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Included were patients with corneal ulcers that were non\u2010responsive to ointment or contact lenses and had been treated by amniotic membrane transplantation with either the overlay or sandwich procedure. Analysis parameters were visual acuity before and following treatment, recurrence rate and subjective comfort at the last follow\u2010up.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Of the 371 amniotic membrane transplantations that were conducted, 135 surgical treatments in 108 patients (51.9% male, 48.1% female; mean age 63.7\u00a0years) met the inclusion criteria. In total, 99 overlay and 36 multilayer amniotic membrane transplantations were performed. The follow\u2010up period was 47.5\u00a0\u00b1\u00a066.7\u00a0weeks (mean\u00a0\u00b1\u00a0<jats:styled-content style=\"fixed-case\">SD<\/jats:styled-content>). The recurrence rate at the last follow\u2010up was 47.8% with overlay membranes and 51.8% with the sandwich technique. There was no significant change in best\u2010corrected visual acuity following treatment with overlays (p<jats:italic>\u00a0<\/jats:italic>=<jats:italic>\u00a0<\/jats:italic>0.219) or sandwich procedure (p<jats:italic>\u00a0<\/jats:italic>=<jats:italic>\u00a0<\/jats:italic>0.703). At the last follow\u2010up, 72.1% (overlay) and 78.3% (sandwich) of the patients reported either no pain or increased comfort.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The recurrence rates and changes in visual acuity following overlay or sandwich amniotic membrane transplantation in patients suffering from corneal ulcer were lower than reported elsewhere in the literature. More than half of the patients profited from each of the amniotic membrane transplantation techniques with respect to recurrence and postoperative comfort.<\/jats:p><\/jats:sec>","DOI":"10.1111\/aos.12671","type":"journal-article","created":{"date-parts":[[2015,3,16]],"date-time":"2015-03-16T03:24:56Z","timestamp":1426476296000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Long\u2010term efficacy of glycerine\u2010processed amniotic membrane transplantation in patients with corneal ulcer"],"prefix":"10.1111","volume":"93","author":[{"given":"Constantin E.","family":"Uhlig","sequence":"first","affiliation":[{"name":"Department of Ophthalmology University Medical Center Muenster Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Charlotte","family":"Frings","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology University Medical Center Muenster 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