{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,11]],"date-time":"2026-04-11T01:37:48Z","timestamp":1775871468600,"version":"3.50.1"},"reference-count":17,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2015,7,1]],"date-time":"2015-07-01T00:00:00Z","timestamp":1435708800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100004853","name":"Chinese University of Hong Kong","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004853","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Acta Ophthalmologica"],"published-print":{"date-parts":[[2016,5]]},"abstract":"Abstract<\/jats:title>Purpose<\/jats:title>To investigate the impact of retinal blood vessels and retinal nerve fibre layer (RNFL<\/jats:styled-content>) segmentation errors on RNFL<\/jats:styled-content> measurement.<\/jats:p><\/jats:sec>Methods<\/jats:title>One eye of 180 subjects (60 normal, 66 mild\u2010to\u2010moderate and 54 advanced glaucoma subjects) was randomly selected for RNFL<\/jats:styled-content> imaging with a spectral\u2010domain OCT<\/jats:styled-content>. The boundaries of the RNFL<\/jats:styled-content> detected by the instrument software were checked, and the segmentation errors were corrected by a customized computer program. The differences in average and regional RNFL<\/jats:styled-content> thicknesses (RNFLT<\/jats:styled-content>) before and after the correction were analysed to determine the frequency of segmentation error (defined as an absolute difference in average RNFLT<\/jats:styled-content> >5.0\u00a0\u03bc<\/jats:italic>m). The ratio of retinal blood vessel cross\u2010sectional area to RNFL<\/jats:styled-content> cross\u2010sectional area was calculated.<\/jats:p><\/jats:sec>Results<\/jats:title>The difference in average RNFLT<\/jats:styled-content> (postsegmentation minus presegmentation refinement) ranged from \u22123.0 to 2.5\u00a0\u03bc<\/jats:italic>m (mean\u00a0\u00b1\u00a0standard deviation: 0.83\u00a0\u00b1\u00a00.86\u00a0\u03bc<\/jats:italic>m) in the normal, \u22122.5 to 5.0\u00a0\u03bc<\/jats:italic>m (0.56\u00a0\u00b1\u00a01.08\u00a0\u03bc<\/jats:italic>m) in the mild\u2010to\u2010moderate glaucoma and \u221211.0 to 9.5\u00a0\u03bc<\/jats:italic>m (0.05\u00a0\u00b1\u00a03.49\u00a0\u03bc<\/jats:italic>m) in the advanced glaucoma groups (p\u00a0=\u00a00.003). A total of 15% of eyes had average RNFLT<\/jats:styled-content> measurement error >5.0\u00a0\u03bc<\/jats:italic>m in the advanced glaucoma group. The proportion of retinal blood vessels in the RNFL<\/jats:styled-content> also increased with the severity of glaucoma (p\u00a0<\u00a00.001) with 4.2\u00a0\u00b1\u00a01.0% in the normal group, 4.9\u00a0\u00b1\u00a01.5% in the mild\u2010to\u2010moderate and 8.5\u00a0\u00b1\u00a03.5% in the advanced glaucoma groups.<\/jats:p><\/jats:sec>Conclusions<\/jats:title>Inclusion of retinal blood vessels and RNFL<\/jats:styled-content> segmentation error could adversely affect RNFL<\/jats:styled-content> measurement, particularly in advanced glaucoma when the RNFL<\/jats:styled-content> was thin.<\/jats:p><\/jats:sec>","DOI":"10.1111\/aos.12762","type":"journal-article","created":{"date-parts":[[2015,7,1]],"date-time":"2015-07-01T15:43:46Z","timestamp":1435765426000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":37,"title":["Impact of segmentation errors and retinal blood vessels on retinal nerve fibre layer measurements using spectral\u2010domain optical coherence tomography"],"prefix":"10.1111","volume":"94","author":[{"given":"Cong","family":"Ye","sequence":"first","affiliation":[{"name":"Department of Ophthalmology and Visual Sciences The Chinese University of Hong Kong Hong Kong China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marco","family":"Yu","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology and Visual Sciences The Chinese University of Hong Kong Hong Kong China"},{"name":"Department of Mathematics and Statistics Hang Seng Management College Hong Kong China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christopher Kai\u2010shun","family":"Leung","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology and Visual Sciences The Chinese University of Hong Kong Hong Kong China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2015,7]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.04-1174"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ophtha.2007.05.035"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1097\/WNO.0b013e31814b1ac4"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1097\/IAE.0b013e31829477f2"},{"key":"e_1_2_5_6_1","volume-title":"Clinical decisions in glaucoma","author":"Hodapp 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