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In the <jats:styled-content style=\"fixed-case\">LSCD<\/jats:styled-content> model, one group received <jats:styled-content style=\"fixed-case\">CV<\/jats:styled-content> containing human limbal epithelial cells (<jats:styled-content style=\"fixed-case\">CV<\/jats:styled-content>\u00a0+\u00a0<jats:styled-content style=\"fixed-case\">hLEC<\/jats:styled-content> group, <jats:italic>n<\/jats:italic>\u00a0=\u00a02) and the other was an untreated control (<jats:italic>n<\/jats:italic>\u00a0=\u00a01). Gross observation, including fluorescent staining, pathological examination, immunohistochemistry and electron microscopy, was used to evaluate the effect of <jats:styled-content style=\"fixed-case\">CV<\/jats:styled-content> on the corneal epithelium.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>In the stromal wound model, fluorescent staining showed that epithelial reconstruction occurred as rapidly in the <jats:styled-content style=\"fixed-case\">CV<\/jats:styled-content>\u00a0+\u00a0<jats:styled-content style=\"fixed-case\">FG<\/jats:styled-content> group as it did in the control group. The pathological examination proved that the <jats:styled-content style=\"fixed-case\">CV<\/jats:styled-content> supported a healthy corneal epithelium in the <jats:styled-content style=\"fixed-case\">CV<\/jats:styled-content>\u00a0+\u00a0<jats:styled-content style=\"fixed-case\">FG<\/jats:styled-content> group, whereas <jats:styled-content style=\"fixed-case\">FG<\/jats:styled-content> led to hypertrophy and inappropriate differentiation of corneal epithelium in the <jats:styled-content style=\"fixed-case\">FG<\/jats:styled-content> group. In the <jats:styled-content style=\"fixed-case\">LSCD<\/jats:styled-content> model, the corneas in the <jats:styled-content style=\"fixed-case\">CV<\/jats:styled-content>\u00a0+\u00a0h<jats:styled-content style=\"fixed-case\">LEC<\/jats:styled-content> group showed sustained tissue transparency with good epithelialization, low inflammatory response and reduced neovascularization. However, the control cornea was translucent and showed high amounts of inflammation and neovascularization.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>We have demonstrated that <jats:styled-content style=\"fixed-case\">CV<\/jats:styled-content> supports corneal epithelial differentiation and prevents epithelial hypertrophy, in addition to serving as a scaffold for h<jats:styled-content style=\"fixed-case\">LEC<\/jats:styled-content> transplantation, without complications.<\/jats:p><\/jats:sec>","DOI":"10.1111\/aos.12503","type":"journal-article","created":{"date-parts":[[2014,12,11]],"date-time":"2014-12-11T11:06:37Z","timestamp":1418295997000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":47,"title":["Regeneration of corneal epithelium utilizing a collagen vitrigel membrane in rabbit models for corneal stromal wound and limbal stem cell deficiency"],"prefix":"10.1111","volume":"93","author":[{"given":"J. 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