{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,31]],"date-time":"2025-10-31T07:38:21Z","timestamp":1761896301414},"reference-count":27,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2016,3,24]],"date-time":"2016-03-24T00:00:00Z","timestamp":1458777600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Acta Ophthalmologica"],"published-print":{"date-parts":[[2016,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Purpose<\/jats:title><jats:p>To evaluate serum cytokine profile from patients with active scleritis in a two\u2010centre prospective case\u2013control study.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>The serum of 20 active scleritis patients not treated with any local, periocular, or systemic immunomodulatory therapy (<jats:styled-content style=\"fixed-case\">IMT<\/jats:styled-content>) was analysed with multiplex assay to determine the levels of 11 cytokines interleukin (<jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>)\u20101<jats:italic>\u03b2<\/jats:italic>,<jats:styled-content style=\"fixed-case\"> IL<\/jats:styled-content>\u20106, <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u20102, <jats:styled-content style=\"fixed-case\">IFN<\/jats:styled-content>\u2010<jats:italic>\u03b3<\/jats:italic>,<jats:styled-content style=\"fixed-case\"> IL<\/jats:styled-content>\u201010, <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u201012p40, <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u201013, <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u201017A, <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u20105, <jats:styled-content style=\"fixed-case\">TNF<\/jats:styled-content>\u2010<jats:italic>\u03b1<\/jats:italic>, and <jats:styled-content style=\"fixed-case\">TNF<\/jats:styled-content>\u2010<jats:italic>\u03b2<\/jats:italic>, and with <jats:styled-content style=\"fixed-case\">ELISA<\/jats:styled-content> to determine the levels of <jats:styled-content style=\"fixed-case\">TGF<\/jats:styled-content>\u2010<jats:italic>\u03b2<\/jats:italic>1, <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u201022, and <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u201023. Twenty\u2010five age\u2010matched healthy volunteers were used as controls. In a subgroup of 13 patients with active disease, a second serum sample was obtained when the disease was inactive and levels of <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u201022 were determined. Serum <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u201022 levels from patients with active scleritis were correlated with type of scleritis (non\u2010necrotizing and necrotizing), degree of inflammation (0\u20134+\u00a0:\u22642+ and &gt;2+), and associated systemic disease.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Serum levels of <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u201022 were elevated in active scleritis patients compared to controls (6.41\u00a0\u00b1\u00a01.52\u00a0pg\/<jats:styled-content style=\"fixed-case\">ml<\/jats:styled-content> versus 1.93\u00a0\u00b1\u00a00.39\u00a0pg\/<jats:styled-content style=\"fixed-case\">ml<\/jats:styled-content>, p<jats:italic>\u00a0<\/jats:italic>=<jats:italic>\u00a0<\/jats:italic>0.012) and significantly decreased after scleritis remission with the use of <jats:styled-content style=\"fixed-case\">IMT<\/jats:styled-content> (p<jats:italic>\u00a0<\/jats:italic>=<jats:italic>\u00a0<\/jats:italic>0.005). There was no statistical association with scleritis type, degree of inflammation, or associated systemic disease. The serum levels of other cytokines were not significantly different from controls.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>In our study cohort, <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u201022 serum levels were significantly elevated in active scleritis patients compared to controls and decreased significantly after remission. Our results suggest that <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u201022, a T helper (Th) 17\u2010 and Th22\u2010 derived cytokine, may play a critical role in the physiopathology of scleritis.<\/jats:p><\/jats:sec>","DOI":"10.1111\/aos.13005","type":"journal-article","created":{"date-parts":[[2016,3,24]],"date-time":"2016-03-24T09:52:05Z","timestamp":1458813125000},"update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Interleukin\u201022 serum levels are elevated in active scleritis"],"prefix":"10.1111","volume":"94","author":[{"given":"Maite","family":"Sainz\u2010de\u2010la\u2010Maza","sequence":"first","affiliation":[{"name":"Institute Clinic of Ophthalmology Hospital Clinic of Barcelona University of Barcelona Barcelona Spain"},{"name":"The August Pi i Sunyer Biomedical Research Institute (IDIBAPS) Barcelona Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Blanca","family":"Molins","sequence":"additional","affiliation":[{"name":"The August Pi i Sunyer Biomedical Research Institute (IDIBAPS) Barcelona Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marina","family":"Mesquida","sequence":"additional","affiliation":[{"name":"Institute Clinic of Ophthalmology Hospital Clinic of Barcelona University of Barcelona Barcelona Spain"},{"name":"The August Pi i Sunyer Biomedical Research Institute (IDIBAPS) Barcelona Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Victor","family":"Lloren\u00e7","sequence":"additional","affiliation":[{"name":"Institute Clinic of Ophthalmology Hospital Clinic of Barcelona University of Barcelona Barcelona Spain"},{"name":"The August Pi i Sunyer Biomedical Research Institute (IDIBAPS) Barcelona Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Javier","family":"Zarranz\u2010Ventura","sequence":"additional","affiliation":[{"name":"Institute Clinic of Ophthalmology Hospital Clinic of Barcelona University of Barcelona Barcelona Spain"},{"name":"The August Pi i Sunyer Biomedical Research Institute (IDIBAPS) Barcelona Spain"},{"name":"Medical Retina and Uveitis Service Moorfields Eye Hospital London UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anna","family":"Sala\u2010Puigdollers","sequence":"additional","affiliation":[{"name":"Institute Clinic of Ophthalmology Hospital Clinic of Barcelona University of Barcelona Barcelona Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jessica","family":"Matas","sequence":"additional","affiliation":[{"name":"Institute Clinic of Ophthalmology Hospital Clinic of Barcelona University of Barcelona Barcelona Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alfredo","family":"Adan","sequence":"additional","affiliation":[{"name":"Institute Clinic of Ophthalmology Hospital Clinic of Barcelona University of Barcelona Barcelona Spain"},{"name":"The August Pi i Sunyer Biomedical Research Institute (IDIBAPS) Barcelona Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C. Stephen","family":"Foster","sequence":"additional","affiliation":[{"name":"Massachusetts Eye Research and Surgery Institution (MERSI) Waltham MA USA"},{"name":"Ocular Immunology and Uveitis Foundation (OIUF) Waltham MA USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2016,3,24]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1093\/rheumatology\/keq224"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm1585"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1136\/bjo.78.5.381"},{"key":"e_1_2_5_5_1","first-page":"653","article-title":"Increased expression of matrix metalloproteinases in\u00a0vivo in scleritis tissue and in\u00a0vitro in cultured human scleral fibroblasts","volume":"150","author":"Di Girolamo N","year":"1997","journal-title":"Am J Pathol"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/jlb.61.6.667"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1521-4141(199806)28:06<1773::AID-IMMU1773>3.0.CO;2-B"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0161-6420(91)32280-2"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10753-013-9715-y"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/art.33446"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1136\/ard.2011.152074"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.181.7.5147"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/art.34329"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molimm.2011.08.020"},{"key":"e_1_2_5_15_1","doi-asserted-by":"publisher","DOI":"10.1155\/2013\/968549"},{"key":"e_1_2_5_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cyto.2014.10.006"},{"key":"e_1_2_5_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0161-6420(94)31178-X"},{"key":"e_1_2_5_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ophtha.2011.07.013"},{"key":"e_1_2_5_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ophtha.2011.07.043"},{"key":"e_1_2_5_20_1","doi-asserted-by":"publisher","DOI":"10.1002\/art.34477"},{"key":"e_1_2_5_21_1","doi-asserted-by":"publisher","DOI":"10.1001\/archopht.116.11.1476"},{"key":"e_1_2_5_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ophtha.2010.08.027"},{"key":"e_1_2_5_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ophtha.2014.04.044"},{"key":"e_1_2_5_24_1","doi-asserted-by":"publisher","DOI":"10.1136\/bjo.2007.126425"},{"key":"e_1_2_5_25_1","doi-asserted-by":"publisher","DOI":"10.1002\/art.30314"},{"key":"e_1_2_5_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cytogfr.2010.08.002"},{"key":"e_1_2_5_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.autrev.2013.11.008"},{"key":"e_1_2_5_28_1","doi-asserted-by":"publisher","DOI":"10.1111\/1756-185X.12099"}],"container-title":["Acta Ophthalmologica"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Faos.13005","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/aos.13005","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,23]],"date-time":"2023-09-23T16:45:41Z","timestamp":1695487541000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/aos.13005"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,3,24]]},"references-count":27,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2016,9]]}},"alternative-id":["10.1111\/aos.13005"],"URL":"https:\/\/doi.org\/10.1111\/aos.13005","archive":["Portico"],"relation":{},"ISSN":["1755-375X","1755-3768"],"issn-type":[{"type":"print","value":"1755-375X"},{"type":"electronic","value":"1755-3768"}],"subject":[],"published":{"date-parts":[[2016,3,24]]},"assertion":[{"value":"2015-09-07","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2016-01-07","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2016-03-24","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}