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The levels of cytokines were assessed before and after heat\u2010induced inactivation. Individual serum preparations were tested for their anti\u2010inflammatory effect using an <jats:italic>in\u00a0vitro<\/jats:italic> test to differentiate effector T lymphocytes into anti\u2010inflammatory regulatory T cells.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The anti\u2010inflammatory cytokine <jats:styled-content style=\"fixed-case\">TGF<\/jats:styled-content>\u2010<jats:italic>\u03b2<\/jats:italic> was readily detected in the serum of all blood donors and was only modestly affected by heat\u2010induced inactivation. Serum containing high amounts of <jats:styled-content style=\"fixed-case\">TGF<\/jats:styled-content>\u2010<jats:italic>\u03b2<\/jats:italic> was more effective at inducing anti\u2010inflammatory regulatory T cells. The serum of one healthy blood donor displayed high levels of inflammatory cytokines.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>We propose that serum used as eye drops is screened for its cytokine content, making it possible to correlate the composition to the clinical outcome. Based on the findings in this study, tailored serum eye drops produced from allogeneic donors may provide increased anti\u2010inflammatory effects. This may be superior to autologous serum eye drops, which in many cases are retrieved from patients with inflammatory diseases.<\/jats:p><\/jats:sec>","DOI":"10.1111\/aos.12801","type":"journal-article","created":{"date-parts":[[2015,7,14]],"date-time":"2015-07-14T20:38:16Z","timestamp":1436906296000},"page":"654-657","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":38,"title":["Improving the anti\u2010inflammatory effect of serum eye drops using allogeneic serum permissive for regulatory T cell induction"],"prefix":"10.1111","volume":"93","author":[{"given":"Per\u2010Anton","family":"Stenwall","sequence":"first","affiliation":[{"name":"Department of Immunology, Genetics and Pathology Section\u00a0of Clinical Immunology Uppsala University Uppsala Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcus","family":"Bergstr\u00f6m","sequence":"additional","affiliation":[{"name":"Department of Immunology, 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