{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,20]],"date-time":"2026-08-20T16:13:32Z","timestamp":1787242412663,"version":"build-2736575974"},"reference-count":55,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2017,2,15]],"date-time":"2017-02-15T00:00:00Z","timestamp":1487116800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Acta Ophthalmologica"],"published-print":{"date-parts":[[2017,11]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Purpose<\/jats:title>\n                    <jats:p>\n                      The aim of this study was to test the efficacy of Neurokinin\u20101 Receptor (\n                      <jats:styled-content style=\"fixed-case\">NK<\/jats:styled-content>\n                      \u20101R) antagonist \u2212Fosaprepitant\u2212 in inducing regression of established corneal neovascularization (\n                      <jats:styled-content style=\"fixed-case\">CNV<\/jats:styled-content>\n                      ).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Twenty C57\n                      <jats:styled-content style=\"fixed-case\">BL<\/jats:styled-content>\n                      \/6 mice underwent alkali burn. Seven days later, when corneal neovessels had developed, they received Fosaprepitant 10\u00a0mg\/ml, administered topically six times a day in the right eye for 10\u00a0days. In parallel, a group of 20 causticated mice was treated with normal saline, as control. A second independent experiment was also performed (\n                      <jats:italic>n<\/jats:italic>\n                      \u00a0=\u00a010\/group). Finally, ten healthy mice received the same topical treatment for 10\u00a0days to evaluate Fosaprepitant safety. Haemangiogenesis and lymphangiogenesis were measured by means of\n                      <jats:sc>vesselj<\/jats:sc>\n                      plugin (\n                      <jats:sc>imagej<\/jats:sc>\n                      ). Secondary endpoints, such as leucocyte infiltration, corneal opacity and corneal fluorescein staining were also evaluated. Inflammatory cell composition was assessed by flow cytometry.\n                    <\/jats:p>\n                    <jats:p>\n                      Differences between groups were assessed using unpaired\n                      <jats:italic>t<\/jats:italic>\n                      \u2010test, Mann\u2013Whitney\n                      <jats:italic>U<\/jats:italic>\n                      \u2010test or two\u2010way\n                      <jats:sc>anova<\/jats:sc>\n                      , as appropriate.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Topical Fosaprepitant administration induced a significant reduction of (i)\n                      <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>\n                      31\n                      <jats:sup>+<\/jats:sup>\n                      blood corneal neovessels (\u221227%, p\u00a0=\u00a00.0132), (ii)\n                      <jats:styled-content style=\"fixed-case\">LYVE<\/jats:styled-content>\n                      1\n                      <jats:sup>+<\/jats:sup>\n                      lymphatic corneal neovessels (\u221231%, p\u00a0=\u00a00.0118) and (iii)\n                      <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>\n                      45\n                      <jats:sup>+<\/jats:sup>\n                      leucocyte infiltration (\u221236%; p\u00a0=\u00a00.0237). The second independent experiment confirmed these data. Moreover, Fosaprepitant\u2010treated corneas showed a reduction in opacity, no impairment in corneal fluorescein staining and decreased infiltration of neutrophils (\u221272%, p\u00a0&lt;\u00a00.05) and macrophages (\u221275%, p\u00a0&lt;\u00a00.01). Finally, topical Fosaprepitant was not toxic to the ocular surface: no signs of conjunctivitis, opacity, perforations or corneal fluorescein staining were detected. Similarly, corneal\n                      <jats:styled-content style=\"fixed-case\">TUJ<\/jats:styled-content>\n                      1\n                      <jats:sup>+<\/jats:sup>\n                      nerve density was not affected.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>\n                      Our data suggest that\n                      <jats:styled-content style=\"fixed-case\">NK<\/jats:styled-content>\n                      \u20101R antagonists, such as Fosaprepitant, could be a new, promising therapeutic tool to inhibit\n                      <jats:styled-content style=\"fixed-case\">CNV<\/jats:styled-content>\n                      after this has been established.\n                    <\/jats:p>\n                  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