{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,25]],"date-time":"2026-08-25T18:45:49Z","timestamp":1787683549232,"version":"build-2784847793"},"reference-count":15,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2014,3,16]],"date-time":"2014-03-16T00:00:00Z","timestamp":1394928000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Acta Ophthalmologica"],"published-print":{"date-parts":[[2014,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Purpose<\/jats:title><jats:p>To overcome problems and delays of the preparation of autologous serum eye drops, a production line of <jats:styled-content style=\"fixed-case\">ABO<\/jats:styled-content>\u2010specific allogeneic serum eye drops from male blood donors was set up in a blood bank. Feasibility, clinical routine, safety and efficacy were evaluated in a cohort of patients with severe ocular surface disorders.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Serum was derived from 450\u00a0ml whole\u2010blood donations from regular male blood donors, produced and tested according to good manufacturing practice and legislation regulating blood products in Denmark. Serum was diluted to 20% (v\/v) with NaCl 0.9%, filtered, bottled, registered and stored at \u221230\u00b0C in the blood bank. Upon request, frozen <jats:styled-content style=\"fixed-case\">ABO<\/jats:styled-content>\u2010identical serum drops in lots of 14 bottles could be provided immediately. Safety and efficacy were evaluated in 34 patients with severe ocular surface disease refractory to conventional medical therapy. Patients were treated six times daily for minimum 2\u20134\u00a0weeks. Objective findings and subjective symptoms were compared between day 0 and after 4\u00a0weeks of treatment using the <jats:styled-content style=\"fixed-case\">W<\/jats:styled-content>ilcoxon signed\u2010rank test.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Clinically, no side\u2010effects were observed. In total, 59% of the patients with ocular surface changes improved objectively (slit\u2010lamp examination). Partial or full healing of corneal changes, as well as subjective relief of symptoms, was observed in 16 of 20 patients with keratoconjunctivitis sicca (p\u00a0&lt;\u00a00.001). The 14 patients with persistent epithelial defect experienced neither objective nor subjective improvements during serum treatment.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Ready\u2010made <jats:styled-content style=\"fixed-case\">ABO<\/jats:styled-content>\u2010identical allogeneic serum eye drops were straightforwardly produced, quality\u2010assured and registered as a safe standard blood product for the treatment of certain cases of severe dry eye disease. Therapeutic efficacy was comparable to previous reports on autologous serum drops.<\/jats:p><\/jats:sec>","DOI":"10.1111\/aos.12386","type":"journal-article","created":{"date-parts":[[2014,3,17]],"date-time":"2014-03-17T02:40:45Z","timestamp":1395024045000},"page":"783-786","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":53,"title":["Ready\u2010made allogeneic <scp>ABO<\/scp>\u2010specific serum eye drops: production from regular male blood donors, clinical routine, safety and efficacy"],"prefix":"10.1111","volume":"92","author":[{"given":"Lene Holm","family":"Harritsh\u00f8j","sequence":"first","affiliation":[{"name":"Department of Clinical Immunology Blood Bank Rigshospitalet Copenhagen Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Connie","family":"Nielsen","sequence":"additional","affiliation":[{"name":"Department of Clinical Immunology Blood Bank Rigshospitalet Copenhagen Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Henrik","family":"Ullum","sequence":"additional","affiliation":[{"name":"Department of Clinical Immunology Blood Bank Rigshospitalet Copenhagen Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Morten Bagge","family":"Hansen","sequence":"additional","affiliation":[{"name":"Department of Clinical Immunology Blood Bank Rigshospitalet Copenhagen Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hanne Olsen","family":"Julian","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology Glostrup Hospital Copenhagen Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2014,3,16]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1136\/bjophthalmol-2012-301668"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.eye.6703079"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/art.1780270415"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1136\/bjo.2004.044347"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/aos.12167"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1089\/jop.2012.0002"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1136\/bjo.2003.026211"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bmt.1703862"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1755-3768.2012.02521.x"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1136\/bjo.85.10.1188"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1097\/00003226-200111000-00005"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9394(14)71700-3"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1097\/00004397-200010000-00009"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajo.2007.03.016"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1755-3768.2012.02538.x"}],"container-title":["Acta Ophthalmologica"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Faos.12386","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/aos.12386","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,23]],"date-time":"2023-09-23T20:08:13Z","timestamp":1695499693000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/aos.12386"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,3,16]]},"references-count":15,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2014,12]]}},"alternative-id":["10.1111\/aos.12386"],"URL":"https:\/\/doi.org\/10.1111\/aos.12386","archive":["Portico"],"relation":{},"ISSN":["1755-375X","1755-3768"],"issn-type":[{"value":"1755-375X","type":"print"},{"value":"1755-3768","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,3,16]]},"assertion":[{"value":"2013-11-01","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2014-02-08","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2014-03-16","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}