont\u2010Ferrand 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