{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,17]],"date-time":"2025-09-17T16:13:03Z","timestamp":1758125583095,"version":"3.37.3"},"reference-count":35,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2015,1,28]],"date-time":"2015-01-28T00:00:00Z","timestamp":1422403200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Slovenian Research Agency (ARRS)","award":["P3-0333"],"award-info":[{"award-number":["P3-0333"]}]},{"name":"T\u00c1MOP","award":["4.2.1.\/B-09\/1\/KONV-2010-0007"],"award-info":[{"award-number":["4.2.1.\/B-09\/1\/KONV-2010-0007"]}]},{"DOI":"10.13039\/501100004895","name":"European Social Fund","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100004895","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Acta Ophthalmologica"],"published-print":{"date-parts":[[2015,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Purpose<\/jats:title><jats:p>To determine the structural characteristics of lens epithelial cells (<jats:styled-content style=\"fixed-case\">LEC<\/jats:styled-content>s) found on the anterior portion of the lens capsule and their pluripotency, proliferating and migrating potential when grown <jats:italic>ex vivo<\/jats:italic> with relevance to posterior capsular opacification (<jats:styled-content style=\"fixed-case\">PCO<\/jats:styled-content>) after cataract surgery.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>The explants of anterior portion of the lens capsule consisting of monolayer of <jats:styled-content style=\"fixed-case\">LEC<\/jats:styled-content>s were obtained from uneventful cataract surgery and were cultivated under adherent conditions. The size and shape of the outgrowing cells were recorded by scanning electron microscopy (<jats:styled-content style=\"fixed-case\">SEM<\/jats:styled-content>), while their migration and proliferation potential were followed using light microscopy. Positivity for proliferation (Ki\u201067)\u2010 and pluripotency (Sox2)\u2010specific markers were tested by immunofluorescent staining.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The proliferation and migration of anterior portion of the lens capsule's LECs filling up the denuded and reverse side regions of the lens capsule as well as their growth on glass culture surfaces could be followed by light microscopy and <jats:styled-content style=\"fixed-case\">SEM<\/jats:styled-content>, while the distribution of <jats:styled-content style=\"fixed-case\">LEC<\/jats:styled-content>s and their morphology could be analysed in detail by <jats:styled-content style=\"fixed-case\">SEM<\/jats:styled-content>. The expression of Ki\u201067 and Sox2 in <jats:styled-content style=\"fixed-case\">LEC<\/jats:styled-content>s growing adherently on human anterior portion of the lens capsule could also be detected.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Classic light microscopy and <jats:styled-content style=\"fixed-case\">SEM<\/jats:styled-content> can be used to show that human anterior portion of the lens capsule harbours <jats:styled-content style=\"fixed-case\">LEC<\/jats:styled-content>s that can proliferate and migrate, suggesting their pluripotency or putative stem cell nature. Similarly, morphological techniques can be used to study <jats:styled-content style=\"fixed-case\">PCO<\/jats:styled-content> and the effect different drugs or physical treatments have against <jats:styled-content style=\"fixed-case\">PCO<\/jats:styled-content> development.<\/jats:p><\/jats:sec>","DOI":"10.1111\/aos.12655","type":"journal-article","created":{"date-parts":[[2015,1,29]],"date-time":"2015-01-29T01:15:51Z","timestamp":1422494151000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Morphological and proliferative studies on <i>ex vivo<\/i> cultured human anterior lens epithelial cells \u2013 relevance to capsular opacification"],"prefix":"10.1111","volume":"93","author":[{"given":"Sofija","family":"Andjeli\u0107","sequence":"first","affiliation":[{"name":"Eye Hospital University Medical Centre Ljubljana 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